Expression of Long Non-Coding RNA H19 and miR-675 in Patients with Colorectal Cancer and Ulcerative Colitis
Background: Colorectal cancer (CRC), which has a high mortality rate, is one of the most common malignancies. It is a growing public health problem that recently encompasses the younger age group. Inflammatory bowel disease (IBD), like (UC), is a risk factor for CRC. The aim of this study was to tes...
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International Medical Research and Development Corporation
2024-12-01
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| Series: | International Journal of Biomedicine |
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| Online Access: | http://www.ijbm.org/articles/i56/ijbm_14(4)_oa6.pdf |
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| author | Rania Sobhy Abu Taleb Olfat Shaker Ahmed Khairy Dalia A. Gaber |
| author_facet | Rania Sobhy Abu Taleb Olfat Shaker Ahmed Khairy Dalia A. Gaber |
| author_sort | Rania Sobhy Abu Taleb |
| collection | DOAJ |
| description | Background: Colorectal cancer (CRC), which has a high mortality rate, is one of the most common malignancies. It is a growing public health problem that recently encompasses the younger age group. Inflammatory bowel disease (IBD), like (UC), is a risk factor for CRC. The aim of this study was to test the expression of microRNA-675 (miR-675) and long noncoding RNA H19 (H19) for their diagnostic and prognostic potential in CRC and UC.
Methods and Results: This prospective cross-sectional study included 60 patients diagnosed with CRC, 60 patients with UC, and 30 healthy subjects as controls. The real-time RT-PCR technique quantified the expressions of the non-coding RNAs (ncRNAs) miR-675 and lncH19. The biochemical and radiological findings were assessed and correlated with the expression of the genetic biomarkers.
H19 showed high expression in both CRC and UC groups, which is significantly different from the control group. There was a significantly enhanced expression of H19 in CRC, compared to the UC group. Increased H19 expression was detected in the caecum and ascending colonic lesions, with a significant difference from flexures and transverse colon (P=0.010), and recto-sigmoid colon (P=0.05). Although miR-675 showed downregulation in both UC and CRC groups, it revealed a higher expression in CRC when compared to the UC group.
Conclusion: Based on our findings, we can conclude that H19 can be a potential noninvasive biomarker for the diagnosis of UC and early detection of CRC. |
| format | Article |
| id | doaj-art-02c1262590154d76a9fea8c33ccb1563 |
| institution | Kabale University |
| issn | 2158-0510 2158-0529 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | International Medical Research and Development Corporation |
| record_format | Article |
| series | International Journal of Biomedicine |
| spelling | doaj-art-02c1262590154d76a9fea8c33ccb15632024-12-07T01:25:50ZengInternational Medical Research and Development CorporationInternational Journal of Biomedicine2158-05102158-05292024-12-0114457558210.21103/Article14(4)_OA6Expression of Long Non-Coding RNA H19 and miR-675 in Patients with Colorectal Cancer and Ulcerative Colitis Rania Sobhy Abu Taleb0Olfat Shaker1Ahmed Khairy2Dalia A. Gaber3Medical Biochemistry & Molecular Biology Department, Faculty of Medicine, Helwan University, Cairo, EgyptMedical Biochemistry & Molecular Biology Department, Faculty of Medicine, Cairo University, Egypt Endemic Medicine Department, Faculty of Medicine Kasr Al-Ainy, Cairo University, EgyptEndemic Medicine Department, Faculty of Medicine Kasr Al-Ainy, Cairo University, Egypt; College of Medicine, Gulf Medical University, Ajman, United Arab EmiratesBackground: Colorectal cancer (CRC), which has a high mortality rate, is one of the most common malignancies. It is a growing public health problem that recently encompasses the younger age group. Inflammatory bowel disease (IBD), like (UC), is a risk factor for CRC. The aim of this study was to test the expression of microRNA-675 (miR-675) and long noncoding RNA H19 (H19) for their diagnostic and prognostic potential in CRC and UC. Methods and Results: This prospective cross-sectional study included 60 patients diagnosed with CRC, 60 patients with UC, and 30 healthy subjects as controls. The real-time RT-PCR technique quantified the expressions of the non-coding RNAs (ncRNAs) miR-675 and lncH19. The biochemical and radiological findings were assessed and correlated with the expression of the genetic biomarkers. H19 showed high expression in both CRC and UC groups, which is significantly different from the control group. There was a significantly enhanced expression of H19 in CRC, compared to the UC group. Increased H19 expression was detected in the caecum and ascending colonic lesions, with a significant difference from flexures and transverse colon (P=0.010), and recto-sigmoid colon (P=0.05). Although miR-675 showed downregulation in both UC and CRC groups, it revealed a higher expression in CRC when compared to the UC group. Conclusion: Based on our findings, we can conclude that H19 can be a potential noninvasive biomarker for the diagnosis of UC and early detection of CRC.http://www.ijbm.org/articles/i56/ijbm_14(4)_oa6.pdflnch19mir-675colorectal cancerulcerative colitis |
| spellingShingle | Rania Sobhy Abu Taleb Olfat Shaker Ahmed Khairy Dalia A. Gaber Expression of Long Non-Coding RNA H19 and miR-675 in Patients with Colorectal Cancer and Ulcerative Colitis International Journal of Biomedicine lnch19 mir-675 colorectal cancer ulcerative colitis |
| title | Expression of Long Non-Coding RNA H19 and miR-675 in Patients with Colorectal Cancer and Ulcerative Colitis |
| title_full | Expression of Long Non-Coding RNA H19 and miR-675 in Patients with Colorectal Cancer and Ulcerative Colitis |
| title_fullStr | Expression of Long Non-Coding RNA H19 and miR-675 in Patients with Colorectal Cancer and Ulcerative Colitis |
| title_full_unstemmed | Expression of Long Non-Coding RNA H19 and miR-675 in Patients with Colorectal Cancer and Ulcerative Colitis |
| title_short | Expression of Long Non-Coding RNA H19 and miR-675 in Patients with Colorectal Cancer and Ulcerative Colitis |
| title_sort | expression of long non coding rna h19 and mir 675 in patients with colorectal cancer and ulcerative colitis |
| topic | lnch19 mir-675 colorectal cancer ulcerative colitis |
| url | http://www.ijbm.org/articles/i56/ijbm_14(4)_oa6.pdf |
| work_keys_str_mv | AT raniasobhyabutaleb expressionoflongnoncodingrnah19andmir675inpatientswithcolorectalcancerandulcerativecolitis AT olfatshaker expressionoflongnoncodingrnah19andmir675inpatientswithcolorectalcancerandulcerativecolitis AT ahmedkhairy expressionoflongnoncodingrnah19andmir675inpatientswithcolorectalcancerandulcerativecolitis AT daliaagaber expressionoflongnoncodingrnah19andmir675inpatientswithcolorectalcancerandulcerativecolitis |