Eugenol inhibits NEAT1 as a ceRNA in pre-cancerous breast lesions
Objective: Eugenol (EU) from cloves is highly effective against different tumors. The long noncoding ribonucleic acids (lncRNAs), which play a role of competing endogenous RNAs (ceRNAs), suppress microRNAs (miRNAs) involved in post-transcriptional regulatory networks. The present work focused on ana...
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| Language: | English |
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Elsevier
2025-01-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024173847 |
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| author | Yusheng Liu Guijuan Zhang Yi Ma Min Ma Xuefeng Jiang |
| author_facet | Yusheng Liu Guijuan Zhang Yi Ma Min Ma Xuefeng Jiang |
| author_sort | Yusheng Liu |
| collection | DOAJ |
| description | Objective: Eugenol (EU) from cloves is highly effective against different tumors. The long noncoding ribonucleic acids (lncRNAs), which play a role of competing endogenous RNAs (ceRNAs), suppress microRNAs (miRNAs) involved in post-transcriptional regulatory networks. The present work focused on analyzing how EU affected pre-cancerous breast lesions (PBL). Methods: Initially, the gene expression profiles of patients (n = 880) in the National Center for Biotechnology Information (NCBI) database were analyzed. Further, we established a lncRNA-miRNA-mRNA ceRNA network through bioinformatics analysis and investigated mechanistic roles of lncRNAs as ceRNAs and the anti-tumor effect of EU using MCF-10AT cells in vitro as well as PBL model rats in vivo. Besides, Nuclear Paraspeckle Assembly Transcript 1 (NEAT1), miR-383-5p, miR-9-5p, matrix metalloproteinase-9 (MMP-9), and vascular endothelial growth factor-A (VEGF-A) expression was examined through quantitative reverse transcription polymerase chain reaction (RT-qPCR), Western blotting, and immunohistochemical staining analyses. Results: There were altogether 1162 mRNAs, 81 miRNAs, and 26 lncRNAs recognized as trend genes in breast cancer (BC) and pre-cancerous BC (pBC), constructing the ceRNA network using 3 lncRNAs, 3 miRNAs, and 38 mRNAs. It was observed that NEAT1, miR-383-5p, miR-9-5p, VEGF-A, and MMP-9 were downregulated in breast tumor cells in accordance with bioinformatics analysis. EU suppressed MCF-10AT cell growth, decreasing the NEAT1, VEGF-A, and MMP-9 levels and increasing miR-383-5p and miR-9-5p expressions in vitro and in vivo. Conclusion: In summary, the EU reduced the VEGF-A and MMP-9 expressions via NEAT1-mediated miR-383-5p and miR-9-5p against PBL, indicating that the EU may be a promising external drug to act against PBL. |
| format | Article |
| id | doaj-art-0471f40944f54a149350ca2746795fd2 |
| institution | Kabale University |
| issn | 2405-8440 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj-art-0471f40944f54a149350ca2746795fd22025-01-17T04:51:01ZengElsevierHeliyon2405-84402025-01-01111e41353Eugenol inhibits NEAT1 as a ceRNA in pre-cancerous breast lesionsYusheng Liu0Guijuan Zhang1Yi Ma2Min Ma3Xuefeng Jiang4Department of Traditional Chinese Medicine, Yangjiang People's Hospital, Yangjiang, Guangdong, 529525, China; College of Traditional Chinese Medicine, Jinan University, Guangzhou, Guangdong, 510515, ChinaCollege of Traditional Chinese Medicine, Jinan University, Guangzhou, Guangdong, 510515, ChinaInstitute of Biomedicine and Department of Cellular Biology, Jinan University, Guangzhou, Guangdong, 510515, ChinaCollege of Traditional Chinese Medicine, Jinan University, Guangzhou, Guangdong, 510515, China; Corresponding author. No.42, Dongshan Road, Jiangcheng District, Yangjiang, Guangdong, 529525, China.Department of Traditional Chinese Medicine, Yangjiang People's Hospital, Yangjiang, Guangdong, 529525, China; College of Traditional Chinese Medicine, Jinan University, Guangzhou, Guangdong, 510515, China; Corresponding author. No.42, Dongshan Road, Jiangcheng District, Yangjiang, Guangdong, 529525, China.Objective: Eugenol (EU) from cloves is highly effective against different tumors. The long noncoding ribonucleic acids (lncRNAs), which play a role of competing endogenous RNAs (ceRNAs), suppress microRNAs (miRNAs) involved in post-transcriptional regulatory networks. The present work focused on analyzing how EU affected pre-cancerous breast lesions (PBL). Methods: Initially, the gene expression profiles of patients (n = 880) in the National Center for Biotechnology Information (NCBI) database were analyzed. Further, we established a lncRNA-miRNA-mRNA ceRNA network through bioinformatics analysis and investigated mechanistic roles of lncRNAs as ceRNAs and the anti-tumor effect of EU using MCF-10AT cells in vitro as well as PBL model rats in vivo. Besides, Nuclear Paraspeckle Assembly Transcript 1 (NEAT1), miR-383-5p, miR-9-5p, matrix metalloproteinase-9 (MMP-9), and vascular endothelial growth factor-A (VEGF-A) expression was examined through quantitative reverse transcription polymerase chain reaction (RT-qPCR), Western blotting, and immunohistochemical staining analyses. Results: There were altogether 1162 mRNAs, 81 miRNAs, and 26 lncRNAs recognized as trend genes in breast cancer (BC) and pre-cancerous BC (pBC), constructing the ceRNA network using 3 lncRNAs, 3 miRNAs, and 38 mRNAs. It was observed that NEAT1, miR-383-5p, miR-9-5p, VEGF-A, and MMP-9 were downregulated in breast tumor cells in accordance with bioinformatics analysis. EU suppressed MCF-10AT cell growth, decreasing the NEAT1, VEGF-A, and MMP-9 levels and increasing miR-383-5p and miR-9-5p expressions in vitro and in vivo. Conclusion: In summary, the EU reduced the VEGF-A and MMP-9 expressions via NEAT1-mediated miR-383-5p and miR-9-5p against PBL, indicating that the EU may be a promising external drug to act against PBL.http://www.sciencedirect.com/science/article/pii/S2405844024173847EugenolPre-cancerous breast lesionNEAT1ceRNAmiR-383-5pmiR-9-5p |
| spellingShingle | Yusheng Liu Guijuan Zhang Yi Ma Min Ma Xuefeng Jiang Eugenol inhibits NEAT1 as a ceRNA in pre-cancerous breast lesions Heliyon Eugenol Pre-cancerous breast lesion NEAT1 ceRNA miR-383-5p miR-9-5p |
| title | Eugenol inhibits NEAT1 as a ceRNA in pre-cancerous breast lesions |
| title_full | Eugenol inhibits NEAT1 as a ceRNA in pre-cancerous breast lesions |
| title_fullStr | Eugenol inhibits NEAT1 as a ceRNA in pre-cancerous breast lesions |
| title_full_unstemmed | Eugenol inhibits NEAT1 as a ceRNA in pre-cancerous breast lesions |
| title_short | Eugenol inhibits NEAT1 as a ceRNA in pre-cancerous breast lesions |
| title_sort | eugenol inhibits neat1 as a cerna in pre cancerous breast lesions |
| topic | Eugenol Pre-cancerous breast lesion NEAT1 ceRNA miR-383-5p miR-9-5p |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024173847 |
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