Protocol for detecting glycoRNAs using metabolic labeling and northwestern blot

Summary: GlycoRNAs are glycosylated RNAs that can be detected in many cell types and often partly reside on the outer cell surface, with a recently demonstrated role in mediating neutrophil-endothelium interaction. Here, we present a protocol for glycoRNA detection based on metabolic tracing and nor...

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Bibliographic Details
Main Authors: Luoyi Li, Ningning Zhang, Carla Pantoja, Yadong Wang, Jun Lu
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:STAR Protocols
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Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724004866
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