Comprehensive protocol for preparing diatom cell samples and associated bacterial consortia for scanning electron microscopy
Summary: Meticulous sample preparation and strict adherence to preservation procedures are essential for electron microscopy investigations, which enable accurate capture of organisms’ morphology, size, and potential interactions within the sample. Here, we present a protocol for preserving cells of...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-12-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166724005458 |
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| author | Margaux Maillard Nicolas Stephant Atsuko Tanaka Leïla Tirichine |
| author_facet | Margaux Maillard Nicolas Stephant Atsuko Tanaka Leïla Tirichine |
| author_sort | Margaux Maillard |
| collection | DOAJ |
| description | Summary: Meticulous sample preparation and strict adherence to preservation procedures are essential for electron microscopy investigations, which enable accurate capture of organisms’ morphology, size, and potential interactions within the sample. Here, we present a protocol for preserving cells of the model diatom Phaeodactylum tricornutum and its native bacterial community. We describe steps for diatom fixation and coverslip preparation and washing. We then detail procedures for dehydrating, drying, and metallizing samples followed by observation using scanning electron microscopy. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-076e5544ab8d48979ca6e21f671d4b60 |
| institution | Kabale University |
| issn | 2666-1667 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-076e5544ab8d48979ca6e21f671d4b602024-11-20T05:08:00ZengElsevierSTAR Protocols2666-16672024-12-0154103380Comprehensive protocol for preparing diatom cell samples and associated bacterial consortia for scanning electron microscopyMargaux Maillard0Nicolas Stephant1Atsuko Tanaka2Leïla Tirichine3Nantes Université, CNRS, US2B, UMR 6286, 44000 Nantes, FranceNantes Université, CNRS, Institut des Matériaux de Nantes Jean Rouxel, IMN, 44000 Nantes, FranceDepartment of Chemistry, Biology and Marine Science, Faculty of Science, University of the Ryukyus, Okinawa 903-0213, Japan; Corresponding authorNantes Université, CNRS, US2B, UMR 6286, 44000 Nantes, France; Institute for Marine and Antarctic Studies (IMAS), Ecology and Biodiversity Centre, University of Tasmania, Hobart, TAS 7004, Australia; Corresponding authorSummary: Meticulous sample preparation and strict adherence to preservation procedures are essential for electron microscopy investigations, which enable accurate capture of organisms’ morphology, size, and potential interactions within the sample. Here, we present a protocol for preserving cells of the model diatom Phaeodactylum tricornutum and its native bacterial community. We describe steps for diatom fixation and coverslip preparation and washing. We then detail procedures for dehydrating, drying, and metallizing samples followed by observation using scanning electron microscopy. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166724005458MicrobiologyMicroscopyModel Organisms |
| spellingShingle | Margaux Maillard Nicolas Stephant Atsuko Tanaka Leïla Tirichine Comprehensive protocol for preparing diatom cell samples and associated bacterial consortia for scanning electron microscopy STAR Protocols Microbiology Microscopy Model Organisms |
| title | Comprehensive protocol for preparing diatom cell samples and associated bacterial consortia for scanning electron microscopy |
| title_full | Comprehensive protocol for preparing diatom cell samples and associated bacterial consortia for scanning electron microscopy |
| title_fullStr | Comprehensive protocol for preparing diatom cell samples and associated bacterial consortia for scanning electron microscopy |
| title_full_unstemmed | Comprehensive protocol for preparing diatom cell samples and associated bacterial consortia for scanning electron microscopy |
| title_short | Comprehensive protocol for preparing diatom cell samples and associated bacterial consortia for scanning electron microscopy |
| title_sort | comprehensive protocol for preparing diatom cell samples and associated bacterial consortia for scanning electron microscopy |
| topic | Microbiology Microscopy Model Organisms |
| url | http://www.sciencedirect.com/science/article/pii/S2666166724005458 |
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