Biogenic silver nanoparticles from Simarouba glauca DC leaf extract: Synthesis, characterization, and anticancer efficacy in lung cancer cells with protective effects in Caenorhabditis elegans
This study explores the synthesis, characterization, and biomedical applications of silver nanoparticles (AgNPs) synthesized using Simarouba glauca leaf extract. The biogenic AgNPs were characterized through UV–visible spectroscopy, X-ray diffraction (XRD), particle size analysis, zeta potential ana...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2024-12-01
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| Series: | Nano TransMed |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2790676024000232 |
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| author | Santosh Mallikarjun Bhavi Akshata Choudhari Padti Bothe Thokchom Sapam Riches Singh Shivanand S. Bhat Sukesh Kumar Bajire Rajesh P. Shastry B.S. Srinath Sushma Subraya Gummani B.P. Harini Ramesh Babu Yarajarla |
| author_facet | Santosh Mallikarjun Bhavi Akshata Choudhari Padti Bothe Thokchom Sapam Riches Singh Shivanand S. Bhat Sukesh Kumar Bajire Rajesh P. Shastry B.S. Srinath Sushma Subraya Gummani B.P. Harini Ramesh Babu Yarajarla |
| author_sort | Santosh Mallikarjun Bhavi |
| collection | DOAJ |
| description | This study explores the synthesis, characterization, and biomedical applications of silver nanoparticles (AgNPs) synthesized using Simarouba glauca leaf extract. The biogenic AgNPs were characterized through UV–visible spectroscopy, X-ray diffraction (XRD), particle size analysis, zeta potential analysis, energy dispersive X-ray spectroscopy (EDX), Fourier-transform infrared spectroscopy (FTIR), atomic force microscopy (AFM), and high-resolution transmission electron microscopy (HR-TEM). The nanoparticles exhibited a distinct absorbance peak at 413.5 nm, confirming their synthesis and surface plasmon resonance. XRD and HR-TEM analyses revealed a face-centered cubic structure with an average size of 12.45 nm, while AFM indicated a mean particle size of 18.34 nm. The particles demonstrated moderate stability with a zeta potential of −41.4 mV and exhibited various elemental compositions as confirmed by EDX. In the biomedical evaluation, the anticancer potential of SG-AgNPs was tested on L-132 and A549 cell lines using MTT, live/dead, and DNA damage assays. Results showed dose-dependent cytotoxicity, with significant effects observed at higher concentrations, particularly against A549 cells. Additionally, the protective effects of SG-AgNPs were assessed in Caenorhabditis elegans using survival and paralysis assays. The nanoparticles significantly improved survival rates and reduced paralysis in worms infected with Pseudomonas aeruginosa PAO1 and Staphylococcus aureus. These findings highlight the potential of biogenic SG-AgNPs as effective agents with both anticancer and antimicrobial properties. Future research should focus on elucidating the mechanisms underlying these effects and optimizing their therapeutic applications. |
| format | Article |
| id | doaj-art-0592e9d8bc964ac6b7f8b7e2d380bd4e |
| institution | Kabale University |
| issn | 2790-6760 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Nano TransMed |
| spelling | doaj-art-0592e9d8bc964ac6b7f8b7e2d380bd4e2024-12-18T08:55:21ZengKeAi Communications Co., Ltd.Nano TransMed2790-67602024-12-013100052Biogenic silver nanoparticles from Simarouba glauca DC leaf extract: Synthesis, characterization, and anticancer efficacy in lung cancer cells with protective effects in Caenorhabditis elegansSantosh Mallikarjun Bhavi0Akshata Choudhari Padti1Bothe Thokchom2Sapam Riches Singh3Shivanand S. Bhat4Sukesh Kumar Bajire5Rajesh P. Shastry6B.S. Srinath7Sushma Subraya Gummani8B.P. Harini9Ramesh Babu Yarajarla10Drosophila and Nanoscience Research Laboratory, Department of Applied Genetics, Karnatak University, Dharwad, Karnataka 580003, IndiaDrosophila and Nanoscience Research Laboratory, Department of Applied Genetics, Karnatak University, Dharwad, Karnataka 580003, IndiaDrosophila and Nanoscience Research Laboratory, Department of Applied Genetics, Karnatak University, Dharwad, Karnataka 580003, IndiaDrosophila and Nanoscience Research Laboratory, Department of Applied Genetics, Karnatak University, Dharwad, Karnataka 580003, IndiaDepartment of Botany, Smt. Indira Gandhi Government First Grade Women’s College, Sagar, Karnataka 577401, IndiaDivision of Microbiology and Biotechnology, Yenepoya Research Centre, Yenepoya (Deemed to be University), Deralakatte, Mangalore 