Ferulic Acid and Clinoptilolite Affect In Vitro Rumen Fermentation Characteristics and Bacterial Abundance
This study evaluated the effects of clinoptilolite (CTL) and ferulic acid (FA) supplementation on in vitro ruminal fermentation characteristics, gas production, and bacterial abundance. Treatments were arranged in a 2 × 2 factorial design (FA: 0 or 300 ppm; CTL: 0 or 1%) with repeated measures over...
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2024-10-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/10/11/549 |
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| author | Ana Tánori-Lozano M. Ángeles López-Baca Adriana Muhlia-Almazán Maricela Montalvo-Corral Araceli Pinelli-Saavedra Thalia Y. Islava-Lagarda José Luis Dávila-Ramírez Martín Valenzuela-Melendres Humberto González-Rios |
| author_facet | Ana Tánori-Lozano M. Ángeles López-Baca Adriana Muhlia-Almazán Maricela Montalvo-Corral Araceli Pinelli-Saavedra Thalia Y. Islava-Lagarda José Luis Dávila-Ramírez Martín Valenzuela-Melendres Humberto González-Rios |
| author_sort | Ana Tánori-Lozano |
| collection | DOAJ |
| description | This study evaluated the effects of clinoptilolite (CTL) and ferulic acid (FA) supplementation on in vitro ruminal fermentation characteristics, gas production, and bacterial abundance. Treatments were arranged in a 2 × 2 factorial design (FA: 0 or 300 ppm; CTL: 0 or 1%) with repeated measures over time (2, 4, 8, 12, 24, 36, 48, and 72 h). Throughout the incubation period, the CTL and FAZ treatments recorded the highest pH values (<i>p</i> ≤ 0.05), maintaining levels closest to neutrality after 72 h. After 48 and 72 h, FA and CTL decreased (<i>p</i> ≤ 0.05) the ammonia concentrations while increasing (<i>p</i> ≤ 0.05) acetate and propionate. The methane, butyrate, and iso-VFA concentrations were unaffected (<i>p</i> > 0.05) by any treatment. FA increased the total gas production throughout the experimental period (<i>p</i> ≤ 0.05). Additionally, FA and CTL significantly reduced the relative abundance of <i>Ruminococcus albus</i> and <i>Streptococcus bovis</i> (<i>p</i> ≤ 0.05), while no significant effects were observed for <i>Selenomonas ruminantium</i> (<i>p</i> > 0.05). These findings suggest that both additives can positively modify the rumen fermentation characteristics and microbial composition, which could significantly contribute to animal nutrition by providing a promising strategy for enhancing rumen fermentation. |
| format | Article |
| id | doaj-art-04b624f384ff401992b2e8f3162d6e9e |
| institution | Kabale University |
| issn | 2311-5637 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj-art-04b624f384ff401992b2e8f3162d6e9e2024-11-26T18:03:26ZengMDPI AGFermentation2311-56372024-10-01101154910.3390/fermentation10110549Ferulic Acid and Clinoptilolite Affect In Vitro Rumen Fermentation Characteristics and Bacterial AbundanceAna Tánori-Lozano0M. Ángeles López-Baca1Adriana Muhlia-Almazán2Maricela Montalvo-Corral3Araceli Pinelli-Saavedra4Thalia Y. Islava-Lagarda5José Luis Dávila-Ramírez6Martín Valenzuela-Melendres7Humberto González-Rios8Centro de Investigación en Alimentación y Desarrollo, A.C (CIAD, A.C.), Carretera Gustavo Enrique Astiazarán # 46, Colonia La Victoria, Hermosillo Sonora 83304, MexicoInstituto de Ciencias Agrícolas, Universidad Autónoma de Baja California, Valle de Mexicali, Baja California 21705, MexicoCentro de Investigación en Alimentación y Desarrollo, A.C (CIAD, A.C.), Carretera Gustavo Enrique Astiazarán # 46, Colonia La Victoria, Hermosillo Sonora 83304, MexicoCentro de Investigación en Alimentación y Desarrollo, A.C (CIAD, A.C.), Carretera Gustavo Enrique Astiazarán # 