Sustainable fungi-based protein extraction from agro-waste mushroom stem using deep eutectic solvents
This study evaluated the nutritional and functional qualities of the mushroom stem (MS) powder and its protein concentrates, which were extracted using alkaline and deep eutectic solvent (DES)-based methods. MS powder is rich in protein (23.02 %) and carbohydrates (63.01 %). Alkaline extraction yiel...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-12-01
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| Series: | Food Chemistry: X |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590157524008198 |
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| author | Gulsah Karabulut Deniz Günal Köroğlu Hao Feng Zekeriya Karabulut |
| author_facet | Gulsah Karabulut Deniz Günal Köroğlu Hao Feng Zekeriya Karabulut |
| author_sort | Gulsah Karabulut |
| collection | DOAJ |
| description | This study evaluated the nutritional and functional qualities of the mushroom stem (MS) powder and its protein concentrates, which were extracted using alkaline and deep eutectic solvent (DES)-based methods. MS powder is rich in protein (23.02 %) and carbohydrates (63.01 %). Alkaline extraction yielded the highest protein content (45.90 %) but lower recovery rates (15.39 %) compared to DES extractions using a 1:2 molar ratio of choline chloride: glycerol (DES-CG) and choline chloride: lactic acid (DES-CL), which had recovery rates of 22.04 % and 21.47 %, respectively. DES solvents provided strong hydrogen bonding, as indicated by FTIR analysis, and produced uniform, smooth particles according to SEM imaging. Both DES and alkaline methods achieved similar protein solubility values (72–75 %). However, DES methods better preserved emulsion capabilities and balanced foaming properties, enhancing foam capacity and maintaining stability. The findings suggest that DES methods are more sustainable and efficient for extracting proteins from MS powder, optimizing both functional properties and environmental sustainability in food applications. |
| format | Article |
| id | doaj-art-06f6b4483d81471bba9909831591d2df |
| institution | Kabale University |
| issn | 2590-1575 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj-art-06f6b4483d81471bba9909831591d2df2024-12-13T11:01:51ZengElsevierFood Chemistry: X2590-15752024-12-0124101931Sustainable fungi-based protein extraction from agro-waste mushroom stem using deep eutectic solventsGulsah Karabulut0Deniz Günal Köroğlu1Hao Feng2Zekeriya Karabulut3Department of Food Engineering, Faculty of Engineering, Sakarya University, 54050 Sakarya, Türkiye; Corresponding author.Department of Food Engineering, Faculty of Chemical and Metallurgical Engineering, Istanbul Technical University, 34469 Istanbul, TürkiyeDepartment of Family and Consumer Sciences, North Carolina A&T State University, Greensboro, North Caroline 27411, USADepartment of Chemistry, Faculty of Arts and Sciences, Sakarya University, 54050 Sakarya, TürkiyeThis study evaluated the nutritional and functional qualities of the mushroom stem (MS) powder and its protein concentrates, which were extracted using alkaline and deep eutectic solvent (DES)-based methods. MS powder is rich in protein (23.02 %) and carbohydrates (63.01 %). Alkaline extraction yielded the highest protein content (45.90 %) but lower recovery rates (15.39 %) compared to DES extractions using a 1:2 molar ratio of choline chloride: glycerol (DES-CG) and choline chloride: lactic acid (DES-CL), which had recovery rates of 22.04 % and 21.47 %, respectively. DES solvents provided strong hydrogen bonding, as indicated by FTIR analysis, and produced uniform, smooth particles according to SEM imaging. Both DES and alkaline methods achieved similar protein solubility values (72–75 %). However, DES methods better preserved emulsion capabilities and balanced foaming properties, enhancing foam capacity and maintaining stability. The findings suggest that DES methods are more sustainable and efficient for extracting proteins from MS powder, optimizing both functional properties and environmental sustainability in food applications.http://www.sciencedirect.com/science/article/pii/S2590157524008198Mushroom proteinDeep eutectic solventsAlternative proteinFunctional propertiesGreen extraction |
| spellingShingle | Gulsah Karabulut Deniz Günal Köroğlu Hao Feng Zekeriya Karabulut Sustainable fungi-based protein extraction from agro-waste mushroom stem using deep eutectic solvents Food Chemistry: X Mushroom protein Deep eutectic solvents Alternative protein Functional properties Green extraction |
| title | Sustainable fungi-based protein extraction from agro-waste mushroom stem using deep eutectic solvents |
| title_full | Sustainable fungi-based protein extraction from agro-waste mushroom stem using deep eutectic solvents |
| title_fullStr | Sustainable fungi-based protein extraction from agro-waste mushroom stem using deep eutectic solvents |
| title_full_unstemmed | Sustainable fungi-based protein extraction from agro-waste mushroom stem using deep eutectic solvents |
| title_short | Sustainable fungi-based protein extraction from agro-waste mushroom stem using deep eutectic solvents |
| title_sort | sustainable fungi based protein extraction from agro waste mushroom stem using deep eutectic solvents |
| topic | Mushroom protein Deep eutectic solvents Alternative protein Functional properties Green extraction |
| url | http://www.sciencedirect.com/science/article/pii/S2590157524008198 |
| work_keys_str_mv | AT gulsahkarabulut sustainablefungibasedproteinextractionfromagrowastemushroomstemusingdeepeutecticsolvents AT denizgunalkoroglu sustainablefungibasedproteinextractionfromagrowastemushroomstemusingdeepeutecticsolvents AT haofeng sustainablefungibasedproteinextractionfromagrowastemushroomstemusingdeepeutecticsolvents AT zekeriyakarabulut sustainablefungibasedproteinextractionfromagrowastemushroomstemusingdeepeutecticsolvents |