575018, IndiaDivision of Microbiology and Biotechnology, Yenepoya Research Centre, Yenepoya (Deemed to be University), Deralakatte, Mangalore 575018, IndiaDepartment of Microbiology and Biotechnology, Bangalore University, Bangalore, Karnataka 560056, IndiaDrosophila and Nanoscience Research Laboratory, Department of Applied Genetics, Karnatak University, Dharwad, Karnataka 580003, IndiaDepartment of Zoology, Bangalore University, Bangalore, Karnataka 560056, IndiaDrosophila and Nanoscience Research Laboratory, Department of Applied Genetics, Karnatak University, Dharwad, Karnataka 580003, India; Corresponding author.This study explores the synthesis, characterization, and biomedical applications of silver nanoparticles (AgNPs) synthesized using Simarouba glauca leaf extract. The biogenic AgNPs were characterized through UV–visible spectroscopy, X-ray diffraction (XRD), particle size analysis, zeta potential analysis, energy dispersive X-ray spectroscopy (EDX), Fourier-transform infrared spectroscopy (FTIR), atomic force microscopy (AFM), and high-resolution transmission electron microscopy (HR-TEM). The nanoparticles exhibited a distinct absorbance peak at 413.5 nm, confirming their synthesis and surface plasmon resonance. XRD and HR-TEM analyses revealed a face-centered cubic structure with an average size of 12.45 nm, while AFM indicated a mean particle size of 18.34 nm. The particles demonstrated moderate stability with a zeta potential of −41.4 mV and exhibited various elemental compositions as confirmed by EDX. In the biomedical evaluation, the anticancer potential of SG-AgNPs was tested on L-132 and A549 cell lines using MTT, live/dead, and DNA damage assays. Results showed dose-dependent cytotoxicity, with significant effects observed at higher concentrations, particularly against A549 cells. Additionally, the protective effects of SG-AgNPs were assessed in Caenorhabditis elegans using survival and paralysis assays. The nanoparticles significantly improved survival rates and reduced paralysis in worms infected with Pseudomonas aeruginosa PAO1 and Staphylococcus aureus. These findings highlight the potential of biogenic SG-AgNPs as effective agents with both anticancer and antimicrobial properties. Future research should focus on elucidating the mechanisms underlying these effects and optimizing their therapeutic applications.http://www.sciencedirect.com/science/article/pii/S2790676024000232Silver nanoparticlesSimarouba glaucaCytotoxicityCaenorhabditis elegansAntimicrobial properties |
| spellingShingle | Santosh Mallikarjun Bhavi Akshata Choudhari Padti Bothe Thokchom Sapam Riches Singh Shivanand S. Bhat Sukesh Kumar Bajire Rajesh P. Shastry B.S. Srinath Sushma Subraya Gummani B.P. Harini Ramesh Babu Yarajarla Biogenic silver nanoparticles from Simarouba glauca DC leaf extract: Synthesis, characterization, and anticancer efficacy in lung cancer cells with protective effects in Caenorhabditis elegans Nano TransMed Silver nanoparticles Simarouba glauca Cytotoxicity Caenorhabditis elegans Antimicrobial properties |
| title | Biogenic silver nanoparticles from Simarouba glauca DC leaf extract: Synthesis, characterization, and anticancer efficacy in lung cancer cells with protective effects in Caenorhabditis elegans |
| title_full | Biogenic silver nanoparticles from Simarouba glauca DC leaf extract: Synthesis, characterization, and anticancer efficacy in lung cancer cells with protective effects in Caenorhabditis elegans |
| title_fullStr | Biogenic silver nanoparticles from Simarouba glauca DC leaf extract: Synthesis, characterization, and anticancer efficacy in lung cancer cells with protective effects in Caenorhabditis elegans |
| title_full_unstemmed | Biogenic silver nanoparticles from Simarouba glauca DC leaf extract: Synthesis, characterization, and anticancer efficacy in lung cancer cells with protective effects in Caenorhabditis elegans |
| title_short | Biogenic silver nanoparticles from Simarouba glauca DC leaf extract: Synthesis, characterization, and anticancer efficacy in lung cancer cells with protective effects in Caenorhabditis elegans |
| title_sort | biogenic silver nanoparticles from simarouba glauca dc leaf extract synthesis characterization and anticancer efficacy in lung cancer cells with protective effects in caenorhabditis elegans |
| topic | Silver nanoparticles Simarouba glauca Cytotoxicity Caenorhabditis elegans Antimicrobial properties |
| url | http://www.sciencedirect.com/science/article/pii/S2790676024000232 |
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