Cordycepin affects Streptococcus mutans biofilm and interferes with its metabolism
Abstract Background Streptococcus mutans (S. mutans) contributes to caries. The biofilm formed by S. mutans exhibits greater resistance to drugs and host immune defenses than the planktonic form of the bacteria. The objective of this study was to evaluate the anti-biofilm effect of cordycepin from t...
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| Language: | English |
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BMC
2025-01-01
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| Series: | BMC Oral Health |
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| Online Access: | https://doi.org/10.1186/s12903-024-05355-7 |
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| author | Yidan Shao Wenyan Zhu Shanshan Liu Kai Zhang Yu Sun Yudong Liu Tingchi Wen Yingxue Zou Qingwei Zheng |
| author_facet | Yidan Shao Wenyan Zhu Shanshan Liu Kai Zhang Yu Sun Yudong Liu Tingchi Wen Yingxue Zou Qingwei Zheng |
| author_sort | Yidan Shao |
| collection | DOAJ |
| description | Abstract Background Streptococcus mutans (S. mutans) contributes to caries. The biofilm formed by S. mutans exhibits greater resistance to drugs and host immune defenses than the planktonic form of the bacteria. The objective of this study was to evaluate the anti-biofilm effect of cordycepin from the perspective of metabolomics. Methods The minimum inhibitory concentration (MIC) was determined to evaluate the antimicrobial effect of cordycepin on planktonic S. mutans. The 24-h biofilm was treated with 128 µg/mL of cordycepin for 10 min at the 8- or 20-h time points. Biofilm biomass and metabolism were assessed using crystal violet and MTT assays and cordycepin cytotoxicity was evaluated in human oral keratinocytes (HOK) using CCK-8 assays. The live bacterial rate and the biofilm volume were assessed by confocal laser scanning microscopy. Metabolic changes in the biofilm collected at different times during with cordycepin were analyzed by metabolomics and verified by quantitative real-time PCR. Results The results showed that treatment with 128 µg/mL cordycepin reduced both the biomass and metabolic activity of the biofilm without killing the bacteria, and cordycepin at this concentration showed good biocompatibility. Metabolomics analysis showed that differentially abundant metabolites following cordycepin treatment were mainly related to purine and nucleotide metabolism. After immediate treatment with cordycepin, genes related to purine and nucleotide metabolism were downregulated, and the levels of various metabolites changed significantly. However, the effect was reversible. After continuing culture for 4 h, the changes in genes and most metabolites were reversed, although the levels of 2’-deoxyadenosine, 2’-deoxyinosine, and adenine remained significantly different. Conclusions Cordycepin has the effect of anti-biofilm of S. mutans, mainly related to purine and nucleotide metabolism. |
| format | Article |
| id | doaj-art-037a13f061a54a6d8472f85d4913efeb |
| institution | Kabale University |
| issn | 1472-6831 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Oral Health |
| spelling | doaj-art-037a13f061a54a6d8472f85d4913efeb2025-01-05T12:48:22ZengBMCBMC Oral Health1472-68312025-01-0125111710.1186/s12903-024-05355-7Cordycepin affects Streptococcus mutans biofilm and interferes with its metabolismYidan Shao0Wenyan Zhu1Shanshan Liu2Kai Zhang3Yu Sun4Yudong Liu5Tingchi Wen6Yingxue Zou7Qingwei Zheng8Anhui Key Laboratory of Infection and Immunity, Bengbu Medical UniversityAnhui Key Laboratory of Infection and Immunity, Bengbu Medical UniversityAnhui Key Laboratory of Infection and Immunity, Bengbu Medical UniversityDepartment of Stomatology, The First Affiliated Hospital of Bengbu Medical UniversityDepartment of Biochemistry and Molecular Biology, Bengbu Medical UniversityAnhui Key Laboratory of Infection and Immunity, Bengbu Medical UniversityThe Engineering Research Center of Southwest Bio-Pharmaceutical Resources, Ministry of Education, Guizhou UniversityAcademy of Medical Engineering and Transform Medicine, Tianjin UniversityAcademy of Medical Engineering and Transform Medicine, Tianjin UniversityAbstract Background Streptococcus mutans (S. mutans) contributes to caries. The biofilm formed by S. mutans exhibits greater resistance to drugs and host immune defenses than the planktonic form of the bacteria. The objective of this study was to evaluate the anti-biofilm effect of cordycepin from the perspective of metabolomics. Methods The minimum inhibitory concentration (MIC) was determined to evaluate the antimicrobial effect of cordycepin on planktonic S. mutans. The 24-h biofilm was treated with 128 µg/mL of cordycepin for 10 min at the 8- or 20-h time points. Biofilm biomass and metabolism were assessed using crystal violet and MTT assays and cordycepin cytotoxicity was evaluated in human oral keratinocytes (HOK) using CCK-8 assays. The live bacterial rate and the biofilm volume were assessed by confocal laser scanning microscopy. Metabolic changes in the biofilm collected at different times during with cordycepin were analyzed by metabolomics and verified by quantitative real-time PCR. Results The results showed that treatment with 128 µg/mL cordycepin reduced both the biomass and metabolic activity of the biofilm without killing the bacteria, and cordycepin at this concentration showed good biocompatibility. Metabolomics analysis showed that differentially abundant metabolites following cordycepin treatment were mainly related to purine and nucleotide metabolism. After immediate treatment with cordycepin, genes related to purine and nucleotide metabolism were downregulated, and the levels of various metabolites changed significantly. However, the effect was reversible. After continuing culture for 4 h, the changes in genes and most metabolites were reversed, although the levels of 2’-deoxyadenosine, 2’-deoxyinosine, and adenine remained significantly different. Conclusions Cordycepin has the effect of anti-biofilm of S. mutans, mainly related to purine and nucleotide metabolism.https://doi.org/10.1186/s12903-024-05355-7MetabolomicStreptococcus mutansCordycepinBiofilm |
| spellingShingle | Yidan Shao Wenyan Zhu Shanshan Liu Kai Zhang Yu Sun Yudong Liu Tingchi Wen Yingxue Zou Qingwei Zheng Cordycepin affects Streptococcus mutans biofilm and interferes with its metabolism BMC Oral Health Metabolomic Streptococcus mutans Cordycepin Biofilm |
| title | Cordycepin affects Streptococcus mutans biofilm and interferes with its metabolism |
| title_full | Cordycepin affects Streptococcus mutans biofilm and interferes with its metabolism |
| title_fullStr | Cordycepin affects Streptococcus mutans biofilm and interferes with its metabolism |
| title_full_unstemmed | Cordycepin affects Streptococcus mutans biofilm and interferes with its metabolism |
| title_short | Cordycepin affects Streptococcus mutans biofilm and interferes with its metabolism |
| title_sort | cordycepin affects streptococcus mutans biofilm and interferes with its metabolism |
| topic | Metabolomic Streptococcus mutans Cordycepin Biofilm |
| url | https://doi.org/10.1186/s12903-024-05355-7 |
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