Fabrication and in vitro evaluation of pH/thermo dual responsive hydrogels as controlled ibuprofen sodium in situ depot

Ibuprofen sodium (IBP) is a commonly used NSAID for multiple pain conditions. However, despite its extensive use, it is associated with multiple GIT adverse effects after oral administration. In the present study, we have fabricated thermoresponsive gel depot using Poly (N-vinylcaprolactam) and sodi...

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Main Authors: Samiullah Khan, Abdur Rehman, Syed Faisal Badshah, Gamal A. Shazly, Amira Metouekel, Fakhreldeen Dabiellil
Format: Article
Language:English
Published: Taylor & Francis Group 2025-12-01
Series:Designed Monomers and Polymers
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Online Access:http://dx.doi.org/10.1080/15685551.2024.2442118
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author Samiullah Khan
Abdur Rehman
Syed Faisal Badshah
Gamal A. Shazly
Amira Metouekel
Fakhreldeen Dabiellil
author_facet Samiullah Khan
Abdur Rehman
Syed Faisal Badshah
Gamal A. Shazly
Amira Metouekel
Fakhreldeen Dabiellil
author_sort Samiullah Khan
collection DOAJ
description Ibuprofen sodium (IBP) is a commonly used NSAID for multiple pain conditions. However, despite its extensive use, it is associated with multiple GIT adverse effects after oral administration. In the present study, we have fabricated thermoresponsive gel depot using Poly (N-vinylcaprolactam) and sodium alginate as polymers. The designed formulations are intended to be used as IBP depot after being administered subcutaneously. The sol-gel phase transition temperature and gelation time of gel samples were optimized by tube inversion, rheological exploration and optical transmittances. Temperature sweep experiments confirmed that optimized gel samples have sol-gel transition between 32°C and 37°C. Swelling and in vitro drug release displayed that optimized gels have maximum swelling and IBP release at pH 7.4 and at 35°C confirming their pH/thermo sensitivity. The degradation profile of hydrogels displayed controlled degradation for 6 days that with increasing contents. MTT assay showed L929 cells displayed more than 90% cell viability against blank and IBP-loaded PNVCL/NaAlg hydrogels at optimized concentrations. Fourier transform infrared spectroscopy confirmed the polymer blend hydrogels structure formation. Thermogravimetric analysis confirmed the presence of thermoresponsive moieties and thermal stability of polymer blend hydrogel sample. While scanning electron microscopy showed that hydrogel has channels in structure that might facilitate the diffusion of solvent. Results concluded that PNVCL/NaAlg hydrogels can be utilized as IBP sustained depot following subcutaneous application invivo and GIT adverse effects could be avoided associated with its oral administration.
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spelling doaj-art-0383c081810f4dcc9b0b6bfb5d42fdd62025-01-02T11:44:28ZengTaylor & Francis GroupDesigned Monomers and Polymers1385-772X1568-55512025-12-0128111510.1080/15685551.2024.24421182442118Fabrication and in vitro evaluation of pH/thermo dual responsive hydrogels as controlled ibuprofen sodium in situ depotSamiullah Khan0Abdur Rehman1Syed Faisal Badshah2Gamal A. Shazly3Amira Metouekel4Fakhreldeen Dabiellil5Margalla Institute of Health SciencesSoochow UniversityUniversity of Poonch RawalakotKing Saud UniversityUniversity of Technology of CompiègneUniversity of Bahr el GhazalIbuprofen sodium (IBP) is a commonly used NSAID for multiple pain conditions. However, despite its extensive use, it is associated with multiple GIT adverse effects after oral administration. In the present study, we have fabricated thermoresponsive gel depot using Poly (N-vinylcaprolactam) and sodium alginate as polymers. The designed formulations are intended to be used as IBP depot after being administered subcutaneously. The sol-gel phase transition temperature and gelation time of gel samples were optimized by tube inversion, rheological exploration and optical transmittances. Temperature sweep experiments confirmed that optimized gel samples have sol-gel transition between 32°C and 37°C. Swelling and in vitro drug release displayed that optimized gels have maximum swelling and IBP release at pH 7.4 and at 35°C confirming their pH/thermo sensitivity. The degradation profile of hydrogels displayed controlled degradation for 6 days that with increasing contents. MTT assay showed L929 cells displayed more than 90% cell viability against blank and IBP-loaded PNVCL/NaAlg hydrogels at optimized concentrations. Fourier transform infrared spectroscopy confirmed the polymer blend hydrogels structure formation. Thermogravimetric analysis confirmed the presence of thermoresponsive moieties and thermal stability of polymer blend hydrogel sample. While scanning electron microscopy showed that hydrogel has channels in structure that might facilitate the diffusion of solvent. Results concluded that PNVCL/NaAlg hydrogels can be utilized as IBP sustained depot following subcutaneous application invivo and GIT adverse effects could be avoided associated with its oral administration.http://dx.doi.org/10.1080/15685551.2024.2442118thermogelspoly(n-vinylcaprolactam)sodium alginatein situ depotrheologymtt assay
spellingShingle Samiullah Khan
Abdur Rehman
Syed Faisal Badshah
Gamal A. Shazly
Amira Metouekel
Fakhreldeen Dabiellil
Fabrication and in vitro evaluation of pH/thermo dual responsive hydrogels as controlled ibuprofen sodium in situ depot
Designed Monomers and Polymers
thermogels
poly(n-vinylcaprolactam)
sodium alginate
in situ depot
rheology
mtt assay
title Fabrication and in vitro evaluation of pH/thermo dual responsive hydrogels as controlled ibuprofen sodium in situ depot
title_full Fabrication and in vitro evaluation of pH/thermo dual responsive hydrogels as controlled ibuprofen sodium in situ depot
title_fullStr Fabrication and in vitro evaluation of pH/thermo dual responsive hydrogels as controlled ibuprofen sodium in situ depot
title_full_unstemmed Fabrication and in vitro evaluation of pH/thermo dual responsive hydrogels as controlled ibuprofen sodium in situ depot
title_short Fabrication and in vitro evaluation of pH/thermo dual responsive hydrogels as controlled ibuprofen sodium in situ depot
title_sort fabrication and in vitro evaluation of ph thermo dual responsive hydrogels as controlled ibuprofen sodium in situ depot
topic thermogels
poly(n-vinylcaprolactam)
sodium alginate
in situ depot
rheology
mtt assay
url http://dx.doi.org/10.1080/15685551.2024.2442118
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