Novel Insight into the Composition Differences Between Buffalo and Holstein Milk and Potential Anti-Inflammation and Antioxidant Effect on Caco-2 Cells
Milk is one of the most common sources of nutrients in humans, however, the composition and healthy value of the milk derived from different animals are very different. Here, we systemically compared the protein and lipid profiles and evaluated the anti-inflammation and antioxidant effect of buffalo...
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2024-12-01
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| Series: | Foods |
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| author | Luyao Wang Xinyue Hu Jiaqi Jiang Dong Wang Chaobin Qin Ling Li Deshun Shi Qingyou Liu Jian Wang Hui Li Jieping Huang Zhipeng Li |
| author_facet | Luyao Wang Xinyue Hu Jiaqi Jiang Dong Wang Chaobin Qin Ling Li Deshun Shi Qingyou Liu Jian Wang Hui Li Jieping Huang Zhipeng Li |
| author_sort | Luyao Wang |
| collection | DOAJ |
| description | Milk is one of the most common sources of nutrients in humans, however, the composition and healthy value of the milk derived from different animals are very different. Here, we systemically compared the protein and lipid profiles and evaluated the anti-inflammation and antioxidant effect of buffalo and Holstein-derived milk on Caco-2 cells. Results showed that 906 proteins and 1899 lipids were identified in the buffalo milk and Holstein milk samples including 161 significantly different proteins (DEPs) and 49 significantly different lipids. The DEPs were mainly enriched in defense response-related terms, while the differential lipids were mainly included in fat digestion and absorption and cholesterol metabolism pathways. In addition, the Caco-2 cells co-cultured with buffalo and Holstein milk components showed significant benefits in being resistant to LPS-induced inflammation stress and H<sub>2</sub>O<sub>2</sub>-induced ROS stress. The qRT-PCR and ELISA results showed that the expression of TNF-α, IL-1β, and IL-6 was significantly lower (<i>p</i> < 0.05) in the cells co-cultured with milk components. Further analysis showed that, after H<sub>2</sub>O<sub>2</sub> treatment, the expression of keap1 and Nrf-2 in the Caco-2 cells co-cultured with milk components was significantly lower (<i>p</i> < 0.05). In addition, being co-cultured with milk components significantly decreased the SOD, MDA, CAT, and GSH-Px content (<i>p</i> < 0.05) in the Caco-2 cells induced by H<sub>2</sub>O<sub>2</sub>. This study provides a novel insight into the differences in proteins and lipids between buffalo milk and Holstein milk, and a reference understanding of the anti-inflammation and antioxidant effect of the consumption of milk on the intestines. |
| format | Article |
| id | doaj-art-06d50394059742a782647d6b0f2be2ed |
| institution | Kabale University |
| issn | 2304-8158 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj-art-06d50394059742a782647d6b0f2be2ed2024-12-13T16:27:01ZengMDPI AGFoods2304-81582024-12-011323391510.3390/foods13233915Novel Insight into the Composition Differences Between Buffalo and Holstein Milk and Potential Anti-Inflammation and Antioxidant Effect on Caco-2 CellsLuyao Wang0Xinyue Hu1Jiaqi Jiang2Dong Wang3Chaobin Qin4Ling Li5Deshun Shi6Qingyou Liu7Jian Wang8Hui Li9Jieping Huang10Zhipeng Li11Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, College of Animal Science and Technology, Guangxi University, Nanning 530004, ChinaGuangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, College of Animal Science and Technology, Guangxi University, Nanning 530004, ChinaGuangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, College of Animal Science and Technology, Guangxi University, Nanning 530004, ChinaGuangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, College of Animal Science and Technology, Guangxi University, Nanning 530004, ChinaGuangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, College of Animal Science and Technology, Guangxi University, Nanning 530004, ChinaGuangxi Key Laboratory of Buffalo Genetics, Reproduction and Breeding, Guangxi Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning 530001, ChinaGuangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, College