Synthesis and characterization of innovative GA@Ag-CuO nanocomposite with potent antimicrobial and anticancer properties
Abstract Cancer and microbial infections place a significant burden on the world’s health systems and can increase the rate of disease and mortality. In the current study, a novel nanocomposite based on Gum Arabic, silver and copper oxide nanoparticles (GA@Ag-CuO nanocomposite) was synthesized to ov...
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| Format: | Article |
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Nature Portfolio
2025-01-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-76446-2 |
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| author | Amr H. Hashem Mostafa A. Abdel-Maksoud Sabiha Fatima Saeedah M. Almutairi Mohamed A. Ghorab Ahmed I. El-Batal Gharieb S. El-Sayyad |
| author_facet | Amr H. Hashem Mostafa A. Abdel-Maksoud Sabiha Fatima Saeedah M. Almutairi Mohamed A. Ghorab Ahmed I. El-Batal Gharieb S. El-Sayyad |
| author_sort | Amr H. Hashem |
| collection | DOAJ |
| description | Abstract Cancer and microbial infections place a significant burden on the world’s health systems and can increase the rate of disease and mortality. In the current study, a novel nanocomposite based on Gum Arabic, silver and copper oxide nanoparticles (GA@Ag-CuO nanocomposite) was synthesized to overcome the problem of microbial infection and in cancer treatment. Characterization using UV-Vis. spectrophotometer reveals that, the observed peak in the spectrum was formed by the observed O.D. at 0.755, and confirmed that the produced GA@Ag-CuO nanocomposite was small and discernible at 360 nm. The particles’ diameters varied from 9.5 nm to 49.5 nm, with a mean diameter of 25.53 ± 1.4 nm. The created Gum Arabic filtrate was rich in active functional groups, and the provided polydisperse NPs were intended to reduce, stabilize, and the produced filtrate act as capping agents. Based on the XRD data, the synthesized GA@Ag-CuO nanocomposite was crystallized and had a face-centered (fcc) crystal structure. Biosafety of GA@Ag-CuO nanocomposite was assessed toward Wi 38 normal cell line, where it showed safety toward the tested cell line where IC50 was 154.2 µg/mL. Antimicrobial results confirmed that, GA@Ag-CuO nanocomposite has antibacterial activity with MICs 15.6, 125, 31.25 and 125 µg/mL against S. epidermis, S. aureus, L. plantrum, and S. typhimurium, respectively. Likewise, it showed antifungal activity toward C. albicans and C. neoformans with MICs 62.5 and 15.62 µg/ml, respectively. Moreover, GA@Ag-CuO nanocomposite displayed promising anticancer activity with IC50 26.11 and 59.5 µg/ml toward MCF-7 and Hep-G2, respectively. In conclusion, the novel GA@Ag-CuO nanocomposite demonstrated promising antibacterial, antifungal, and anticancer activities. |
| format | Article |
| id | doaj-art-0722f0e7dd0d4712a40d26ceefde60d0 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-0722f0e7dd0d4712a40d26ceefde60d02025-01-05T12:18:14ZengNature PortfolioScientific Reports2045-23222025-01-0115111710.1038/s41598-024-76446-2Synthesis and characterization of innovative GA@Ag-CuO nanocomposite with potent antimicrobial and anticancer propertiesAmr H. Hashem0Mostafa A. Abdel-Maksoud1Sabiha Fatima2Saeedah M. Almutairi3Mohamed A. Ghorab4Ahmed I. El-Batal5Gharieb S. El-Sayyad6Botany and Microbiology Department, Faculty of Science, Al-Azhar UniversityDepartment of Botany and Microbiology, College of Science, King Saud UniversityDepartment of Clinical Laboratory Science, College of Applied Medical Sciences, King Saud UniversityDepartment of Botany and Microbiology, College of Science, King Saud UniversityWildlife Toxicology Lab, Dept. of Animal Science, Institute for Integrative Toxicology (IIT), Michigan State UniversityDrug Microbiology Lab, Drug Radiation Research Department, National Center for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority (EAEA)Department of Medical Laboratory Technology, Faculty of Applied Health Sciences Technology, Badr University in Cairo (BUC)Abstract Cancer and microbial infections place a significant burden on the world’s health systems and can increase the rate of disease and mortality. In the current study, a novel nanocomposite based on Gum Arabic, silver and copper oxide nanoparticles (GA@Ag-CuO nanocomposite) was synthesized to overcome the problem of microbial infection and in cancer treatment. Characterization using UV-Vis. spectrophotometer reveals that, the observed peak in the spectrum was formed by the observed O.D. at 0.755, and confirmed that the produced GA@Ag-CuO nanocomposite was small and discernible at 360 nm. The particles’ diameters varied from 9.5 nm to 49.5 nm, with a mean diameter of 25.53 ± 1.4 nm. The created Gum Arabic filtrate was rich in active functional groups, and the provided polydisperse NPs were intended to reduce, stabilize, and the produced filtrate act as capping agents. Based on the XRD data, the synthesized GA@Ag-CuO nanocomposite was crystallized and had a face-centered (fcc) crystal structure. Biosafety of GA@Ag-CuO nanocomposite was assessed toward Wi 38 normal cell line, where it showed safety toward the tested cell line where IC50 was 154.2 µg/mL. Antimicrobial results confirmed that, GA@Ag-CuO nanocomposite has antibacterial activity with MICs 15.6, 125, 31.25 and 125 µg/mL against S. epidermis, S. aureus, L. plantrum, and S. typhimurium, respectively. Likewise, it showed antifungal activity toward C. albicans and C. neoformans with MICs 62.5 and 15.62 µg/ml, respectively. Moreover, GA@Ag-CuO nanocomposite displayed promising anticancer activity with IC50 26.11 and 59.5 µg/ml toward MCF-7 and Hep-G2, respectively. In conclusion, the novel GA@Ag-CuO nanocomposite demonstrated promising antibacterial, antifungal, and anticancer activities.https://doi.org/10.1038/s41598-024-76446-2Gum arabicBimetallic Ag-CuO NPsAntimicrobial potentialAnticancer activity |
| spellingShingle | Amr H. Hashem Mostafa A. Abdel-Maksoud Sabiha Fatima Saeedah M. Almutairi Mohamed A. Ghorab Ahmed I. El-Batal Gharieb S. El-Sayyad Synthesis and characterization of innovative GA@Ag-CuO nanocomposite with potent antimicrobial and anticancer properties Scientific Reports Gum arabic Bimetallic Ag-CuO NPs Antimicrobial potential Anticancer activity |
| title | Synthesis and characterization of innovative GA@Ag-CuO nanocomposite with potent antimicrobial and anticancer properties |
| title_full | Synthesis and characterization of innovative GA@Ag-CuO nanocomposite with potent antimicrobial and anticancer properties |
| title_fullStr | Synthesis and characterization of innovative GA@Ag-CuO nanocomposite with potent antimicrobial and anticancer properties |
| title_full_unstemmed | Synthesis and characterization of innovative GA@Ag-CuO nanocomposite with potent antimicrobial and anticancer properties |
| title_short | Synthesis and characterization of innovative GA@Ag-CuO nanocomposite with potent antimicrobial and anticancer properties |
| title_sort | synthesis and characterization of innovative ga ag cuo nanocomposite with potent antimicrobial and anticancer properties |
| topic | Gum arabic Bimetallic Ag-CuO NPs Antimicrobial potential Anticancer activity |
| url | https://doi.org/10.1038/s41598-024-76446-2 |
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