Determination of Riboflavin and Cyanocobalamine Spectrophotometricaly via Area Under Curve Method
A simple, sensitive, accurate and economical spectrophotometric method for determination of Riboflavin (RIBO) and Cyanocobalamin (CCA) in pure and pharmaceutical forms was developed .The method based on the area under curve (AUC) in the region between 400-526 nm for RIBO and 538-562nm for CCA. The...
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Tikrit University
2023-02-01
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| Series: | Tikrit Journal of Pure Science |
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| Online Access: | https://tjpsj.org/index.php/tjps/article/view/899 |
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| author | Mohammed Qasim Al-Samarrai Khlaf F Al-Samarrai |
| author_facet | Mohammed Qasim Al-Samarrai Khlaf F Al-Samarrai |
| author_sort | Mohammed Qasim Al-Samarrai |
| collection | DOAJ |
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A simple, sensitive, accurate and economical spectrophotometric method for determination of Riboflavin (RIBO) and Cyanocobalamin (CCA) in pure and pharmaceutical forms was developed .The method based on the area under curve (AUC) in the region between 400-526 nm for RIBO and 538-562nm for CCA. The method obey Beers law in the concentration range between 10- 90 µg.ml-1 of RIBO and 6- 96 µg.ml-1 of CCA. The Rec% range was between 97.6542-103.5198 % and RSD% 0.5871-0.7542 % , LOD 0.2168 µg.ml-1, LOQ 0.8560 µg.ml-1 of RIBO. The Rec% range was between 99.7835-102.3990 % and RSD% 0.4811-0.8238 % and LOD 0.1381 µg.ml-1, LOQ 0.4606 µg.ml-1 of CCA .
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| format | Article |
| id | doaj-art-0511f49f95ea4f1e9efd0d73d82d538f |
| institution | Kabale University |
| issn | 1813-1662 2415-1726 |
| language | English |
| publishDate | 2023-02-01 |
| publisher | Tikrit University |
| record_format | Article |
| series | Tikrit Journal of Pure Science |
| spelling | doaj-art-0511f49f95ea4f1e9efd0d73d82d538f2025-08-20T03:50:20ZengTikrit UniversityTikrit Journal of Pure Science1813-16622415-17262023-02-01221010.25130/tjps.v22i10.899Determination of Riboflavin and Cyanocobalamine Spectrophotometricaly via Area Under Curve MethodMohammed Qasim Al-SamarraiKhlaf F Al-Samarrai A simple, sensitive, accurate and economical spectrophotometric method for determination of Riboflavin (RIBO) and Cyanocobalamin (CCA) in pure and pharmaceutical forms was developed .The method based on the area under curve (AUC) in the region between 400-526 nm for RIBO and 538-562nm for CCA. The method obey Beers law in the concentration range between 10- 90 µg.ml-1 of RIBO and 6- 96 µg.ml-1 of CCA. The Rec% range was between 97.6542-103.5198 % and RSD% 0.5871-0.7542 % , LOD 0.2168 µg.ml-1, LOQ 0.8560 µg.ml-1 of RIBO. The Rec% range was between 99.7835-102.3990 % and RSD% 0.4811-0.8238 % and LOD 0.1381 µg.ml-1, LOQ 0.4606 µg.ml-1 of CCA . https://tjpsj.org/index.php/tjps/article/view/899area under curveRiboflavinCyanocobalamine |
| spellingShingle | Mohammed Qasim Al-Samarrai Khlaf F Al-Samarrai Determination of Riboflavin and Cyanocobalamine Spectrophotometricaly via Area Under Curve Method Tikrit Journal of Pure Science area under curve Riboflavin Cyanocobalamine |
| title | Determination of Riboflavin and Cyanocobalamine Spectrophotometricaly via Area Under Curve Method |
| title_full | Determination of Riboflavin and Cyanocobalamine Spectrophotometricaly via Area Under Curve Method |
| title_fullStr | Determination of Riboflavin and Cyanocobalamine Spectrophotometricaly via Area Under Curve Method |
| title_full_unstemmed | Determination of Riboflavin and Cyanocobalamine Spectrophotometricaly via Area Under Curve Method |
| title_short | Determination of Riboflavin and Cyanocobalamine Spectrophotometricaly via Area Under Curve Method |
| title_sort | determination of riboflavin and cyanocobalamine spectrophotometricaly via area under curve method |
| topic | area under curve Riboflavin Cyanocobalamine |
| url | https://tjpsj.org/index.php/tjps/article/view/899 |
| work_keys_str_mv | AT mohammedqasimalsamarrai determinationofriboflavinandcyanocobalaminespectrophotometricalyviaareaundercurvemethod AT khlaffalsamarrai determinationofriboflavinandcyanocobalaminespectrophotometricalyviaareaundercurvemethod |