of Animal Science and Technology, Guangxi University, Nanning 530004, ChinaGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Life Science and Engineering, Foshan University, Foshan 528225, ChinaGuangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, College of Animal Science and Technology, Guangxi University, Nanning 530004, ChinaGuangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, College of Animal Science and Technology, Guangxi University, Nanning 530004, ChinaGuangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, College of Animal Science and Technology, Guangxi University, Nanning 530004, ChinaGuangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, College of Animal Science and Technology, Guangxi University, Nanning 530004, ChinaMilk is one of the most common sources of nutrients in humans, however, the composition and healthy value of the milk derived from different animals are very different. Here, we systemically compared the protein and lipid profiles and evaluated the anti-inflammation and antioxidant effect of buffalo and Holstein-derived milk on Caco-2 cells. Results showed that 906 proteins and 1899 lipids were identified in the buffalo milk and Holstein milk samples including 161 significantly different proteins (DEPs) and 49 significantly different lipids. The DEPs were mainly enriched in defense response-related terms, while the differential lipids were mainly included in fat digestion and absorption and cholesterol metabolism pathways. In addition, the Caco-2 cells co-cultured with buffalo and Holstein milk components showed significant benefits in being resistant to LPS-induced inflammation stress and H<sub>2</sub>O<sub>2</sub>-induced ROS stress. The qRT-PCR and ELISA results showed that the expression of TNF-α, IL-1β, and IL-6 was significantly lower (<i>p</i> < 0.05) in the cells co-cultured with milk components. Further analysis showed that, after H<sub>2</sub>O<sub>2</sub> treatment, the expression of keap1 and Nrf-2 in the Caco-2 cells co-cultured with milk components was significantly lower (<i>p</i> < 0.05). In addition, being co-cultured with milk components significantly decreased the SOD, MDA, CAT, and GSH-Px content (<i>p</i> < 0.05) in the Caco-2 cells induced by H<sub>2</sub>O<sub>2</sub>. This study provides a novel insight into the differences in proteins and lipids between buffalo milk and Holstein milk, and a reference understanding of the anti-inflammation and antioxidant effect of the consumption of milk on the intestines.https://www.mdpi.com/2304-8158/13/23/3915milkproteomelipidomeanti-inflammationantioxidantCaco-2 cell |
| spellingShingle | Luyao Wang Xinyue Hu Jiaqi Jiang Dong Wang Chaobin Qin Ling Li Deshun Shi Qingyou Liu Jian Wang Hui Li Jieping Huang Zhipeng Li Novel Insight into the Composition Differences Between Buffalo and Holstein Milk and Potential Anti-Inflammation and Antioxidant Effect on Caco-2 Cells Foods milk proteome lipidome anti-inflammation antioxidant Caco-2 cell |
| title | Novel Insight into the Composition Differences Between Buffalo and Holstein Milk and Potential Anti-Inflammation and Antioxidant Effect on Caco-2 Cells |
| title_full | Novel Insight into the Composition Differences Between Buffalo and Holstein Milk and Potential Anti-Inflammation and Antioxidant Effect on Caco-2 Cells |
| title_fullStr | Novel Insight into the Composition Differences Between Buffalo and Holstein Milk and Potential Anti-Inflammation and Antioxidant Effect on Caco-2 Cells |
| title_full_unstemmed | Novel Insight into the Composition Differences Between Buffalo and Holstein Milk and Potential Anti-Inflammation and Antioxidant Effect on Caco-2 Cells |
| title_short | Novel Insight into the Composition Differences Between Buffalo and Holstein Milk and Potential Anti-Inflammation and Antioxidant Effect on Caco-2 Cells |
| title_sort | novel insight into the composition differences between buffalo and holstein milk and potential anti inflammation and antioxidant effect on caco 2 cells |
| topic | milk proteome lipidome anti-inflammation antioxidant Caco-2 cell |
| url | https://www.mdpi.com/2304-8158/13/23/3915 |
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