Panel containing three serum microRNAs: a promising biomarker for early detection of bladder cancer
BackgroundBladder cancer (BC) is a common tumor worldwide. Screening for BC currently lacks a highly efficient, non-invasive, and inexpensive method. Serum microRNA (miRNA), which is stable and commonly present, has the potential to serve as a novel marker for BC diagnosis.Materials & method...
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Frontiers Media S.A.
2024-12-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2024.1470457/full |
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| author | Zhenjian Ge Zhenjian Ge Zhenjian Ge Zhenjian Ge Shengjie Lin Shengjie Lin Shengjie Lin Shengjie Lin Chong Lu Chong Lu Chong Lu Chong Lu Yong Xia Rongkang Li Rongkang Li Rongkang Li Rongkang Li Xinji Li Xinji Li Xinji Li Xinji Li Chen Sun Chen Sun Chen Sun Chen Sun Zhenyu Wen Zhenyu Wen Zhenyu Wen Zhenyu Wen Wenkang Chen Wenkang Chen Wenkang Chen Wenkang Chen Yingqi Li Yingqi Li Yingqi Li Yingqi Li Mingyang Li Yu Lin Jing Dong Lingzhi Tao Lingzhi Tao Lingzhi Tao Ling Ji Yongqing Lai Yongqing Lai Yongqing Lai |
| author_facet | Zhenjian Ge Zhenjian Ge Zhenjian Ge Zhenjian Ge Shengjie Lin Shengjie Lin Shengjie Lin Shengjie Lin Chong Lu Chong Lu Chong Lu Chong Lu Yong Xia Rongkang Li Rongkang Li Rongkang Li Rongkang Li Xinji Li Xinji Li Xinji Li Xinji Li Chen Sun Chen Sun Chen Sun Chen Sun Zhenyu Wen Zhenyu Wen Zhenyu Wen Zhenyu Wen Wenkang Chen Wenkang Chen Wenkang Chen Wenkang Chen Yingqi Li Yingqi Li Yingqi Li Yingqi Li Mingyang Li Yu Lin Jing Dong Lingzhi Tao Lingzhi Tao Lingzhi Tao Ling Ji Yongqing Lai Yongqing Lai Yongqing Lai |
| author_sort | Zhenjian Ge |
| collection | DOAJ |
| description | BackgroundBladder cancer (BC) is a common tumor worldwide. Screening for BC currently lacks a highly efficient, non-invasive, and inexpensive method. Serum microRNA (miRNA), which is stable and commonly present, has the potential to serve as a novel marker for BC diagnosis.Materials & methodsBased on a study involving 112 BC patients and 112 healthy subjects, we conducted this research in three phases to identify applicable microRNAs (miRNAs) in serum for BC diagnosis using quantitative reverse transcription polymerase chain reaction (qRT-PCR). A panel with optimal diagnostic value was developed. Additionally, we used bioinformatic analysis to explore the potential biological functions of the crucial miRNAs.ResultsThe diagnostic panel consisted of miR-212-3p, miR-30c-5p, and miR-206, with an area under the curve (AUC) of 0.838, sensitivity of 83.33%, and specificity of 73.81%. Furthermore, ATF3, GJA1, JPH2, MVB12B, RUNX1T1, SLC8A1, SPATA6, and TPM3 may be potential target genes of these three miRNAs.ConclusionWe developed a three-miRNA panel that could serve as a highly efficient and inexpensive biomarker for BC diagnosis and screening. |
| format | Article |
| id | doaj-art-055912a8c31a4ce4aab52fc4729cbab2 |
| institution | Kabale University |
| issn | 2234-943X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj-art-055912a8c31a4ce4aab52fc4729cbab22024-12-24T05:10:27ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2024-12-011410.3389/fonc.2024.14704571470457Panel containing three serum microRNAs: a promising biomarker for early detection of bladder cancerZhenjian Ge0Zhenjian Ge1Zhenjian Ge2Zhenjian Ge3Shengjie Lin4Shengjie Lin5Shengjie Lin6Shengjie Lin7Chong Lu8Chong Lu9Chong Lu10Chong Lu11Yong Xia12Rongkang Li13Rongkang Li14Rongkang Li15Rongkang Li16Xinji Li17Xinji Li18Xinji Li19Xinji Li20Chen Sun21Chen Sun22Chen Sun23Chen Sun24Zhenyu Wen25Zhenyu