Synthesis of Poly[APMA]-DOTA-Cu Conjugates for Interventional Radionuclide Therapy of Prostate Cancer: Assessment of Intratumoral Retention by Micro–Positron Emission Tomography
To develop new radiopharmaceuticals for interventional radionuclide therapy of locally recurrent prostate cancer, poly[ N -(3-aminopropyl)methacrylamide] [poly(APMA)] polymers were synthesized by free radical precipitation polymerization in acetonedimethylsulfoxide using N,N ‘-azobis(isobutyronitril...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2007-01-01
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| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.2310/7290.2006.00030 |
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| _version_ | 1841564627642613760 |
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| author | Jianchao Yuan Yezi You Xin Lu Otto Muzik David Oupicky Fangyu Peng |
| author_facet | Jianchao Yuan Yezi You Xin Lu Otto Muzik David Oupicky Fangyu Peng |
| author_sort | Jianchao Yuan |
| collection | DOAJ |
| description | To develop new radiopharmaceuticals for interventional radionuclide therapy of locally recurrent prostate cancer, poly[ N -(3-aminopropyl)methacrylamide] [poly(APMA)] polymers were synthesized by free radical precipitation polymerization in acetonedimethylsulfoxide using N,N ‘-azobis(isobutyronitrile) as the initiator. The polymers were characterized with nuclear magnetic resonance, size exclusion chromatography, and dynamic light scattering ( M n 5 2.40 × 10 4 , M w / M n = 1.87). Subsequently, poly[APMA] was coupled with 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) using 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride as an activator, followed by conjugation with 64 Cu radionuclide. Prolonged retention of poly[APMA]-DOTA- 64 Cu conjugates within the tumor tissues was demonstrated by micro–positron emission tomography at 24 hours following intra-tumoral injection of the conjugates to human prostate xenografts in mice. The data suggest that the poly[APMA]-DOTA- 64 Cu conjugates might be useful for interventional radionuclide therapy of locally recurrent prostate cancer in humans. |
| format | Article |
| id | doaj-art-02e074b8cfa84664a887dce13ed4dd03 |
| institution | Kabale University |
| issn | 1536-0121 |
| language | English |
| publishDate | 2007-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj-art-02e074b8cfa84664a887dce13ed4dd032025-01-02T22:37:02ZengSAGE PublishingMolecular Imaging1536-01212007-01-01610.2310/7290.2006.0003010.2310_7290.2006.00030Synthesis of Poly[APMA]-DOTA-Cu Conjugates for Interventional Radionuclide Therapy of Prostate Cancer: Assessment of Intratumoral Retention by Micro–Positron Emission TomographyJianchao YuanYezi YouXin LuOtto MuzikDavid OupickyFangyu PengTo develop new radiopharmaceuticals for interventional radionuclide therapy of locally recurrent prostate cancer, poly[ N -(3-aminopropyl)methacrylamide] [poly(APMA)] polymers were synthesized by free radical precipitation polymerization in acetonedimethylsulfoxide using N,N ‘-azobis(isobutyronitrile) as the initiator. The polymers were characterized with nuclear magnetic resonance, size exclusion chromatography, and dynamic light scattering ( M n 5 2.40 × 10 4 , M w / M n = 1.87). Subsequently, poly[APMA] was coupled with 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) using 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride as an activator, followed by conjugation with 64 Cu radionuclide. Prolonged retention of poly[APMA]-DOTA- 64 Cu conjugates within the tumor tissues was demonstrated by micro–positron emission tomography at 24 hours following intra-tumoral injection of the conjugates to human prostate xenografts in mice. The data suggest that the poly[APMA]-DOTA- 64 Cu conjugates might be useful for interventional radionuclide therapy of locally recurrent prostate cancer in humans.https://doi.org/10.2310/7290.2006.00030 |
| spellingShingle | Jianchao Yuan Yezi You Xin Lu Otto Muzik David Oupicky Fangyu Peng Synthesis of Poly[APMA]-DOTA-Cu Conjugates for Interventional Radionuclide Therapy of Prostate Cancer: Assessment of Intratumoral Retention by Micro–Positron Emission Tomography Molecular Imaging |
| title | Synthesis of Poly[APMA]-DOTA-Cu Conjugates for Interventional Radionuclide Therapy of Prostate Cancer: Assessment of Intratumoral Retention by Micro–Positron Emission Tomography |
| title_full | Synthesis of Poly[APMA]-DOTA-Cu Conjugates for Interventional Radionuclide Therapy of Prostate Cancer: Assessment of Intratumoral Retention by Micro–Positron Emission Tomography |
| title_fullStr | Synthesis of Poly[APMA]-DOTA-Cu Conjugates for Interventional Radionuclide Therapy of Prostate Cancer: Assessment of Intratumoral Retention by Micro–Positron Emission Tomography |
| title_full_unstemmed | Synthesis of Poly[APMA]-DOTA-Cu Conjugates for Interventional Radionuclide Therapy of Prostate Cancer: Assessment of Intratumoral Retention by Micro–Positron Emission Tomography |
| title_short | Synthesis of Poly[APMA]-DOTA-Cu Conjugates for Interventional Radionuclide Therapy of Prostate Cancer: Assessment of Intratumoral Retention by Micro–Positron Emission Tomography |
| title_sort | synthesis of poly apma dota cu conjugates for interventional radionuclide therapy of prostate cancer assessment of intratumoral retention by micro positron emission tomography |
| url | https://doi.org/10.2310/7290.2006.00030 |
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