Profiling, identification, and quantification of antioxidant components in Gnaphalium affine by HPLC-ECD-MS/MS
Gnaphalium affine is a plant with various active properties and has been approved for use as a traditional medicine and food ingredient. In this study, liquid chromatography with electrochemical detection was utilized to separate the antioxidant compounds of G. affine, in conjunction with liquid chr...
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Taylor & Francis Group
2024-12-01
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| Series: | International Journal of Food Properties |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/10942912.2024.2394533 |
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| author | Qi-Ju Shao Yan Wang Xi-Jin Yang Ke Su Jin-Fen Zheng Li Yang En-Die Zhou Xiao-Yan Yuan Rong-Xiang Chen |
| author_facet | Qi-Ju Shao Yan Wang Xi-Jin Yang Ke Su Jin-Fen Zheng Li Yang En-Die Zhou Xiao-Yan Yuan Rong-Xiang Chen |
| author_sort | Qi-Ju Shao |
| collection | DOAJ |
| description | Gnaphalium affine is a plant with various active properties and has been approved for use as a traditional medicine and food ingredient. In this study, liquid chromatography with electrochemical detection was utilized to separate the antioxidant compounds of G. affine, in conjunction with liquid chromatography-triple quadrupole mass spectrometry for identification. The in vitro antioxidant activity of G. affine was evaluated using DPPH and ABTS radical scavenging capacity assays, as well as the ferric reducing antioxidant power assay. The results showed that a total of 25 antioxidant compounds were screened and identified, among which 14 compounds were quantitatively analyzed. G. affine was found to be rich in phenolic substances, with the total phenolic content ranging from 14.62 to 59.30 mg GAE/g DW, which exhibited high antioxidant potential. The antioxidant activities showed a strong positive correlation with the total phenolic content (r > 0.9). The predominant phenolic compounds in G. affine were 5-O-caffeoylquinic acid, 3-O-caffeoylquinic acid, ferulic acid, luteolin-7-O-glucoside, 3,5-O-dicaffeoylquinic acid, 1,5-O-dicaffeoylquinic acid, and luteolin, with 3,5-O-dicaffeoylquinic acid and 1,5-O-dicaffeoylquinic acid exhibiting the highest levels. Statistical analysis indicated that monosubstituted caffeoylquinic acids, disubstituted caffeoylquinic acids, and luteolin derivatives strongly correlated with the antioxidant potential. This study identified and quantified the major chemical constituents responsible for the antioxidant properties of G. affine, demonstrating its potential as a natural source of antioxidants for the use in foods, pharmaceuticals, and health products. |
| format | Article |
| id | doaj-art-04cf9121e7b843cd92aef1c95626d27d |
| institution | Kabale University |
| issn | 1094-2912 1532-2386 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | International Journal of Food Properties |
| spelling | doaj-art-04cf9121e7b843cd92aef1c95626d27d2024-11-18T20:58:53ZengTaylor & Francis GroupInternational Journal of Food Properties1094-29121532-23862024-12-012711200121410.1080/10942912.2024.2394533Profiling, identification, and quantification of antioxidant components in Gnaphalium affine by HPLC-ECD-MS/MSQi-Ju Shao0Yan Wang1Xi-Jin Yang2Ke Su3Jin-Fen Zheng4Li Yang5En-Die Zhou6Xiao-Yan Yuan7Rong-Xiang Chen8Department of Analytical Chemistry, School of Pharmacy, Zunyi Medical University, Zunyi, ChinaDepartment of Analytical Chemistry, School of Pharmacy, Zunyi Medical University, Zunyi, ChinaDepartment of Analytical Chemistry, School of Pharmacy, Zunyi Medical University, Zunyi, ChinaDepartment of Analytical Chemistry, School of Pharmacy, Zunyi Medical University, Zunyi, ChinaDepartment of Analytical Chemistry, School of Pharmacy, Zunyi Medical University, Zunyi, ChinaDepartment of Analytical Chemistry, School of Pharmacy, Zunyi Medical University, Zunyi, ChinaDepartment of Analytical Chemistry, School of Pharmacy, Zunyi Medical University, Zunyi, ChinaDepartment of Analytical Chemistry, School of Pharmacy, Zunyi Medical University, Zunyi, ChinaAnalysis and Testing Center, School of Basic Medical Sciences, Zunyi Medical University, Zunyi, ChinaGnaphalium affine is a plant with various active properties and has been approved for use as a traditional medicine and food ingredient. In this study, liquid chromatography with electrochemical detection was utilized to separate the antioxidant compounds of G. affine, in conjunction with liquid chromatography-triple quadrupole mass spectrometry for identification. The in vitro antioxidant activity of G. affine was evaluated using DPPH and ABTS radical scavenging capacity assays, as well as the ferric reducing antioxidant power assay. The results showed that a total of 25 antioxidant compounds were screened and identified, among which 14 compounds were quantitatively analyzed. G. affine was found to be rich in phenolic substances, with the total phenolic content ranging from 14.62 to 59.30 mg GAE/g DW, which exhibited high antioxidant potential. The antioxidant activities showed a strong positive correlation with the total phenolic content (r > 0.9). The predominant phenolic compounds in G. affine were 5-O-caffeoylquinic acid, 3-O-caffeoylquinic acid, ferulic acid, luteolin-7-O-glucoside, 3,5-O-dicaffeoylquinic acid, 1,5-O-dicaffeoylquinic acid, and luteolin, with 3,5-O-dicaffeoylquinic acid and 1,5-O-dicaffeoylquinic acid exhibiting the highest levels. Statistical analysis indicated that monosubstituted caffeoylquinic acids, disubstituted caffeoylquinic acids, and luteolin derivatives strongly correlated with the antioxidant potential. This study identified and quantified the major chemical constituents responsible for the antioxidant properties of G. affine, demonstrating its potential as a natural source of antioxidants for the use in foods, pharmaceuticals, and health products.https://www.tandfonline.com/doi/10.1080/10942912.2024.2394533Gnaphalium affineelectrochemical detectionLC-MS/MSantioxidant activitygray relational analysis |
| spellingShingle | Qi-Ju Shao Yan Wang Xi-Jin Yang Ke Su Jin-Fen Zheng Li Yang En-Die Zhou Xiao-Yan Yuan Rong-Xiang Chen Profiling, identification, and quantification of antioxidant components in Gnaphalium affine by HPLC-ECD-MS/MS International Journal of Food Properties Gnaphalium affine electrochemical detection LC-MS/MS antioxidant activity gray relational analysis |
| title | Profiling, identification, and quantification of antioxidant components in Gnaphalium affine by HPLC-ECD-MS/MS |
| title_full | Profiling, identification, and quantification of antioxidant components in Gnaphalium affine by HPLC-ECD-MS/MS |
| title_fullStr | Profiling, identification, and quantification of antioxidant components in Gnaphalium affine by HPLC-ECD-MS/MS |
| title_full_unstemmed | Profiling, identification, and quantification of antioxidant components in Gnaphalium affine by HPLC-ECD-MS/MS |
| title_short | Profiling, identification, and quantification of antioxidant components in Gnaphalium affine by HPLC-ECD-MS/MS |
| title_sort | profiling identification and quantification of antioxidant components in gnaphalium affine by hplc ecd ms ms |
| topic | Gnaphalium affine electrochemical detection LC-MS/MS antioxidant activity gray relational analysis |
| url | https://www.tandfonline.com/doi/10.1080/10942912.2024.2394533 |
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