Development of collagenous scaffolds for wound healing: characterization and in vivo analysis
Abstract The development of wound dressings from biomaterials has been the subject of research due to their unique structural and functional characteristics. Proteins from animal origin, such as collagen and chitosan, act as promising materials for applications in injuries and chronic wounds, functi...
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| Format: | Article |
| Language: | English |
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Springer
2024-02-01
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| Series: | Journal of Materials Science: Materials in Medicine |
| Online Access: | https://doi.org/10.1007/s10856-023-06774-8 |
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| author | Jéssica Peixoto Rodrigues Jéssica Regina da Costa Silva Bruno Antônio Ferreira Lucas Ian Veloso Ludmila Sousa Quirino Roberta Rezende Rosa Matheus Carvalho Barbosa Cláudia Mendonça Rodrigues Paula Batista Fernandes Gaspari Marcelo Emílio Beletti Luiz Ricardo Goulart Natássia Caroline Resende Corrêa |
| author_facet | Jéssica Peixoto Rodrigues Jéssica Regina da Costa Silva Bruno Antônio Ferreira Lucas Ian Veloso Ludmila Sousa Quirino Roberta Rezende Rosa Matheus Carvalho Barbosa Cláudia Mendonça Rodrigues Paula Batista Fernandes Gaspari Marcelo Emílio Beletti Luiz Ricardo Goulart Natássia Caroline Resende Corrêa |
| author_sort | Jéssica Peixoto Rodrigues |
| collection | DOAJ |
| description | Abstract The development of wound dressings from biomaterials has been the subject of research due to their unique structural and functional characteristics. Proteins from animal origin, such as collagen and chitosan, act as promising materials for applications in injuries and chronic wounds, functioning as a repairing agent. This study aims to evaluate in vitro effects of scaffolds with different formulations containing bioactive compounds such as collagen, chitosan, N-acetylcysteine (NAC) and ε-poly-lysine (ε-PL). We manufactured a scaffold made of a collagen hydrogel bioconjugated with chitosan by crosslinking and addition of NAC and ε-PL. Cell viability was verified by resazurin and live/dead assays and the ultrastructure of biomaterials was evaluated by SEM. Antimicrobial sensitivity was assessed by antibiogram. The healing potential of the biomaterial was evaluated in vivo, in a model of healing of excisional wounds in mice. On the 7th day after the injury, the wounds and surrounding skin were processed for evaluation of biochemical and histological parameters associated with the inflammatory process. The results showed great cell viability and increase in porosity after crosslinking while antimicrobial action was observed in scaffolds containing NAC and ε-PL. Chitosan scaffolds bioconjugated with NAC/ε-PL showed improvement in tissue healing, with reduced lesion size and reduced inflammation. It is concluded that scaffolds crosslinked with chitosan-NAC-ε-PL have the desirable characteristics for tissue repair at low cost and could be considered promising biomaterials in the practice of regenerative medicine. Graphical Abstract |
| format | Article |
| id | doaj-art-02c23eab20f04330a01caa3b67fae0b9 |
| institution | Kabale University |
| issn | 1573-4838 |
| language | English |
| publishDate | 2024-02-01 |
| publisher | Springer |
| record_format | Article |
| series | Journal of Materials Science: Materials in Medicine |
