In Vitro Regeneration of Tea (Camellia sinensis (L). O. Kuntze) By Somatic Embryogenesis from Immature Cotyledon Tissues
Tea (Camellia sinensis) is the world's most popular beverage plant, as well as an important plantation crop with high commercial value. It has been maintained for centuries through conventional vegetative propagation. Tea clonal propagation in vitro has the advantage of producing a large number...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Hasan Eleroğlu
2021-12-01
|
| Series: | Turkish Journal of Agriculture: Food Science and Technology |
| Subjects: | |
| Online Access: | http://www.agrifoodscience.com/index.php/TURJAF/article/view/4940 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849305527515873280 |
|---|---|
| author | Emine Yurteri Mücahit Salih Can Fatih Seyis Haydar Kuplemez |
| author_facet | Emine Yurteri Mücahit Salih Can Fatih Seyis Haydar Kuplemez |
| author_sort | Emine Yurteri |
| collection | DOAJ |
| description | Tea (Camellia sinensis) is the world's most popular beverage plant, as well as an important plantation crop with high commercial value. It has been maintained for centuries through conventional vegetative propagation. Tea clonal propagation in vitro has the advantage of producing a large number of elite plants. If an efficient in vitro regeneration technology is available, this technique could be exploited for selection of tea plants for desired trait. The selected plants could be later on multiplied through in vitro or ex vitro techniques. The study aimed to induced somatic embryogenesis from immature embryo explants to genetic variaton. Different concentrations of phenylboronic acid with benzyladenine and phenylboronic acid with kinetin were tested in MS medium with 30 g/L sucrose and 8 g/L agar. MS medium without any plant growth regulators was used as control group. Considering the embryo survival rate, 1.5 mg/ L-1 phenylboronic acid + 1 mg/ L-1 kinetin produced highest result as 87.3% while lowest was in control group as 36.7%. The highest plant regeneration rate was found in 1,5 mg/ L-1 phenylboronic acid + 1 mg/ L-1 kinetin and 1.5 mg/ L-1 phenylboronic acid + 1 mg/ L-1 benzyladenine medium respectively as 58.3% and 55.6%. Kinetin treatment with increasing phenylboronic acid concentrations gave the best results in terms of somatic embryo survival rate. Also, kinetin treatment produced better results when compared to benzyladenine concentrations. |
| format | Article |
| id | doaj-art-068b49ee689e464cb7c9d831c67c2634 |
| institution | Kabale University |
| issn | 2148-127X |
| language | English |
| publishDate | 2021-12-01 |
| publisher | Hasan Eleroğlu |
| record_format | Article |
| series | Turkish Journal of Agriculture: Food Science and Technology |
| spelling | doaj-art-068b49ee689e464cb7c9d831c67c26342025-08-20T03:55:27ZengHasan EleroğluTurkish Journal of Agriculture: Food Science and Technology2148-127X2021-12-019sp2587259010.24925/turjaf.v9isp.2587-2590.49402334In Vitro Regeneration of Tea (Camellia sinensis (L). O. Kuntze) By Somatic Embryogenesis from Immature Cotyledon TissuesEmine Yurteri0Mücahit Salih Can1Fatih Seyis2Haydar Kuplemez3Field Crops Department, Faculty of Agriculture, Recep Tayyip Erdogan University, 53100 RizeField Crops Department, Faculty of Agriculture, Recep Tayyip Erdogan University, 53100 RizeField Crops Department, Faculty of Agriculture, Recep Tayyip Erdogan University, 53100 RizeField Crops Department, Faculty of Agriculture, Recep Tayyip Erdogan University, 53100 RizeTea (Camellia sinensis) is the world's most popular beverage plant, as well as an important plantation crop with high commercial value. It has been maintained for centuries through conventional vegetative propagation. Tea clonal propagation in vitro has the advantage of producing a large number of elite plants. If an efficient in vitro regeneration technology is available, this technique could be exploited for selection of tea plants for desired trait. The selected plants could be later on multiplied through in vitro or ex vitro techniques. The study aimed to induced somatic embryogenesis from immature embryo explants to genetic variaton. Different concentrations of phenylboronic acid with benzyladenine and phenylboronic acid with kinetin were tested in MS medium with 30 g/L sucrose and 8 g/L agar. MS medium without any plant growth regulators was used as control group. Considering the embryo survival rate, 1.5 mg/ L-1 phenylboronic acid + 1 mg/ L-1 kinetin produced highest result as 87.3% while lowest was in control group as 36.7%. The highest plant regeneration rate was found in 1,5 mg/ L-1 phenylboronic acid + 1 mg/ L-1 kinetin and 1.5 mg/ L-1 phenylboronic acid + 1 mg/ L-1 benzyladenine medium respectively as 58.3% and 55.6%. Kinetin treatment with increasing phenylboronic acid concentrations gave the best results in terms of somatic embryo survival rate. Also, kinetin treatment produced better results when compared to benzyladenine concentrations.http://www.agrifoodscience.com/index.php/TURJAF/article/view/4940camellia sinensistissue culturesomatic embryogenesisimmature cotyledonimmortal flower |
| spellingShingle | Emine Yurteri Mücahit Salih Can Fatih Seyis Haydar Kuplemez In Vitro Regeneration of Tea (Camellia sinensis (L). O. Kuntze) By Somatic Embryogenesis from Immature Cotyledon Tissues Turkish Journal of Agriculture: Food Science and Technology camellia sinensis tissue culture somatic embryogenesis immature cotyledon immortal flower |
| title | In Vitro Regeneration of Tea (Camellia sinensis (L). O. Kuntze) By Somatic Embryogenesis from Immature Cotyledon Tissues |
| title_full | In Vitro Regeneration of Tea (Camellia sinensis (L). O. Kuntze) By Somatic Embryogenesis from Immature Cotyledon Tissues |
| title_fullStr | In Vitro Regeneration of Tea (Camellia sinensis (L). O. Kuntze) By Somatic Embryogenesis from Immature Cotyledon Tissues |
| title_full_unstemmed | In Vitro Regeneration of Tea (Camellia sinensis (L). O. Kuntze) By Somatic Embryogenesis from Immature Cotyledon Tissues |
| title_short | In Vitro Regeneration of Tea (Camellia sinensis (L). O. Kuntze) By Somatic Embryogenesis from Immature Cotyledon Tissues |
| title_sort | in vitro regeneration of tea camellia sinensis l o kuntze by somatic embryogenesis from immature cotyledon tissues |
| topic | camellia sinensis tissue culture somatic embryogenesis immature cotyledon immortal flower |
| url | http://www.agrifoodscience.com/index.php/TURJAF/article/view/4940 |
| work_keys_str_mv | AT emineyurteri invitroregenerationofteacamelliasinensislokuntzebysomaticembryogenesisfromimmaturecotyledontissues AT mucahitsalihcan invitroregenerationofteacamelliasinensislokuntzebysomaticembryogenesisfromimmaturecotyledontissues AT fatihseyis invitroregenerationofteacamelliasinensislokuntzebysomaticembryogenesisfromimmaturecotyledontissues AT haydarkuplemez invitroregenerationofteacamelliasinensislokuntzebysomaticembryogenesisfromimmaturecotyledontissues |