Unveiling the molecular mechanisms of recurrent miscarriage through endoplasmic reticulum stress related gene expression
Abstract Recurrent miscarriage (RM) is a reproductive disorder affecting couples worldwide. The underlying molecular mechanisms remain elusive, even though emerging evidence has implicated endoplasmic reticulum stress (ERS). We investigated RM- and ERS-related genes to develop a diagnostic model tha...
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Nature Portfolio
2025-01-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-77642-w |
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| author | Xiaodan Yin Wei Yang Mingwei Xin Qian Han Siqi Guan Junqin He |
| author_facet | Xiaodan Yin Wei Yang Mingwei Xin Qian Han Siqi Guan Junqin He |
| author_sort | Xiaodan Yin |
| collection | DOAJ |
| description | Abstract Recurrent miscarriage (RM) is a reproductive disorder affecting couples worldwide. The underlying molecular mechanisms remain elusive, even though emerging evidence has implicated endoplasmic reticulum stress (ERS). We investigated RM- and ERS-related genes to develop a diagnostic model that can enhance predictive ability. We utilized the R package GEO query to extract and process Gene Expression Omnibus data, applying batch correction, normalization, and differential gene expression analysis with limma. ERS-related differentially expressed genes (ERSRGs) were identified through Gene Ontology and Kyoto Encyclopedia of genes and genomes analyses, and their diagnostic potential was assessed. Diagnostic models were developed using logistic regression, support vector machines, and least absolute shrinkage and selection operators, complemented by immune infiltration analysis and regulatory network construction. Integrated analysis revealed 1395 differentially expressed genes (DEGs), including 626 upregulated and 769 downregulated genes. Seventeen ERSRGs were identified. KEAP1 and YIPF5 displayed high diagnostic accuracy (area under the curve [AUC] > 0.9). Gene Ontology and Kyoto Encyclopedia of genes and genomes analyses highlighted the role of ESRDEGs in cellular responses to ERS, protein processing, and apoptosis. Diagnostic models demonstrated robust predictive performance (AUC > 0.9). A molecular interaction was found between RM and the ERS response, and the identified ESRDEGs could serve as potential biomarkers for diagnosis. |
| format | Article |
| id | doaj-art-04e0543a554f4c5f873cd3cd2f0e431a |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-04e0543a554f4c5f873cd3cd2f0e431a2025-01-12T12:14:27ZengNature PortfolioScientific Reports2045-23222025-01-0115111910.1038/s41598-024-77642-wUnveiling the molecular mechanisms of recurrent miscarriage through endoplasmic reticulum stress related gene expressionXiaodan Yin0Wei Yang1Mingwei Xin2Qian Han3Siqi Guan4Junqin He5Department of TCM, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing Maternal and Child Health Care HospitalDepartment of TCM, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing Maternal and Child Health Care HospitalDepartment of TCM, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing Maternal and Child Health Care HospitalDepartment of TCM, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing Maternal and Child Health Care HospitalDepartment of TCM, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing Maternal and Child Health Care HospitalDepartment of TCM, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing Maternal and Child Health Care HospitalAbstract Recurrent miscarriage (RM) is a reproductive disorder affecting couples worldwide. The underlying molecular mechanisms remain elusive, even though emerging evidence has implicated endoplasmic reticulum stress (ERS). We investigated RM- and ERS-related genes to develop a diagnostic model that can enhance predictive ability. We utilized the R package GEO query to extract and process Gene Expression Omnibus data, applying batch correction, normalization, and differential gene expression analysis with limma. ERS-related differentially expressed genes (ERSRGs) were identified through Gene Ontology and Kyoto Encyclopedia of genes and genomes analyses, and their diagnostic potential was assessed. Diagnostic models were developed using logistic regression, support vector machines, and least absolute shrinkage and selection operators, complemented by immune infiltration analysis and regulatory network construction. Integrated analysis revealed 1395 differentially expressed genes (DEGs), including 626 upregulated and 769 downregulated genes. Seventeen ERSRGs were identified. KEAP1 and YIPF5 displayed high diagnostic accuracy (area under the curve [AUC] > 0.9). Gene Ontology and Kyoto Encyclopedia of genes and genomes analyses highlighted the role of ESRDEGs in cellular responses to ERS, protein processing, and apoptosis. Diagnostic models demonstrated robust predictive performance (AUC > 0.9). A molecular interaction was found between RM and the ERS response, and the identified ESRDEGs could serve as potential biomarkers for diagnosis.https://doi.org/10.1038/s41598-024-77642-wRecurrent miscarriageEndoplasmic reticulum stressDiagnostic modelImmune InfiltrationBioinformatics |
| spellingShingle | Xiaodan Yin Wei Yang Mingwei Xin Qian Han Siqi Guan Junqin He Unveiling the molecular mechanisms of recurrent miscarriage through endoplasmic reticulum stress related gene expression Scientific Reports Recurrent miscarriage Endoplasmic reticulum stress Diagnostic model Immune Infiltration Bioinformatics |
| title | Unveiling the molecular mechanisms of recurrent miscarriage through endoplasmic reticulum stress related gene expression |
| title_full | Unveiling the molecular mechanisms of recurrent miscarriage through endoplasmic reticulum stress related gene expression |
| title_fullStr | Unveiling the molecular mechanisms of recurrent miscarriage through endoplasmic reticulum stress related gene expression |
| title_full_unstemmed | Unveiling the molecular mechanisms of recurrent miscarriage through endoplasmic reticulum stress related gene expression |
| title_short | Unveiling the molecular mechanisms of recurrent miscarriage through endoplasmic reticulum stress related gene expression |
| title_sort | unveiling the molecular mechanisms of recurrent miscarriage through endoplasmic reticulum stress related gene expression |
| topic | Recurrent miscarriage Endoplasmic reticulum stress Diagnostic model Immune Infiltration Bioinformatics |
| url | https://doi.org/10.1038/s41598-024-77642-w |
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