| spelling | doaj-art-02c23eab20f04330a01caa3b67fae0b92024-12-22T12:11:05ZengSpringerJournal of Materials Science: Materials in Medicine1573-48382024-02-0135111210.1007/s10856-023-06774-8Development of collagenous scaffolds for wound healing: characterization and in vivo analysisJéssica Peixoto Rodrigues0Jéssica Regina da Costa Silva1Bruno Antônio Ferreira2Lucas Ian Veloso3Ludmila Sousa Quirino4Roberta Rezende Rosa5Matheus Carvalho Barbosa6Cláudia Mendonça Rodrigues7Paula Batista Fernandes Gaspari8Marcelo Emílio Beletti9Luiz Ricardo Goulart10Natássia Caroline Resende Corrêa11Laboratory of Nanobiotechnology, Institute of Biotechnology, Federal University of UberlandiaLaboratory of Nanobiotechnology, Institute of Biotechnology, Federal University of UberlandiaDepartment of Physiological Sciences, Federal University of Uberlândia, UFULaboratory of Nanobiotechnology, Institute of Biotechnology, Federal University of UberlandiaLaboratory of Nanobiotechnology, Institute of Biotechnology, Federal University of UberlandiaLaboratory of Nanobiotechnology, Institute of Biotechnology, Federal University of UberlandiaLaboratory of Nanobiotechnology, Institute of Biotechnology, Federal University of UberlandiaLaboratory of Nanobiotechnology, Institute of Biotechnology, Federal University of UberlandiaLaboratory of Nanobiotechnology, Institute of Biotechnology, Federal University of UberlandiaDepartment of Cell Biology, Histology and Embryology, Federal University of Uberlândia, UFULaboratory of Nanobiotechnology, Institute of Biotechnology, Federal University of UberlandiaLaboratory of Nanobiotechnology, Institute of Biotechnology, Federal University of UberlandiaAbstract The development of wound dressings from biomaterials has been the subject of research due to their unique structural and functional characteristics. Proteins from animal origin, such as collagen and chitosan, act as promising materials for applications in injuries and chronic wounds, functioning as a repairing agent. This study aims to evaluate in vitro effects of scaffolds with different formulations containing bioactive compounds such as collagen, chitosan, N-acetylcysteine (NAC) and ε-poly-lysine (ε-PL). We manufactured a scaffold made of a collagen hydrogel bioconjugated with chitosan by crosslinking and addition of NAC and ε-PL. Cell viability was verified by resazurin and live/dead assays and the ultrastructure of biomaterials was evaluated by SEM. Antimicrobial sensitivity was assessed by antibiogram. The healing potential of the biomaterial was evaluated in vivo, in a model of healing of excisional wounds in mice. On the 7th day after the injury, the wounds and surrounding skin were processed for evaluation of biochemical and histological parameters associated with the inflammatory process. The results showed great cell viability and increase in porosity after crosslinking while antimicrobial action was observed in scaffolds containing NAC and ε-PL. Chitosan scaffolds bioconjugated with NAC/ε-PL showed improvement in tissue healing, with reduced lesion size and reduced inflammation. It is concluded that scaffolds crosslinked with chitosan-NAC-ε-PL have the desirable characteristics for tissue repair at low cost and could be considered promising biomaterials in the practice of regenerative medicine. Graphical Abstracthttps://doi.org/10.1007/s10856-023-06774-8 |
| spellingShingle | Jéssica Peixoto Rodrigues Jéssica Regina da Costa Silva Bruno Antônio Ferreira Lucas Ian Veloso Ludmila Sousa Quirino Roberta Rezende Rosa Matheus Carvalho Barbosa Cláudia Mendonça Rodrigues Paula Batista Fernandes Gaspari Marcelo Emílio Beletti Luiz Ricardo Goulart Natássia Caroline Resende Corrêa Development of collagenous scaffolds for wound healing: characterization and in vivo analysis Journal of Materials Science: Materials in Medicine |
| title | Development of collagenous scaffolds for wound healing: characterization and in vivo analysis |
| title_full | Development of collagenous scaffolds for wound healing: characterization and in vivo analysis |
| title_fullStr | Development of collagenous scaffolds for wound healing: characterization and in vivo analysis |
| title_full_unstemmed | Development of collagenous scaffolds for wound healing: characterization and in vivo analysis |
| title_short | Development of collagenous scaffolds for wound healing: characterization and in vivo analysis |
| title_sort | development of collagenous scaffolds for wound healing characterization and in vivo analysis |
| url | https://doi.org/10.1007/s10856-023-06774-8 |
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