Alkali burn injury model of meibomian gland dysfunction in mice
AIM: To establish a stable, short-time, low-cost and reliable murine model of meibomian gland dysfunction (MGD). METHODS: A filter paper sheet soaked in 1.0 mol/L sodium hydroxide (NaOH) solution was used to touch the eyelid margin of C57BL/6J mice for 10s to establish the model. The other eye was l...
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Press of International Journal of Ophthalmology (IJO PRESS)
2024-12-01
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| Series: | International Journal of Ophthalmology |
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| Online Access: | http://ies.ijo.cn/en_publish/2024/12/20241202.pdf |
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| author | Yong Li Ya-Qiong Yang Yong Lin Ke Yan Yu-Fei Lyu Zhao-Qiang Zhang Cai-Hong Huang Jiao-Yue Hu Zu-Guo Liu |
| author_facet | Yong Li Ya-Qiong Yang Yong Lin Ke Yan Yu-Fei Lyu Zhao-Qiang Zhang Cai-Hong Huang Jiao-Yue Hu Zu-Guo Liu |
| author_sort | Yong Li |
| collection | DOAJ |
| description | AIM: To establish a stable, short-time, low-cost and reliable murine model of meibomian gland dysfunction (MGD). METHODS: A filter paper sheet soaked in 1.0 mol/L sodium hydroxide (NaOH) solution was used to touch the eyelid margin of C57BL/6J mice for 10s to establish the model. The other eye was left untreated as a control group. Eyelid margin morphological changes and the meibomian glands (MGs) were observed by slit lamp microscopy on days 5 and 10 post-burn. Hematoxylin-eosin (HE) staining and Oil red O staining were adopted in detecting the changes in MGs morphology and lipid deposition. Real-time polymerase chain reaction, Western blot, immunofluorescence staining and immunohistochemical staining were used to detect interleukin (IL)-6, IL-1β, IL-18, tumor necroses factor (TNF)-α, interferon (IFN)-γ, nicotinamide adenine dinucleotide phosphate (NADPH) oxidase 4 (NOX4), 3-nitroturosine (3-NT), 4-hydroxynonenal (4-HNE) and cytokeratin 10 (K10) expression changes in MGs. RESULTS: MGs showed plugging of orifice, glandular deficiency, abnormal acinar morphology, ductal dilatation, and lipid deposition after alkali burn. The expressions of IL-6, IL-18, IL-1β, IFN-γ, and TNF-α indicators of inflammation and oxidative stress in MGs tissues were significantly increased. Abnormal keratinization increased in the MG duct. CONCLUSION: A murine model of MGD is established by alkali burn of the eyelid margin that matches the clinical presentation of MGD providing a stable, short-time, low-cost, and reliable MGD model. The new method suggests efficient avenues for future research. |
| format | Article |
| id | doaj-art-05cb4e9c6b4641e782b02d94d9ca79d9 |
| institution | Kabale University |
| issn | 2222-3959 2227-4898 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | International Journal of Ophthalmology |
| spelling | doaj-art-05cb4e9c6b4641e782b02d94d9ca79d92024-11-19T03:13:35ZengPress of International Journal of Ophthalmology (IJO PRESS)International Journal of Ophthalmology2222-39592227-48982024-12-0117122158216610.18240/ijo.2024.12.0220241202Alkali burn injury model of meibomian gland dysfunction in miceYong Li0Ya-Qiong Yang1Yong Lin2Ke Yan3Yu-Fei Lyu4Zhao-Qiang Zhang5Cai-Hong Huang6Jiao-Yue Hu7Zu-Guo Liu8Zu-Guo Liu. Key Laboratory of Ophthalmology, University of South China, Hengyang 421000, Hunan Province, China. zuguoliu@xmu.edu.cnXiamen University Affiliated Xiamen Eye Center, Eye Institute of Xiamen University, School of Medicine, Xiamen University, Xiamen 3611002, Fujian Province, China; Fujian Provincial Key Laboratory of Ophthalmology and Visual Science, Fujian Engineering and Research Center of Eye Regenerative Medicine, Xiamen 3611002, Fujian Province, ChinaDepartment of Traditional Chinese Medicine, School of Medicine, Xiamen University, Xiamen 361100, Fujian Province, ChinaDepartment of Ophthalmology, the First Affiliated Hospital of University of South China, Hengyang 421001, Hunan Province, ChinaDepartment of Traditional Chinese Medicine, School of Medicine, Xiamen University, Xiamen 361100, Fujian Province, ChinaXiamen University Affiliated Xiamen Eye Center, Eye Institute