Effect of Low-temperature Aqueous Extraction on Flavor, Functional Ingredients and in Vitro Bioactivities of Penthorum chinense Pursh Leaf Extracts

Penthorum chinense Pursh (PCP) leaf was a new food material for botanical beverage production, with bioactivities of antioxidant, hepatoprotective and anti-alcoholism but showing unpleasant flavors, especially for the high-temperature aqueous extracts. Accordingly, to reduce its unfavorable flavor w...

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Main Authors: Yijun LIU, Wei XU, Xue TIAN, Xinyu LIU, Yongqing TANG, Xuan LIU, Qian LAI, Li ZENG, Gang LI, Feixia DUAN
Format: Article
Language:zho
Published: The editorial department of Science and Technology of Food Industry 2025-08-01
Series:Shipin gongye ke-ji
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Online Access:http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024080225
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author Yijun LIU
Wei XU
Xue TIAN
Xinyu LIU
Yongqing TANG
Xuan LIU
Qian LAI
Li ZENG
Gang LI
Feixia DUAN
author_facet Yijun LIU
Wei XU
Xue TIAN
Xinyu LIU
Yongqing TANG
Xuan LIU
Qian LAI
Li ZENG
Gang LI
Feixia DUAN
author_sort Yijun LIU
collection DOAJ
description Penthorum chinense Pursh (PCP) leaf was a new food material for botanical beverage production, with bioactivities of antioxidant, hepatoprotective and anti-alcoholism but showing unpleasant flavors, especially for the high-temperature aqueous extracts. Accordingly, to reduce its unfavorable flavor with no effect on its health benefiting, the low-temperature aqueous extract (PLE50) was prepared by optimal low-temperature extraction. With the maximum aqueous solubility at 14.2 mg/mL, PLE50 showed the concentrations of phenols, flavonoids, gallic acid, quercetin, and pinocembrin at 1.90, 1.41 mg/mL, 49.98, 23.57 and 11.93 μg/mL, respectively, being significantly (P<0.05) higher than water brewed PCP soup. The results of HS-SPME-GC-MS revealed that safranal and 2,4-ditert-butylphenol were the primary sources of the unfavorable flavors in the boiling water extract of PCP leaves (PLE95), which contributed 54.43% and 5.89% to the overall flavor, respectively. Conversely, PLE50 showed little contents of safranal and 2,4-ditert-butylphenol with flavor contribution less than 0.1%. The major aroma components of PLE50 include decanal, β-cyclocitral, linalool, β-ionone, undecanal, 2-undecanone, 2-decanone, D-limonene, and dodecanal, contributing 76.97% to its flavor, which significantly (P<0.05) improved the sensory acceptability of PLE50. Besides, PLE50 showed the half scavenging concentrations for DPPH, ABTS+, and hydroxyl radicals at 0.034, 0.087, and 0.91 mg/mL, respectively, with 2.26 mmol/L ferric ion reducing power at the concentration of 1.6 mg/mL. PLE50 at 14.2 mg/mL exhibited the maximum activation rates at 183.33% for alcohol dehydrogenase and 951.88% for aldehyde dehydrogenase, being better than PLE95 at the same concentration. In summary, the optimal low-temperature aqueous extraction improved the flavor and sensory acceptability of PCP extracts, and maintained its in vitro antioxidant activity, as well as its activation capabilities for alcohol dehydrogenase and aldehyde dehydrogenase.
