Fabrication and characterization of 3D-printed composite scaffolds of coral-derived hydroxyapatite nanoparticles/polycaprolactone/gelatin carrying doxorubicin for bone tissue engineering
Abstract In this study, nanocomposite scaffolds of hydroxyapatite (HA)/polycaprolactone (PCL)/gelatin (Gel) with varying amounts of HA (42–52 wt. %), PCL (42–52 wt. %), and Gel (6 wt. %) were 3D printed. Subsequently, a scaffold with optimal mechanical properties was utilized as a carrier for doxoru...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Springer
2024-01-01
|
| Series: | Journal of Materials Science: Materials in Medicine |
| Online Access: | https://doi.org/10.1007/s10856-024-06779-x |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846112895365021696 |
|---|---|
| author | Fatima Kadi Ghasem Dini S. Ali Poursamar Fatemeh Ejeian |
| author_facet | Fatima Kadi Ghasem Dini S. Ali Poursamar Fatemeh Ejeian |
| author_sort | Fatima Kadi |
| collection | DOAJ |
| description | Abstract In this study, nanocomposite scaffolds of hydroxyapatite (HA)/polycaprolactone (PCL)/gelatin (Gel) with varying amounts of HA (42–52 wt. %), PCL (42–52 wt. %), and Gel (6 wt. %) were 3D printed. Subsequently, a scaffold with optimal mechanical properties was utilized as a carrier for doxorubicin (DOX) in the treatment of bone cancer. For this purpose, HA nanoparticles were first synthesized by the hydrothermal conversion of Acropora coral and characterized by using different techniques. Also, a compression test was performed to investigate the mechanical properties of the fabricated scaffolds. The mineralization of the optimal scaffold was determined by immersing it in simulated body fluid (SBF) solution for 28 days, and the biocompatibility was investigated by seeding MG-63 osteoblast-like cells on it after 1–7 days. The obtained results showed that the average size of the synthesized HA particles was about 80 nm. The compressive modulus and strength of the scaffold with 47 wt. % HA was reported to be 0.29 GPa and 9.9 MPa, respectively, which was in the range of trabecular bones. In addition, the scaffold surface was entirely coated with an apatite layer after 28 days of soaking in SBF. Also, the efficiency and loading percentage of DOX were obtained as 30.8 and 1.6%, respectively. The drug release behavior was stable for 14 days. Cytotoxicity and adhesion evaluations showed that the fabricated scaffold had no negative effects on the viability of MG-63 cells and led to their proliferation during the investigated period. From these results, it can be concluded that the HA/PCL/Gel scaffold prepared in this study, in addition to its drug release capability, has good bioactivity, mechanical properties, and biocompatibility, and can be considered a suitable option for bone tumor treatment. Graphical Abstract |
| format | Article |
| id | doaj-art-044d77da3f534bac9c99c0d3ddd3e37e |
| institution | Kabale University |
| issn | 1573-4838 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Springer |
| record_format | Article |
| series | Journal of Materials Science: Materials in Medicine |
| spelling | doaj-art-044d77da3f534bac9c99c0d3ddd3e37e2024-12-22T12:11:13ZengSpringerJournal of Materials Science: Materials in Medicine1573-48382024-01-0135111510.1007/s10856-024-06779-xFabrication and characterization of 3D-printed composite scaffolds of coral-derived hydroxyapatite nanoparticles/polycaprolactone/gelatin carrying doxorubicin for bone tissue engineeringFatima Kadi0Ghasem Dini1S. Ali Poursamar2Fatemeh Ejeian3Department of Nanotechnology, Faculty of Chemistry, University of IsfahanDepartment of Nanotechnology, Faculty of Chemistry, University of IsfahanDepartment of Biomaterials, Nanotechnology, and Tissue Engineering, School of Advanced Technologies in Medicine, Isfahan University of Medical SciencesDepartment of Animal Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECRAbstract In this study, nanocomposite scaffolds of hydroxyapatite (HA)/polycaprolactone (PCL)/gelatin (Gel) with varying amounts of HA (42–52 wt. %), PCL (42–52 