46, Colonia La Victoria, Hermosillo Sonora 83304, MexicoCentro de Investigación en Alimentación y Desarrollo, A.C (CIAD, A.C.), Carretera Gustavo Enrique Astiazarán # 46, Colonia La Victoria, Hermosillo Sonora 83304, MexicoCentro de Investigación en Alimentación y Desarrollo, A.C (CIAD, A.C.), Carretera Gustavo Enrique Astiazarán # 46, Colonia La Victoria, Hermosillo Sonora 83304, MexicoCentro de Investigación en Alimentación y Desarrollo, A.C (CIAD, A.C.), Carretera Gustavo Enrique Astiazarán # 46, Colonia La Victoria, Hermosillo Sonora 83304, MexicoCentro de Investigación en Alimentación y Desarrollo, A.C (CIAD, A.C.), Carretera Gustavo Enrique Astiazarán # 46, Colonia La Victoria, Hermosillo Sonora 83304, MexicoCentro de Investigación en Alimentación y Desarrollo, A.C (CIAD, A.C.), Carretera Gustavo Enrique Astiazarán # 46, Colonia La Victoria, Hermosillo Sonora 83304, MexicoThis study evaluated the effects of clinoptilolite (CTL) and ferulic acid (FA) supplementation on in vitro ruminal fermentation characteristics, gas production, and bacterial abundance. Treatments were arranged in a 2 × 2 factorial design (FA: 0 or 300 ppm; CTL: 0 or 1%) with repeated measures over time (2, 4, 8, 12, 24, 36, 48, and 72 h). Throughout the incubation period, the CTL and FAZ treatments recorded the highest pH values (<i>p</i> ≤ 0.05), maintaining levels closest to neutrality after 72 h. After 48 and 72 h, FA and CTL decreased (<i>p</i> ≤ 0.05) the ammonia concentrations while increasing (<i>p</i> ≤ 0.05) acetate and propionate. The methane, butyrate, and iso-VFA concentrations were unaffected (<i>p</i> > 0.05) by any treatment. FA increased the total gas production throughout the experimental period (<i>p</i> ≤ 0.05). Additionally, FA and CTL significantly reduced the relative abundance of <i>Ruminococcus albus</i> and <i>Streptococcus bovis</i> (<i>p</i> ≤ 0.05), while no significant effects were observed for <i>Selenomonas ruminantium</i> (<i>p</i> > 0.05). These findings suggest that both additives can positively modify the rumen fermentation characteristics and microbial composition, which could significantly contribute to animal nutrition by providing a promising strategy for enhancing rumen fermentation.https://www.mdpi.com/2311-5637/10/11/549zeolitephytochemicalrumen fermentationrumen microbiome |
| spellingShingle | Ana Tánori-Lozano M. Ángeles López-Baca Adriana Muhlia-Almazán Maricela Montalvo-Corral Araceli Pinelli-Saavedra Thalia Y. Islava-Lagarda José Luis Dávila-Ramírez Martín Valenzuela-Melendres Humberto González-Rios Ferulic Acid and Clinoptilolite Affect In Vitro Rumen Fermentation Characteristics and Bacterial Abundance Fermentation zeolite phytochemical rumen fermentation rumen microbiome |
| title | Ferulic Acid and Clinoptilolite Affect In Vitro Rumen Fermentation Characteristics and Bacterial Abundance |
| title_full | Ferulic Acid and Clinoptilolite Affect In Vitro Rumen Fermentation Characteristics and Bacterial Abundance |
| title_fullStr | Ferulic Acid and Clinoptilolite Affect In Vitro Rumen Fermentation Characteristics and Bacterial Abundance |
| title_full_unstemmed | Ferulic Acid and Clinoptilolite Affect In Vitro Rumen Fermentation Characteristics and Bacterial Abundance |
| title_short | Ferulic Acid and Clinoptilolite Affect In Vitro Rumen Fermentation Characteristics and Bacterial Abundance |
| title_sort | ferulic acid and clinoptilolite affect in vitro rumen fermentation characteristics and bacterial abundance |
| topic | zeolite phytochemical rumen fermentation rumen microbiome |
| url | https://www.mdpi.com/2311-5637/10/11/549 |
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