Enhancement of antitumor effects of berberine chloride with a copper(II) complex against human triple negative breast cancer: In vitro studies
In this study, the copper(II) complex [Cu(chromoneTSC)Cl2]•0.5H2O•0.0625C2H5OH (where chromoneTSC = (E)-N-Ethyl-2-((4-oxo-4H-chromen-3-yl)methylene)-hydrazinecarbothioamide) was synthesized and characterized; then used to carry out in vitro studies in combination with berberine chloride (BBC). The l...
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Elsevier
2024-12-01
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| Series: | Results in Chemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211715624005782 |
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| author | Duaa R. Alajroush Brittney F. Anderson Janae A. Bruce Christian I. Lartey Dazonte A. Mathurin Sean T. Washington Tanaya S. Washington Sidy Diawara Sakariyau A. Waheed Kaylin L. Thomas Stephen J. Beebe Alvin A. Holder |
| author_facet | Duaa R. Alajroush Brittney F. Anderson Janae A. Bruce Christian I. Lartey Dazonte A. Mathurin Sean T. Washington Tanaya S. Washington Sidy Diawara Sakariyau A. Waheed Kaylin L. Thomas Stephen J. Beebe Alvin A. Holder |
| author_sort | Duaa R. Alajroush |
| collection | DOAJ |
| description | In this study, the copper(II) complex [Cu(chromoneTSC)Cl2]•0.5H2O•0.0625C2H5OH (where chromoneTSC = (E)-N-Ethyl-2-((4-oxo-4H-chromen-3-yl)methylene)-hydrazinecarbothioamide) was synthesized and characterized; then used to carry out in vitro studies in combination with berberine chloride (BBC). The ligand and complex were characterized by elemental analysis, FTIR and NMR (1H and 13C) spectroscopy, and conductivity measurements. The cytotoxic effect was analyzed by using the CCK-8 viability assay in cancer MDA-MB-231 VIM RFP and non-cancer MCF-10A cell lines. The IC50 values for the complex and BBC were 21.2 ± 1.6 and 48.3 ± 2.4 μM, respectively at 24 h incubation, while the IC50 value of the combination treatment was 9.3 ± 1.5 in cancer cells. The co-treatment group significantly increased the number of cells in G2 phase, indicating the growth arrest of cancer cells. Moreover, the combination group showed induction of both intrinsic and extrinsic apoptotic pathways. There was also a study on the effect of the combination treatment on receptor-interacting serine/threonine-protein kinase 3 (RIPK3) and mixed lineage kinase domain-like pseudokinase (MLKL) as biomarkers of necroptosis. The results showed activation of necroptosis after treatment with the combination of the copper complex and BBC via the activation of RIPK3–MLKL pathway. |
| format | Article |
| id | doaj-art-070027bb9ed248a38b1ca13fd1023e0f |
| institution | Kabale University |
| issn | 2211-7156 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Results in Chemistry |
| spelling | doaj-art-070027bb9ed248a38b1ca13fd1023e0f2024-12-11T05:56:19ZengElsevierResults in Chemistry2211-71562024-12-0112101882Enhancement of antitumor effects of berberine chloride with a copper(II) complex against human triple negative breast cancer: In vitro studiesDuaa R. Alajroush0Brittney F. Anderson1Janae A. Bruce2Christian I. Lartey3Dazonte A. Mathurin4Sean T. Washington5Tanaya S. Washington6Sidy Diawara7Sakariyau A. Waheed8Kaylin L. Thomas9Stephen J. Beebe10Alvin A. Holder11Department of Chemistry and Biochemistry, Old Dominion University 4501 Elkhorn Avenue, Norfolk, VA 23529, USADepartment of Biological Sciences, University of the Virgin Islands, 2 John Brewers Bay, St. Thomas, VI 00802, USADepartment of Biological Sciences, University of the Virgin Islands, 2 John Brewers Bay, St. Thomas, VI 00802, USADepartment of Chemistry and Biochemistry, Old Dominion University 4501 Elkhorn Avenue, Norfolk, VA 23529, USADepartment of Biological Sciences, University of the Virgin Islands, 2 John Brewers Bay, St. Thomas, VI 00802, USADepartment of Chemistry and Biochemistry, Old Dominion University 4501 Elkhorn Avenue, Norfolk, VA 23529, USADepartment of Chemistry and Biochemistry, Old