wt. %), and Gel (6 wt. %) were 3D printed. Subsequently, a scaffold with optimal mechanical properties was utilized as a carrier for doxorubicin (DOX) in the treatment of bone cancer. For this purpose, HA nanoparticles were first synthesized by the hydrothermal conversion of Acropora coral and characterized by using different techniques. Also, a compression test was performed to investigate the mechanical properties of the fabricated scaffolds. The mineralization of the optimal scaffold was determined by immersing it in simulated body fluid (SBF) solution for 28 days, and the biocompatibility was investigated by seeding MG-63 osteoblast-like cells on it after 1–7 days. The obtained results showed that the average size of the synthesized HA particles was about 80 nm. The compressive modulus and strength of the scaffold with 47 wt. % HA was reported to be 0.29 GPa and 9.9 MPa, respectively, which was in the range of trabecular bones. In addition, the scaffold surface was entirely coated with an apatite layer after 28 days of soaking in SBF. Also, the efficiency and loading percentage of DOX were obtained as 30.8 and 1.6%, respectively. The drug release behavior was stable for 14 days. Cytotoxicity and adhesion evaluations showed that the fabricated scaffold had no negative effects on the viability of MG-63 cells and led to their proliferation during the investigated period. From these results, it can be concluded that the HA/PCL/Gel scaffold prepared in this study, in addition to its drug release capability, has good bioactivity, mechanical properties, and biocompatibility, and can be considered a suitable option for bone tumor treatment. Graphical Abstracthttps://doi.org/10.1007/s10856-024-06779-x |
| spellingShingle | Fatima Kadi Ghasem Dini S. Ali Poursamar Fatemeh Ejeian Fabrication and characterization of 3D-printed composite scaffolds of coral-derived hydroxyapatite nanoparticles/polycaprolactone/gelatin carrying doxorubicin for bone tissue engineering Journal of Materials Science: Materials in Medicine |
| title | Fabrication and characterization of 3D-printed composite scaffolds of coral-derived hydroxyapatite nanoparticles/polycaprolactone/gelatin carrying doxorubicin for bone tissue engineering |
| title_full | Fabrication and characterization of 3D-printed composite scaffolds of coral-derived hydroxyapatite nanoparticles/polycaprolactone/gelatin carrying doxorubicin for bone tissue engineering |
| title_fullStr | Fabrication and characterization of 3D-printed composite scaffolds of coral-derived hydroxyapatite nanoparticles/polycaprolactone/gelatin carrying doxorubicin for bone tissue engineering |
| title_full_unstemmed | Fabrication and characterization of 3D-printed composite scaffolds of coral-derived hydroxyapatite nanoparticles/polycaprolactone/gelatin carrying doxorubicin for bone tissue engineering |
| title_short | Fabrication and characterization of 3D-printed composite scaffolds of coral-derived hydroxyapatite nanoparticles/polycaprolactone/gelatin carrying doxorubicin for bone tissue engineering |
| title_sort | fabrication and characterization of 3d printed composite scaffolds of coral derived hydroxyapatite nanoparticles polycaprolactone gelatin carrying doxorubicin for bone tissue engineering |
| url | https://doi.org/10.1007/s10856-024-06779-x |
| work_keys_str_mv | AT fatimakadi fabricationandcharacterizationof3dprintedcompositescaffoldsofcoralderivedhydroxyapatitenanoparticlespolycaprolactonegelatincarryingdoxorubicinforbonetissueengineering AT ghasemdini fabricationandcharacterizationof3dprintedcompositescaffoldsofcoralderivedhydroxyapatitenanoparticlespolycaprolactonegelatincarryingdoxorubicinforbonetissueengineering AT salipoursamar fabricationandcharacterizationof3dprintedcompositescaffoldsofcoralderivedhydroxyapatitenanoparticlespolycaprolactonegelatincarryingdoxorubicinforbonetissueengineering AT fatemehejeian fabricationandcharacterizationof3dprintedcompositescaffoldsofcoralderivedhydroxyapatitenanoparticlespolycaprolactonegelatincarryingdoxorubicinforbonetissueengineering |