Wen26Zhenyu Wen27Zhenyu Wen28Wenkang Chen29Wenkang Chen30Wenkang Chen31Wenkang Chen32Yingqi Li33Yingqi Li34Yingqi Li35Yingqi Li36Mingyang Li37Yu Lin38Jing Dong39Lingzhi Tao40Lingzhi Tao41Lingzhi Tao42Ling Ji43Yongqing Lai44Yongqing Lai45Yongqing Lai46Department of Urology, Peking University Shenzhen Hospital, Shenzhen, ChinaInstitute of Urology, Shenzhen Peking University-The Hong Kong University of Science and Technology Medical Center, Shenzhen, ChinaShenzhen Clinical Research Center for Urology and Nephrology, Shenzhen, ChinaShantou University Medical College, Shantou, ChinaDepartment of Urology, Peking University Shenzhen Hospital, Shenzhen, ChinaInstitute of Urology, Shenzhen Peking University-The Hong Kong University of Science and Technology Medical Center, Shenzhen, ChinaShenzhen Clinical Research Center for Urology and Nephrology, Shenzhen, ChinaShantou University Medical College, Shantou, ChinaDepartment of Urology, Peking University Shenzhen Hospital, Shenzhen, ChinaInstitute of Urology, Shenzhen Peking University-The Hong Kong University of Science and Technology Medical Center, Shenzhen, ChinaShenzhen Clinical Research Center for Urology and Nephrology, Shenzhen, ChinaThe Fifth Clinical Medical College of Anhui Medical University, Hefei, ChinaDepartment of Laboratory Medicine, Peking University Shenzhen Hospital, Shenzhen, ChinaDepartment of Urology, Peking University Shenzhen Hospital, Shenzhen, ChinaInstitute of Urology, Shenzhen Peking University-The Hong Kong University of Science and Technology Medical Center, Shenzhen, ChinaShenzhen Clinical Research Center for Urology and Nephrology, Shenzhen, ChinaThe Fifth Clinical Medical College of Anhui Medical University, Hefei, ChinaDepartment of Urology, Peking University Shenzhen Hospital, Shenzhen, ChinaInstitute of Urology, Shenzhen Peking University-The Hong Kong University of Science and Technology Medical Center, Shenzhen, ChinaShenzhen Clinical Research Center for Urology and Nephrology, Shenzhen, ChinaShantou University Medical College, Shantou, ChinaDepartment of Urology, Peking University Shenzhen Hospital, Shenzhen, ChinaInstitute of Urology, Shenzhen Peking University-The Hong Kong University of Science and Technology Medical Center, Shenzhen, ChinaShenzhen Clinical Research Center for Urology and Nephrology, Shenzhen, ChinaThe Fifth Clinical Medical College of Anhui Medical University, Hefei, ChinaDepartment of Urology, Peking University Shenzhen Hospital, Shenzhen, ChinaInstitute of Urology, Shenzhen Peking University-The Hong Kong University of Science and Technology Medical Center, Shenzhen, ChinaShenzhen Clinical Research Center for Urology and Nephrology, Shenzhen, ChinaShantou University Medical College, Shantou, ChinaDepartment of Urology, Peking University Shenzhen Hospital, Shenzhen, ChinaInstitute of Urology, Shenzhen Peking University-The Hong Kong University of Science and Technology Medical Center, Shenzhen, ChinaShenzhen Clinical Research Center for Urology and Nephrology, Shenzhen, ChinaShantou University Medical College, Shantou, ChinaDepartment of Urology, Peking University Shenzhen Hospital, Shenzhen, ChinaInstitute of Urology, Shenzhen Peking University-The Hong Kong University of Science and Technology Medical Center, Shenzhen, ChinaShenzhen Clinical Research Center for Urology and Nephrology, Shenzhen, ChinaShenzhen University Health Science Center, Shenzhen, ChinaDepartment of Laboratory Medicine, Peking University Shenzhen Hospital, Shenzhen, ChinaDepartment of Laboratory Medicine, Peking University Shenzhen Hospital, Shenzhen, ChinaDepartment of Laboratory