Hybrid hydrogels containing gradients in gold nanoparticles for localized delivery of mesenchymal stem cells and enhanced nerve tissues remodeling in vivo
Currently, most peripheral nerve injuries are incurable mainly due to excessive reactive oxygen species (ROS) generation in inflammatory tissues, which can further exacerbate localized tissue injury and cause chronic diseases. Although promising for promoting nerve regeneration, stem cell therapy st...
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Elsevier
2025-02-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006424004721 |
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| author | Jie Gao Yiduo Zhou Gang Xu Zhongqing Wei Liucheng Ding Wei Zhang Yi Huang |
| author_facet | Jie Gao Yiduo Zhou Gang Xu Zhongqing Wei Liucheng Ding Wei Zhang Yi Huang |
| author_sort | Jie Gao |
| collection | DOAJ |
| description | Currently, most peripheral nerve injuries are incurable mainly due to excessive reactive oxygen species (ROS) generation in inflammatory tissues, which can further exacerbate localized tissue injury and cause chronic diseases. Although promising for promoting nerve regeneration, stem cell therapy still suffers from abundant intrinsic limitations, mainly including excessive ROS in lesions and inefficient production of growth factors (GFs). Biomaterials that scavenge endogenous ROS and promote GFs secretion might overcome such limitations and thus are being increasingly investigated. Herein, firstly reported as specific ROS scavenging agents and paracrine stimulators, gold nanoparticles (GNPs) were incorporated in the chitosan/polyvinyl alcohol hydrogel networks. The GNPs/hydrogel composite can support the survival of mesenchymal stem cells (MSCs) with excellent expansion efficiency and protect MSCs in a simulated ROS microenvironment, decreasing the intracellular ROS levels and simultaneously enhancing cell viability. Moreover, biodegradable scaffolds, along with MSCs, were implanted into sciatic nerve defects in a rat model to show good application value in vivo. Our work demonstrated that the GNPs/hydrogel shows great promise in MSCs therapy for peripheral nerve injury with convincing biological evidence. |
| format | Article |
| id | doaj-art-06c44bc9899441048d437d92db9f078b |
| institution | Kabale University |
| issn | 2590-0064 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj-art-06c44bc9899441048d437d92db9f078b2025-01-17T04:52:08ZengElsevierMaterials Today Bio2590-00642025-02-0130101411Hybrid hydrogels containing gradients in gold nanoparticles for localized delivery of mesenchymal stem cells and enhanced nerve tissues remodeling in vivoJie Gao0Yiduo Zhou1Gang Xu2Zhongqing Wei3Liucheng Ding4Wei Zhang5Yi Huang6Department of Urology, Jiangnan University Affiliated Hospital, Medical College of Jiangnan University, Wuxi 214125, ChinaDepartment of Urology, Nanjing Medical University Second Affiliated Hospital, Nanjing Medical University, Nanjing 210003, China; Institute of Chemistry and Biochemistry, Free University of Berlin, Berlin 14195, GermanyJiangsu Key Laboratory of Advanced Metallic Materials, School of Materials Science and Engineering, Southeast University, Nanjing 211189, ChinaDepartment of Urology, Nanjing Medical University Second Affiliated Hospital, Nanjing Medical University, Nanjing 210003, ChinaDepartment of Urology, Nanjing Medical University Second Affiliated Hospital, Nanjing Medical University, Nanjing 210003, China; Corresponding author.Jiangsu Key Laboratory of Advanced Metallic Materials, School of Materials Science and Engineering, Southeast University, Nanjing 211189, China; Corresponding author.w69zhang@seu.edu.cnDepartment of Urology, Jiangnan University Affiliated Hospital, Medical College of Jiangnan University, Wuxi 214125, China; Corresponding author.Currently, most peripheral nerve injuries are incurable mainly due to excessive reactive oxygen species (ROS) generation in inflammatory tissues, which can further exacerbate localized tissue injury and cause chronic diseases. Although promising for promoting nerve regeneration, stem cell therapy still suffers from abundant intrinsic limitations, mainly including excessive ROS in lesions and inefficient production of growth factors (GFs). Biomaterials that scavenge endogenous ROS and promote GFs secretion might overcome such limitations and thus are being increasingly investigated. Herein, firstly reported as specific ROS scavenging agents and paracrine stimulators, gold nanoparticles (GNPs) were incorporated in the chitosan/polyvinyl alcohol hydrogel networks. The GNPs/hydrogel composite can support the survival of mesenchymal stem cells (MSCs) with excellent expansion efficiency and protect MSCs in a simulated ROS microenvironment, decreasing the intracellular ROS levels and simultaneously enhancing cell viability. Moreover, biodegradable scaffolds, along with MSCs, were implanted into sciatic nerve defects in a rat model to show good application value in vivo. Our work demonstrated that the GNPs/hydrogel shows great promise in MSCs therapy for peripheral nerve injury with convincing biological evidence.http://www.sciencedirect.com/science/article/pii/S2590006424004721HydrogelGold nanoparticlesMesenchymal stem cellsPeripheral nerve injuryNeural regeneration |
| spellingShingle | Jie Gao Yiduo Zhou Gang Xu Zhongqing Wei Liucheng Ding Wei Zhang Yi Huang Hybrid hydrogels containing gradients in gold nanoparticles for localized delivery of mesenchymal stem cells and enhanced nerve tissues remodeling in vivo Materials Today Bio Hydrogel Gold nanoparticles Mesenchymal stem cells Peripheral nerve injury Neural regeneration |
| title | Hybrid hydrogels containing gradients in gold nanoparticles for localized delivery of mesenchymal stem cells and enhanced nerve tissues remodeling in vivo |
| title_full | Hybrid hydrogels containing gradients in gold nanoparticles for localized delivery of mesenchymal stem cells and enhanced nerve tissues remodeling in vivo |
| title_fullStr | Hybrid hydrogels containing gradients in gold nanoparticles for localized delivery of mesenchymal stem cells and enhanced nerve tissues remodeling in vivo |
| title_full_unstemmed | Hybrid hydrogels containing gradients in gold nanoparticles for localized delivery of mesenchymal stem cells and enhanced nerve tissues remodeling in vivo |
| title_short | Hybrid hydrogels containing gradients in gold nanoparticles for localized delivery of mesenchymal stem cells and enhanced nerve tissues remodeling in vivo |
| title_sort | hybrid hydrogels containing gradients in gold nanoparticles for localized delivery of mesenchymal stem cells and enhanced nerve tissues remodeling in vivo |
| topic | Hydrogel Gold nanoparticles Mesenchymal stem cells Peripheral nerve injury Neural regeneration |
| url | http://www.sciencedirect.com/science/article/pii/S2590006424004721 |
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