Maspin, Syndecan-1, and Ki-67 in the Odontogenic Keratocyst: An Immunohistochemical Analysis
The odontogenic keratocyst (OKC) is a controversial lesion that was reclassified as a tumor with the name “keratocystic odontogenic tumor” in 2005. The reclassification was revoked recently in 2017, with a conclusion on the need for further studies on the subject. In this study, the expressions of a...
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Wiley
2020-01-01
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| Series: | International Journal of Dentistry |
| Online Access: | http://dx.doi.org/10.1155/2020/7041520 |
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| author | Huda M. Hammad Omar M. Nagrash Rima A. Safadi |
| author_facet | Huda M. Hammad Omar M. Nagrash Rima A. Safadi |
| author_sort | Huda M. Hammad |
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| description | The odontogenic keratocyst (OKC) is a controversial lesion that was reclassified as a tumor with the name “keratocystic odontogenic tumor” in 2005. The reclassification was revoked recently in 2017, with a conclusion on the need for further studies on the subject. In this study, the expressions of an important regulatory protein (maspin), an important integral membrane proteoglycan (syndecan-1), and a universal proliferation marker (Ki-67) in the epithelium of the OKC were investigated in comparison with the dentigerous cyst (DC) and ameloblastoma (AB). Twenty-six OKCs, eleven DCs, and ten conventional ABs were immunohistochemically stained for maspin, syndecan-1, and Ki-67. ImageJ was used to analyze the positivity of maspin and syndecan-1. The Ki-67 score was calculated as the percentage of positive nuclei in 5 high power fields. Analysis of variance (ANOVA) test and Student t-test were used as appropriate. Lower expressions of maspin were noted in OKC and DC compared to those in AB, and lower expressions of syndecan-1 were noted in OKC and AB compared to those in DC. The differences, however, did not reach statistical significance (ANOVA and t-test: P>0.05). The Ki-67 score was significantly higher in OKC than in DC (t-test: P<0.05), and not significantly different from AB (t-test: P>0.05). In conclusion, expressions of maspin and syndecan-1 are not strongly representative of differences in behavior between OKC, AB, and DC. However, the expression of Ki-67 indicates comparable proliferative activities of OKC and AB, which are higher than that of DC. Further investigation on the biologic behavior of OKC is still recommended to arrive at more specific conclusions regarding its classification. |
| format | Article |
| id | doaj-art-069df74d711b45e4ad202bb59574e5d7 |
| institution | Kabale University |
| issn | 1687-8728 1687-8736 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Dentistry |
| spelling | doaj-art-069df74d711b45e4ad202bb59574e5d72025-08-20T03:55:28ZengWileyInternational Journal of Dentistry1687-87281687-87362020-01-01202010.1155/2020/70415207041520Maspin, Syndecan-1, and Ki-67 in the Odontogenic Keratocyst: An Immunohistochemical AnalysisHuda M. Hammad0Omar M. Nagrash1Rima A. Safadi2Department of Oral Medicine and Oral Surgery, Faculty of Dentistry, Jordan University of Science and Technology, P. O. Box 3030, Irbid 22110, JordanAl-Ramtha Governmental Hospital, Ministry of Health, P. O. Box 132, Al-Ramtha 21410, JordanDepartment of Oral Medicine and Oral Surgery, Faculty of Dentistry, Jordan University of Science and Technology, P. O. Box 3030, Irbid 22110, JordanThe odontogenic keratocyst (OKC) is a controversial lesion that was reclassified as a tumor with the name “keratocystic odontogenic tumor” in 2005. The reclassification was revoked recently in 2017, with a conclusion on the need for further studies on the subject. In this study, the expressions of an important regulatory protein (maspin), an important integral membrane proteoglycan (syndecan-1), and a universal proliferation marker (Ki-67) in the epithelium of the OKC were investigated in comparison with the dentigerous cyst (DC) and ameloblastoma (AB). Twenty-six OKCs, eleven DCs, and ten conventional ABs were immunohistochemically stained for maspin, syndecan-1, and Ki-67. ImageJ was used to analyze the positivity of maspin and syndecan-1. The Ki-67 score was calculated as the percentage of positive nuclei in 5 high power fields. Analysis of variance (ANOVA) test and Student t-test were used as appropriate. Lower expressions of maspin were noted in OKC and DC compared to those in AB, and lower expressions of syndecan-1 were noted in OKC and AB compared to those in DC. The differences, however, did not reach statistical significance (ANOVA and t-test: P>0.05). The Ki-67 score was significantly higher in OKC than in DC (t-test: P<0.05), and not significantly different from AB (t-test: P>0.05). In conclusion, expressions of maspin and syndecan-1 are not strongly representative of differences in behavior between OKC, AB, and DC. However, the expression of Ki-67 indicates comparable proliferative activities of OKC and AB, which are higher than that of DC. Further investigation on the biologic behavior of OKC is still recommended to arrive at more specific conclusions regarding its classification.http://dx.doi.org/10.1155/2020/7041520 |
| spellingShingle | Huda M. Hammad Omar M. Nagrash Rima A. Safadi Maspin, Syndecan-1, and Ki-67 in the Odontogenic Keratocyst: An Immunohistochemical Analysis International Journal of Dentistry |
| title | Maspin, Syndecan-1, and Ki-67 in the Odontogenic Keratocyst: An Immunohistochemical Analysis |
| title_full | Maspin, Syndecan-1, and Ki-67 in the Odontogenic Keratocyst: An Immunohistochemical Analysis |
| title_fullStr | Maspin, Syndecan-1, and Ki-67 in the Odontogenic Keratocyst: An Immunohistochemical Analysis |
| title_full_unstemmed | Maspin, Syndecan-1, and Ki-67 in the Odontogenic Keratocyst: An Immunohistochemical Analysis |
| title_short | Maspin, Syndecan-1, and Ki-67 in the Odontogenic Keratocyst: An Immunohistochemical Analysis |
| title_sort | maspin syndecan 1 and ki 67 in the odontogenic keratocyst an immunohistochemical analysis |
| url | http://dx.doi.org/10.1155/2020/7041520 |
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