of Xiamen University, School of Medicine, Xiamen University, Xiamen 3611002, Fujian Province, China; Fujian Provincial Key Laboratory of Ophthalmology and Visual Science, Fujian Engineering and Research Center of Eye Regenerative Medicine, Xiamen 3611002, Fujian Province, ChinaXiamen University Affiliated Xiamen Eye Center, Eye Institute of Xiamen University, School of Medicine, Xiamen University, Xiamen 3611002, Fujian Province, China; Fujian Provincial Key Laboratory of Ophthalmology and Visual Science, Fujian Engineering and Research Center of Eye Regenerative Medicine, Xiamen 3611002, Fujian Province, ChinaXiamen University Affiliated Xiamen Eye Center, Eye Institute of Xiamen University, School of Medicine, Xiamen University, Xiamen 3611002, Fujian Province, China; Fujian Provincial Key Laboratory of Ophthalmology and Visual Science, Fujian Engineering and Research Center of Eye Regenerative Medicine, Xiamen 3611002, Fujian Province, ChinaXiamen University Affiliated Xiamen Eye Center, Eye Institute of Xiamen University, School of Medicine, Xiamen University, Xiamen 3611002, Fujian Province, China; The Second Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang 421000, Hunan Province, China; Fujian Provincial Key Laboratory of Ophthalmology and Visual Science, Fujian Engineering and Research Center of Eye Regenerative Medicine, Xiamen 3611002, Fujian Province, China; Department of Traditional Chinese Medicine, School of Medicine, Xiamen University, Xiamen 361100, Fujian Province, ChinaAIM: To establish a stable, short-time, low-cost and reliable murine model of meibomian gland dysfunction (MGD). METHODS: A filter paper sheet soaked in 1.0 mol/L sodium hydroxide (NaOH) solution was used to touch the eyelid margin of C57BL/6J mice for 10s to establish the model. The other eye was left untreated as a control group. Eyelid margin morphological changes and the meibomian glands (MGs) were observed by slit lamp microscopy on days 5 and 10 post-burn. Hematoxylin-eosin (HE) staining and Oil red O staining were adopted in detecting the changes in MGs morphology and lipid deposition. Real-time polymerase chain reaction, Western blot, immunofluorescence staining and immunohistochemical staining were used to detect interleukin (IL)-6, IL-1β, IL-18, tumor necroses factor (TNF)-α, interferon (IFN)-γ, nicotinamide adenine dinucleotide phosphate (NADPH) oxidase 4 (NOX4), 3-nitroturosine (3-NT), 4-hydroxynonenal (4-HNE) and cytokeratin 10 (K10) expression changes in MGs. RESULTS: MGs showed plugging of orifice, glandular deficiency, abnormal acinar morphology, ductal dilatation, and lipid deposition after alkali burn. The expressions of IL-6, IL-18, IL-1β, IFN-γ, and TNF-α indicators of inflammation and oxidative stress in MGs tissues were significantly increased. Abnormal keratinization increased in the MG duct. CONCLUSION: A murine model of MGD is established by alkali burn of the eyelid margin that matches the clinical presentation of MGD providing a stable, short-time, low-cost, and reliable MGD model. The new method suggests efficient avenues for future research.http://ies.ijo.cn/en_publish/2024/12/20241202.pdfalkali burnmeibomian gland dysfunctionanimal modeloxidative stress |
| spellingShingle | Yong Li Ya-Qiong Yang Yong Lin Ke Yan Yu-Fei Lyu Zhao-Qiang Zhang Cai-Hong Huang Jiao-Yue Hu Zu-Guo Liu Alkali burn injury model of meibomian gland dysfunction in mice International Journal of Ophthalmology alkali burn meibomian gland dysfunction animal model oxidative stress |
| title | Alkali burn injury model of meibomian gland dysfunction in mice |
| title_full | Alkali burn injury model of meibomian gland dysfunction in mice |
| title_fullStr | Alkali burn injury model of meibomian gland dysfunction in mice |
| title_full_unstemmed | Alkali burn injury model of meibomian gland dysfunction in mice |
| title_short | Alkali burn injury model of meibomian gland dysfunction in mice |
| title_sort | alkali burn injury model of meibomian gland dysfunction in mice |
| topic | alkali burn meibomian gland dysfunction animal model oxidative stress |
| url | http://ies.ijo.cn/en_publish/2024/12/20241202.pdf |
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