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publisher The editorial department of Science and Technology of Food Industry
record_format Article
series Shipin gongye ke-ji
spelling doaj-art-02a47a8b085d41f2bf24b70ab65aca6f2025-08-20T03:47:03ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062025-08-01461622123410.13386/j.issn1002-0306.20240802252024080225-16Effect of Low-temperature Aqueous Extraction on Flavor, Functional Ingredients and in Vitro Bioactivities of Penthorum chinense Pursh Leaf ExtractsYijun LIU0Wei XU1Xue TIAN2Xinyu LIU3Yongqing TANG4Xuan LIU5Qian LAI6Li ZENG7Gang LI8Feixia DUAN9College of Biomass Science and Engineering, Sichuan University, Chengdu 610065, ChinaCollege of Biomass Science and Engineering, Sichuan University, Chengdu 610065, ChinaCollege of Biomass Science and Engineering, Sichuan University, Chengdu 610065, ChinaCollege of Biomass Science and Engineering, Sichuan University, Chengdu 610065, ChinaChengdu-Chongqing Economic Circle (Luzhou) Advanced Technology Research Institute, Luzhou 646000, ChinaChengdu-Chongqing Economic Circle (Luzhou) Advanced Technology Research Institute, Luzhou 646000, ChinaAgriculture and Rural Bureau of Gulin County, Luzhou 646500, ChinaCollege of Biomass Science and Engineering, Sichuan University, Chengdu 610065, ChinaGucui Biotechnology Co., Ltd., Luzhou 646500, ChinaCollege of Biomass Science and Engineering, Sichuan University, Chengdu 610065, ChinaPenthorum chinense Pursh (PCP) leaf was a new food material for botanical beverage production, with bioactivities of antioxidant, hepatoprotective and anti-alcoholism but showing unpleasant flavors, especially for the high-temperature aqueous extracts. Accordingly, to reduce its unfavorable flavor with no effect on its health benefiting, the low-temperature aqueous extract (PLE50) was prepared by optimal low-temperature extraction. With the maximum aqueous solubility at 14.2 mg/mL, PLE50 showed the concentrations of phenols, flavonoids, gallic acid, quercetin, and pinocembrin at 1.90, 1.41 mg/mL, 49.98, 23.57 and 11.93 μg/mL, respectively, being significantly (P<0.05) higher than water brewed PCP soup. The results of HS-SPME-GC-MS revealed that safranal and 2,4-ditert-butylphenol were the primary sources of the unfavorable flavors in the boiling water extract of PCP leaves (PLE95), which contributed 54.43% and 5.89% to the overall flavor, respectively. Conversely, PLE50 showed little contents of safranal and 2,4-ditert-butylphenol with flavor contribution less than 0.1%. The major aroma components of PLE50 include decanal, β-cyclocitral, linalool, β-ionone, undecanal, 2-undecanone, 2-decanone, D-limonene, and dodecanal, contributing 76.97% to its flavor, which significantly (P<0.05) improved the sensory acceptability of PLE50. Besides, PLE50 showed the half scavenging concentrations for DPPH, ABTS+, and hydroxyl radicals at 0.034, 0.087, and 0.91 mg/mL, respectively, with 2.26 mmol/L ferric ion reducing power at the concentration of 1.6 mg/mL. PLE50 at 14.2 mg/mL exhibited the maximum activation rates at 183.33% for alcohol dehydrogenase and 951.88% for aldehyde dehydrogenase, being better than PLE95 at the same concentration. In summary, the optimal low-temperature aqueous extraction improved the flavor and sensory acceptability of PCP extracts, and maintained its in vitro antioxidant activity, as well as its activation capabilities for alcohol dehydrogenase and aldehyde dehydrogenase.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024080225penthorum chinense pursh leaveslow-temperature aqueous extractionflavor componentsantioxidant activityanti-alcoholism capability in vitro
spellingShingle Yijun LIU
Wei XU
Xue TIAN
Xinyu LIU
Yongqing TANG
Xuan LIU
Qian LAI
Li ZENG
Gang LI
Feixia DUAN
Effect of Low-temperature Aqueous Extraction on Flavor, Functional Ingredients and in Vitro Bioactivities of Penthorum chinense Pursh Leaf Extracts
Shipin gongye ke-ji
penthorum chinense pursh leaves
low-temperature aqueous extraction
flavor components
antioxidant activity
anti-alcoholism capability in vitro
title Effect of Low-temperature Aqueous Extraction on Flavor, Functional Ingredients and in Vitro Bioactivities of Penthorum chinense Pursh Leaf Extracts
title_full Effect of Low-temperature Aqueous Extraction on Flavor, Functional Ingredients and in Vitro Bioactivities of Penthorum chinense Pursh Leaf Extracts
title_fullStr Effect of Low-temperature Aqueous Extraction on Flavor, Functional Ingredients and in Vitro Bioactivities of Penthorum chinense Pursh Leaf Extracts
title_full_unstemmed Effect of Low-temperature Aqueous Extraction on Flavor, Functional Ingredients and in Vitro Bioactivities of Penthorum chinense Pursh Leaf Extracts
title_short Effect of Low-temperature Aqueous Extraction on Flavor, Functional Ingredients and in Vitro Bioactivities of Penthorum chinense Pursh Leaf Extracts
title_sort effect of low temperature aqueous extraction on flavor functional ingredients and in vitro bioactivities of penthorum chinense pursh leaf extracts
topic penthorum chinense pursh leaves
low-temperature aqueous extraction
flavor components
antioxidant activity
anti-alcoholism capability in vitro
url http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024080225
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