Protocol for cell proliferation and cell death analysis of primary muscle stem cell culture using flow cytometry

Protocol for cell proliferation and cell death analysis of primary muscle stem cell culture using flow cytometry

Summary: Skeletal muscle is critically dependent on the function of muscle stem cells (MuSCs) for effective muscle repair following injury. Here, we detail a protocol for the isolation of primary muscle cells and subsequent analysis of proliferation capacity in vitro using EdU (5-ethynyl-2′-deoxyuri...

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Bibliographic Details
Main Authors: Pauline Garcia, Orane Mercier, Jade Ravent, Fabien Le Grand
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:STAR Protocols
Subjects:
cell biology
cell culture
cell isolation
flow cytometry
microscopy
stem cells
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724005768
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