Medicine, Peking University Shenzhen Hospital, Shenzhen, ChinaDepartment of Urology, Peking University Shenzhen Hospital, Shenzhen, ChinaInstitute of Urology, Shenzhen Peking University-The Hong Kong University of Science and Technology Medical Center, Shenzhen, ChinaShenzhen Clinical Research Center for Urology and Nephrology, Shenzhen, ChinaDepartment of Laboratory Medicine, Peking University Shenzhen Hospital, Shenzhen, ChinaDepartment of Urology, Peking University Shenzhen Hospital, Shenzhen, ChinaInstitute of Urology, Shenzhen Peking University-The Hong Kong University of Science and Technology Medical Center, Shenzhen, ChinaShenzhen Clinical Research Center for Urology and Nephrology, Shenzhen, ChinaBackgroundBladder cancer (BC) is a common tumor worldwide. Screening for BC currently lacks a highly efficient, non-invasive, and inexpensive method. Serum microRNA (miRNA), which is stable and commonly present, has the potential to serve as a novel marker for BC diagnosis.Materials & methodsBased on a study involving 112 BC patients and 112 healthy subjects, we conducted this research in three phases to identify applicable microRNAs (miRNAs) in serum for BC diagnosis using quantitative reverse transcription polymerase chain reaction (qRT-PCR). A panel with optimal diagnostic value was developed. Additionally, we used bioinformatic analysis to explore the potential biological functions of the crucial miRNAs.ResultsThe diagnostic panel consisted of miR-212-3p, miR-30c-5p, and miR-206, with an area under the curve (AUC) of 0.838, sensitivity of 83.33%, and specificity of 73.81%. Furthermore, ATF3, GJA1, JPH2, MVB12B, RUNX1T1, SLC8A1, SPATA6, and TPM3 may be potential target genes of these three miRNAs.ConclusionWe developed a three-miRNA panel that could serve as a highly efficient and inexpensive biomarker for BC diagnosis and screening.https://www.frontiersin.org/articles/10.3389/fonc.2024.1470457/fullmicroRNAbiomarkerbladder cancerMiR-212-3pmiR-30c-5pmiR-206 |
| spellingShingle | Zhenjian Ge Zhenjian Ge Zhenjian Ge Zhenjian Ge Shengjie Lin Shengjie Lin Shengjie Lin Shengjie Lin Chong Lu Chong Lu Chong Lu Chong Lu Yong Xia Rongkang Li Rongkang Li Rongkang Li Rongkang Li Xinji Li Xinji Li Xinji Li Xinji Li Chen Sun Chen Sun Chen Sun Chen Sun Zhenyu Wen Zhenyu Wen Zhenyu Wen Zhenyu Wen Wenkang Chen Wenkang Chen Wenkang Chen Wenkang Chen Yingqi Li Yingqi Li Yingqi Li Yingqi Li Mingyang Li Yu Lin Jing Dong Lingzhi Tao Lingzhi Tao Lingzhi Tao Ling Ji Yongqing Lai Yongqing Lai Yongqing Lai Panel containing three serum microRNAs: a promising biomarker for early detection of bladder cancer Frontiers in Oncology microRNA biomarker bladder cancer MiR-212-3p miR-30c-5p miR-206 |
| title | Panel containing three serum microRNAs: a promising biomarker for early detection of bladder cancer |
| title_full | Panel containing three serum microRNAs: a promising biomarker for early detection of bladder cancer |
| title_fullStr | Panel containing three serum microRNAs: a promising biomarker for early detection of bladder cancer |
| title_full_unstemmed | Panel containing three serum microRNAs: a promising biomarker for early detection of bladder cancer |
| title_short | Panel containing three serum microRNAs: a promising biomarker for early detection of bladder cancer |
| title_sort | panel containing three serum micrornas a promising biomarker for early detection of bladder cancer |
| topic | microRNA biomarker bladder cancer MiR-212-3p miR-30c-5p miR-206 |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2024.1470457/full |
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