Dominion University 4501 Elkhorn Avenue, Norfolk, VA 23529, USADepartment of Chemistry and Biochemistry, Old Dominion University 4501 Elkhorn Avenue, Norfolk, VA 23529, USADepartment of Chemistry and Biochemistry, Old Dominion University 4501 Elkhorn Avenue, Norfolk, VA 23529, USADepartment of Chemistry and Biochemistry, Old Dominion University 4501 Elkhorn Avenue, Norfolk, VA 23529, USAFrank Reidy Research center for Bioelectrics, Old Dominion University, 4211 Monarch Way, Suite 300, Norfolk, VA, 23508, USADepartment of Chemistry and Biochemistry, Old Dominion University 4501 Elkhorn Avenue, Norfolk, VA 23529, USA; Corresponding author at: Department of Chemistry and Biochemistry, Old Dominion University 4501 Elkhorn Avenue, Norfolk, VA 23529, USA.In this study, the copper(II) complex [Cu(chromoneTSC)Cl2]•0.5H2O•0.0625C2H5OH (where chromoneTSC = (E)-N-Ethyl-2-((4-oxo-4H-chromen-3-yl)methylene)-hydrazinecarbothioamide) was synthesized and characterized; then used to carry out in vitro studies in combination with berberine chloride (BBC). The ligand and complex were characterized by elemental analysis, FTIR and NMR (1H and 13C) spectroscopy, and conductivity measurements. The cytotoxic effect was analyzed by using the CCK-8 viability assay in cancer MDA-MB-231 VIM RFP and non-cancer MCF-10A cell lines. The IC50 values for the complex and BBC were 21.2 ± 1.6 and 48.3 ± 2.4 μM, respectively at 24 h incubation, while the IC50 value of the combination treatment was 9.3 ± 1.5 in cancer cells. The co-treatment group significantly increased the number of cells in G2 phase, indicating the growth arrest of cancer cells. Moreover, the combination group showed induction of both intrinsic and extrinsic apoptotic pathways. There was also a study on the effect of the combination treatment on receptor-interacting serine/threonine-protein kinase 3 (RIPK3) and mixed lineage kinase domain-like pseudokinase (MLKL) as biomarkers of necroptosis. The results showed activation of necroptosis after treatment with the combination of the copper complex and BBC via the activation of RIPK3–MLKL pathway.http://www.sciencedirect.com/science/article/pii/S2211715624005782Triple negative breast cancerBerberine chloridecopper(II) complexThiosemicarbazonesCell death mechanisms |
| spellingShingle | Duaa R. Alajroush Brittney F. Anderson Janae A. Bruce Christian I. Lartey Dazonte A. Mathurin Sean T. Washington Tanaya S. Washington Sidy Diawara Sakariyau A. Waheed Kaylin L. Thomas Stephen J. Beebe Alvin A. Holder Enhancement of antitumor effects of berberine chloride with a copper(II) complex against human triple negative breast cancer: In vitro studies Results in Chemistry Triple negative breast cancer Berberine chloride copper(II) complex Thiosemicarbazones Cell death mechanisms |
| title | Enhancement of antitumor effects of berberine chloride with a copper(II) complex against human triple negative breast cancer: In vitro studies |
| title_full | Enhancement of antitumor effects of berberine chloride with a copper(II) complex against human triple negative breast cancer: In vitro studies |
| title_fullStr | Enhancement of antitumor effects of berberine chloride with a copper(II) complex against human triple negative breast cancer: In vitro studies |
| title_full_unstemmed | Enhancement of antitumor effects of berberine chloride with a copper(II) complex against human triple negative breast cancer: In vitro studies |
| title_short | Enhancement of antitumor effects of berberine chloride with a copper(II) complex against human triple negative breast cancer: In vitro studies |
| title_sort | enhancement of antitumor effects of berberine chloride with a copper ii complex against human triple negative breast cancer in vitro studies |
| topic | Triple negative breast cancer Berberine chloride copper(II) complex Thiosemicarbazones Cell death mechanisms |
| url | http://www.sciencedirect.com/science/article/pii/S2211715624005782 |
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