Detection and molecular characterization of mastadenoviruses in calves with respiratory system infection
<b>Aim: </b>Bovine adenovirus (BAdV) is one of the viral agents that causes respiratory illness in cattle, along with numerous other viral agents. Although the prevalence of BAdV has been determined by serological studies conducted in our country, there are limited data on the detection...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Selcuk University Press
2022-06-01
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| Series: | Eurasian Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1389 |
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| Summary: | <b>Aim: </b>Bovine adenovirus (BAdV) is one of the viral agents that causes respiratory
illness in cattle, along with numerous other viral agents. Although the prevalence
of BAdV has been determined by serological studies conducted in our
country, there are limited data on the detection of BAdVs in respiratory system
samples, and there is no study on their molecular characterization. The aim of
this study was to detect and characterize BAdVs of the Mastadenovirus genus.<p>
<b>Materials and Methods: </b>In this study, a total of 64 nasal swabs and one
lung sample from 65 cattle of different ages with respiratory system disease
symptoms were used. After the extraction of viral DNA, they were tested by
PCR using primers targeting the hexon gene region and the expected size of
amplicons were sequenced.<p>
<b>Results:</b> According to the PCR results, three samples were found positive and
the positivity rate was detected as 4.6%. Out of positive samples, two were
able to be sequenced and one clustered in the BAdV-2 serotype while the other
was grouped in the BAdV-3 serotype.<p>
<b>Conclusion:</b> In this study, it was revealed that BAdVs are contributing agents
in respiratory system infection in our country. Also, this is the first study reporting
the molecular characterization of BAdV-2 and BAdV-3 in calves with
respiratory system infection in Turkey. |
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| ISSN: | 1309-6958 2146-1953 |