CYP4F18-Deficient Neutrophils Exhibit Increased Chemotaxis to Complement Component C5a
CYP4Fs were first identified as enzymes that catalyze hydroxylation of leukotriene B4 (LTB4). CYP4F18 has an unusual expression in neutrophils and was predicted to play a role in regulating LTB4-dependent inflammation. We compared chemotaxis of wild-type and Cyp4f18 knockout neutrophils using an in...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2015/250456 |
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| _version_ | 1849305528700764160 |
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| author | Rachel Vaivoda Christine Vaine Cassandra Boerstler Kristy Galloway Peter Christmas |
| author_facet | Rachel Vaivoda Christine Vaine Cassandra Boerstler Kristy Galloway Peter Christmas |
| author_sort | Rachel Vaivoda |
| collection | DOAJ |
| description | CYP4Fs were first identified as enzymes that catalyze hydroxylation of leukotriene B4 (LTB4). CYP4F18 has an unusual expression in neutrophils and was predicted to play a role in regulating LTB4-dependent inflammation. We compared chemotaxis of wild-type and Cyp4f18 knockout neutrophils using an in vitro assay. There was no significant difference in the chemotactic response to LTB4, but the response to complement component C5a increased 1.9–2.25-fold in knockout cells compared to wild-type (P < 0.01). This increase was still observed when neutrophils were treated with inhibitors of eicosanoid synthesis. There were no changes in expression of other CYP4 enzymes in knockout neutrophils that might compensate for loss of CYP4F18 or lead to differences in activity. A mouse model of dextran sodium sulfate colitis was used to investigate the consequences of increased C5a-dependent chemotaxis in vivo, but there was no significant difference in weight loss, disease activity, or colonic tissue myeloperoxidase between wild-type and Cyp4f18 knockout mice. This study demonstrates the limitations of inferring CYP4F function based on an ability to use LTB4 as a substrate, points to expanding roles for CYP4F enzymes in immune regulation, and underscores the in vivo challenges of CYP knockout studies. |
| format | Article |
| id | doaj-art-06b27921a1d04f75a940fc788b70c704 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-06b27921a1d04f75a940fc788b70c7042025-08-20T03:55:24ZengWileyJournal of Immunology Research2314-88612314-71562015-01-01201510.1155/2015/250456250456CYP4F18-Deficient Neutrophils Exhibit Increased Chemotaxis to Complement Component C5aRachel Vaivoda0Christine Vaine1Cassandra Boerstler2Kristy Galloway3Peter Christmas4Nephrology Division, Department of Medicine, Massachusetts General Hospital, Charlestown, MA 02129, USANephrology Division, Department of Medicine, Massachusetts General Hospital, Charlestown, MA 02129, USADepartment of Biology, Radford University, Radford, VA 24142, USADepartment of Biology, Radford University, Radford, VA 24142, USANephrology Division, Department of Medicine, Massachusetts General Hospital, Charlestown, MA 02129, USACYP4Fs were first identified as enzymes that catalyze hydroxylation of leukotriene B4 (LTB4). CYP4F18 has an unusual expression in neutrophils and was predicted to play a role in regulating LTB4-dependent inflammation. We compared chemotaxis of wild-type and Cyp4f18 knockout neutrophils using an in vitro assay. There was no significant difference in the chemotactic response to LTB4, but the response to complement component C5a increased 1.9–2.25-fold in knockout cells compared to wild-type (P < 0.01). This increase was still observed when neutrophils were treated with inhibitors of eicosanoid synthesis. There were no changes in expression of other CYP4 enzymes in knockout neutrophils that might compensate for loss of CYP4F18 or lead to differences in activity. A mouse model of dextran sodium sulfate colitis was used to investigate the consequences of increased C5a-dependent chemotaxis in vivo, but there was no significant difference in weight loss, disease activity, or colonic tissue myeloperoxidase between wild-type and Cyp4f18 knockout mice. This study demonstrates the limitations of inferring CYP4F function based on an ability to use LTB4 as a substrate, points to expanding roles for CYP4F enzymes in immune regulation, and underscores the in vivo challenges of CYP knockout studies.http://dx.doi.org/10.1155/2015/250456 |
| spellingShingle | Rachel Vaivoda Christine Vaine Cassandra Boerstler Kristy Galloway Peter Christmas CYP4F18-Deficient Neutrophils Exhibit Increased Chemotaxis to Complement Component C5a Journal of Immunology Research |
| title | CYP4F18-Deficient Neutrophils Exhibit Increased Chemotaxis to Complement Component C5a |
| title_full | CYP4F18-Deficient Neutrophils Exhibit Increased Chemotaxis to Complement Component C5a |
| title_fullStr | CYP4F18-Deficient Neutrophils Exhibit Increased Chemotaxis to Complement Component C5a |
| title_full_unstemmed | CYP4F18-Deficient Neutrophils Exhibit Increased Chemotaxis to Complement Component C5a |
| title_short | CYP4F18-Deficient Neutrophils Exhibit Increased Chemotaxis to Complement Component C5a |
| title_sort | cyp4f18 deficient neutrophils exhibit increased chemotaxis to complement component c5a |
| url | http://dx.doi.org/10.1155/2015/250456 |
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