Microfluidic Chip for Quantitatively Assessing Hemorheological Parameters
The biomechanical properties of blood are regarded as promising biomarkers for monitoring early-stage abnormalities and disease progression. To detect any changes in blood, it is necessary to measure as many rheological properties as possible. Herein, a novel method is proposed for measuring multipl...
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| Format: | Article |
| Language: | English |
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MDPI AG
2025-05-01
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| Series: | Micromachines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-666X/16/5/567 |
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| Summary: | The biomechanical properties of blood are regarded as promising biomarkers for monitoring early-stage abnormalities and disease progression. To detect any changes in blood, it is necessary to measure as many rheological properties as possible. Herein, a novel method is proposed for measuring multiple rheological properties of blood using a microfluidic chip. The syringe pump turns off for 5 min to induce RBC (red blood cell) sedimentation in the driving syringe. RBC aggregation is determined by analyzing the time-lapse blood image intensity at stasis: <i>I</i>(<i>t</i>) = <i>I</i><sub>1</sub> exp (−<i>k</i><sub>1</sub><i>t</i>) + <i>I</i><sub>2</sub> exp (−<i>k</i><sub>2</sub><i>t</i>). RBC-rich blood and RBC-depleted blood are sequentially infused into the microfluidic chip. Based on blood pressure estimated with time-lapse blood velocity, blood viscosity is acquired with the Hagen–Poiseuille law. RBC sedimentation is quantified as RBC sedimentation distance (<i>X<sub>esr</sub></i>) and erythrocyte sedimentation rate (ESR). The proposed method provides a consistent viscosity compared with previous methods. Two of the four variables (<i>I</i><sub>1</sub>, <i>I</i><sub>2</sub>) exhibited a strong correlation with the conventional RBC aggregation index (AI). The indices <i>X<sub>esr</sub></i> and ESR showed consistent trends with respect to the blood medium and hematocrit. In conclusion, the proposed method is then regarded as effective for monitoring multiple rheological properties. |
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| ISSN: | 2072-666X |