Non-stochastic reassembly of a metabolically cohesive gut consortium shaped by N-acetyl-lactosamine-enriched fibers
Diet is one of the main factors shaping the human microbiome, yet our understanding of how specific dietary components influence microbial consortia assembly and subsequent stability in response to press disturbances – such as increasing resource availability (feeding rate) – is still incomplete. Th...
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Taylor & Francis Group
2025-12-01
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| Series: | Gut Microbes |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/19490976.2024.2440120 |
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| author | Madison Moore Hunter D. Whittington Rebecca Knickmeyer M. Andrea Azcarate-Peril Jose M. Bruno-Bárcena |
| author_facet | Madison Moore Hunter D. Whittington Rebecca Knickmeyer M. Andrea Azcarate-Peril Jose M. Bruno-Bárcena |
| author_sort | Madison Moore |
| collection | DOAJ |
| description | Diet is one of the main factors shaping the human microbiome, yet our understanding of how specific dietary components influence microbial consortia assembly and subsequent stability in response to press disturbances – such as increasing resource availability (feeding rate) – is still incomplete. This study explores the reproducible re-assembly, metabolic interplay, and compositional stability within microbial consortia derived from pooled stool samples of three healthy infants. Using a single-step packed-bed reactor (PBR) system, we assessed the reassembly and metabolic output of consortia exposed to lactose, glucose, galacto-oligosaccharides (GOS), and humanized GOS (hGOS). Our findings reveal that complex carbohydrates, especially those containing low inclusion (~1.25 gL−1) components present in human milk, such as N-acetyl-lactosamine (LacNAc), promote taxonomic, and metabolic stability under varying feeding rates, as shown by diversity metrics and network analysis. Targeted metabolomics highlighted distinct metabolic responses to different carbohydrates: GOS was linked to increased lactate, lactose to propionate, sucrose to butyrate, and CO2, and the introduction of bile salts with GOS or hGOS resulted in butyrate reduction and increased hydrogen production. This study validates the use of single-step PBRs for reliably studying microbial consortium stability and functionality in response to nutritional press disturbances, offering insights into the dietary modulation of microbial consortia and their ecological dynamics. |
| format | Article |
| id | doaj-art-05624667854f44db9cc4c1e9ea5cf490 |
| institution | Kabale University |
| issn | 1949-0976 1949-0984 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Gut Microbes |
| spelling | doaj-art-05624667854f44db9cc4c1e9ea5cf4902024-12-19T04:38:54ZengTaylor & Francis GroupGut Microbes1949-09761949-09842025-12-0117110.1080/19490976.2024.2440120Non-stochastic reassembly of a metabolically cohesive gut consortium shaped by N-acetyl-lactosamine-enriched fibersMadison Moore0Hunter D. Whittington1Rebecca Knickmeyer2M. Andrea Azcarate-Peril3Jose M. Bruno-Bárcena4Department of Plant and Microbial Biology, North Carolina State University, Raleigh, NC, USADepartment of Plant and Microbial Biology, North Carolina State University, Raleigh, NC, USADepartment of Psychiatry, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USADepartment of Medicine, Division of Gastroenterology and Hepatology, and UNC Microbiome Core, Center for Gastrointestinal Biology and Disease (CGIBD), School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USADepartment of Plant and Microbial Biology, North Carolina State University, Raleigh, NC, USADiet is one of the main factors shaping the human microbiome, yet our understanding of how specific dietary components influence microbial consortia assembly and subsequent stability in response to press disturbances – such as increasing resource availability (feeding rate) – is still incomplete. This study explores the reproducible re-assembly, metabolic interplay, and compositional stability within microbial consortia derived from pooled stool samples of three healthy infants. Using a single-step packed-bed reactor (PBR) system, we assessed the reassembly and metabolic output of consortia exposed to lactose, glucose, galacto-oligosaccharides (GOS), and humanized GOS (hGOS). Our findings reveal that complex carbohydrates, especially those containing low inclusion (~1.25 gL−1) components present in human milk, such as N-acetyl-lactosamine (LacNAc), promote taxonomic, and metabolic stability under varying feeding rates, as shown by diversity metrics and network analysis. Targeted metabolomics highlighted distinct metabolic responses to different carbohydrates: GOS was linked to increased lactate, lactose to propionate, sucrose to butyrate, and CO2, and the introduction of bile salts with GOS or hGOS resulted in butyrate reduction and increased hydrogen production. This study validates the use of single-step PBRs for reliably studying microbial consortium stability and functionality in response to nutritional press disturbances, offering insights into the dietary modulation of microbial consortia and their ecological dynamics.https://www.tandfonline.com/doi/10.1080/19490976.2024.2440120Dietary modulationHMOmicrobial ecologynutritioninfant microbiota |
| spellingShingle | Madison Moore Hunter D. Whittington Rebecca Knickmeyer M. Andrea Azcarate-Peril Jose M. Bruno-Bárcena Non-stochastic reassembly of a metabolically cohesive gut consortium shaped by N-acetyl-lactosamine-enriched fibers Gut Microbes Dietary modulation HMO microbial ecology nutrition infant microbiota |
| title | Non-stochastic reassembly of a metabolically cohesive gut consortium shaped by N-acetyl-lactosamine-enriched fibers |
| title_full | Non-stochastic reassembly of a metabolically cohesive gut consortium shaped by N-acetyl-lactosamine-enriched fibers |
| title_fullStr | Non-stochastic reassembly of a metabolically cohesive gut consortium shaped by N-acetyl-lactosamine-enriched fibers |
| title_full_unstemmed | Non-stochastic reassembly of a metabolically cohesive gut consortium shaped by N-acetyl-lactosamine-enriched fibers |
| title_short | Non-stochastic reassembly of a metabolically cohesive gut consortium shaped by N-acetyl-lactosamine-enriched fibers |
| title_sort | non stochastic reassembly of a metabolically cohesive gut consortium shaped by n acetyl lactosamine enriched fibers |
| topic | Dietary modulation HMO microbial ecology nutrition infant microbiota |
| url | https://www.tandfonline.com/doi/10.1080/19490976.2024.2440120 |
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