Phylogenetic analysis of the complete mitochondrial genome of the orange-winged sulphur butterfly Dercas nina Mell 1913 (Insecta: Lepidoptera: Pieridae: Coliadinae)
Dercas nina Mell 1913 (Pieridae) is a little-studied butterfly species endemic to China that flies primarily in the forest canopy. Genome skimming by Illumina sequencing allowed assembly of 146,702 reads for complete 1471.3-fold mean coverage of the circular 15,264 bp mitogenome from D. nina consist...
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Taylor & Francis Group
2024-11-01
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| Series: | Mitochondrial DNA. Part B. Resources |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/23802359.2024.2427109 |
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| author | Arlene M. Agcaoili Nabiha Ameena Dexter Andres Rhey Caners Manishvinder K. Chahal Nicole J. Croitor Gabrielle M. David Kaesy L. Enns Oleksandra Fedorova Hannah A. Garber Sarah D. Gregoire Tenley E. Ilnisky Annie Jiang Anthony Kozak Feryal Ladha Alexandria Martin Mary A. McAuley Liam R. McEachern Cassidy McNeill Senudi D. Nanayakkara Ngoc Thao Vy Nguyen Gaeun Park Deanna K. Peters Madison N. Poitras Jolene Potts Dhruvi V. Prajapati Camille D. Prefontaine Ravindu V. Rajapaksha Pratyaksh Singhal Cedey Souriyavong Colby Stoker Kayla R. Talabis Yantong Tan Jasmin L. Tang Kailey W. Tkach Ashley J. Tohms Cameron G. Tramley Josh Treftlin Diya Ukani Ethan A. Vallelly Patrick V. Wiens Carissa Yee Ke Yu Jeffrey M. Marcus |
| author_facet | Arlene M. Agcaoili Nabiha Ameena Dexter Andres Rhey Caners Manishvinder K. Chahal Nicole J. Croitor Gabrielle M. David Kaesy L. Enns Oleksandra Fedorova Hannah A. Garber Sarah D. Gregoire Tenley E. Ilnisky Annie Jiang Anthony Kozak Feryal Ladha Alexandria Martin Mary A. McAuley Liam R. McEachern Cassidy McNeill Senudi D. Nanayakkara Ngoc Thao Vy Nguyen Gaeun Park Deanna K. Peters Madison N. Poitras Jolene Potts Dhruvi V. Prajapati Camille D. Prefontaine Ravindu V. Rajapaksha Pratyaksh Singhal Cedey Souriyavong Colby Stoker Kayla R. Talabis Yantong Tan Jasmin L. Tang Kailey W. Tkach Ashley J. Tohms Cameron G. Tramley Josh Treftlin Diya Ukani Ethan A. Vallelly Patrick V. Wiens Carissa Yee Ke Yu Jeffrey M. Marcus |
| author_sort | Arlene M. Agcaoili |
| collection | DOAJ |
| description | Dercas nina Mell 1913 (Pieridae) is a little-studied butterfly species endemic to China that flies primarily in the forest canopy. Genome skimming by Illumina sequencing allowed assembly of 146,702 reads for complete 1471.3-fold mean coverage of the circular 15,264 bp mitogenome from D. nina consisting of 82.1% AT nucleotides. A gene order typical of butterflies was recovered consisting of 13 protein-coding genes, 22 tRNAs, two rRNAs, and a predicted control region. The Dercas nina COX1 open reading frame begins with atypical start codon CGA. Six protein-coding genes (COX1, COX2, ND2, ND3, ND4, ND5) with single-nucleotide (T) stop codons, and two protein-coding genes (ATP6, ATP8) with two-nucleotide (TA) stop codons encoded in the DNA were inferred to be completed by adenine nucleotides from the Poly-A tail of the mRNA. Bayesian’s phylogenetic reconstruction places the D. nina and D. lycorias mitogenomes as sister clades. Dercas mitogenomes were sister to those from genus Colias in the monophyletic subfamily Coliadinae. The mitogenome phylogeny is consistent with previous molecular phylogenetic hypotheses based on other markers, but differs somewhat from a morphology-based hypothesis that suggested that Dercas was more closely related to genus Gonepteryx. This may falsify the hypothesis or may instead reflect mitochondrial-nuclear phylogenetic discordance. |
| format | Article |
| id | doaj-art-02a45b5512b14ee790963f0e1b706f3c |
| institution | Kabale University |
| issn | 2380-2359 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Mitochondrial DNA. Part B. Resources |
| spelling | doaj-art-02a45b5512b14ee790963f0e1b706f3c2024-12-23T06:12:38ZengTaylor & Francis GroupMitochondrial DNA. Part B. Resources2380-23592024-11-019111510151710.1080/23802359.2024.2427109Phylogenetic analysis of the complete mitochondrial genome of the orange-winged sulphur butterfly Dercas nina Mell 1913 (Insecta: Lepidoptera: Pieridae: Coliadinae)Arlene M. Agcaoili0Nabiha Ameena1Dexter Andres2Rhey Caners3Manishvinder K. Chahal4Nicole J. Croitor5Gabrielle M. David6Kaesy L. Enns7Oleksandra Fedorova8Hannah A. Garber9Sarah D. Gregoire10Tenley E. Ilnisky11Annie Jiang12Anthony Kozak13Feryal Ladha14Alexandria Martin15Mary A. McAuley16Liam R. McEachern17Cassidy McNeill18Senudi D. Nanayakkara19Ngoc Thao Vy Nguyen20Gaeun Park21Deanna K. Peters22Madison N. Poitras23Jolene Potts24Dhruvi V. Prajapati25Camille D. Prefontaine26Ravindu V. Rajapaksha27Pratyaksh Singhal28Cedey Souriyavong29Colby Stoker30Kayla R. Talabis31Yantong Tan32Jasmin L. Tang33Kailey W. Tkach34Ashley J. Tohms35Cameron G. Tramley36Josh Treftlin37Diya Ukani38Ethan A. Vallelly39Patrick V. Wiens40Carissa Yee41Ke Yu42Jeffrey M. Marcus43Department of Biological Sciences, University of Manitoba, Winnipeg, CanadaDepartment of Biological Sciences, University of Manitoba, Winnipeg, CanadaDepartment of Biological Sciences, University of Manitoba, Winnipeg, CanadaDepartment of Biological Sciences, University of Manitoba, Winnipeg, CanadaDepartment of Biological Sciences, University of Manitoba, Winnipeg, CanadaDepartment of Biological Sciences, University of Manitoba, Winnipeg, CanadaDepartment of Biological Sciences, University of Manitoba, Winnipeg, CanadaDepartment of Biological Sciences, University of Manitoba, Winnipeg, CanadaDepartment of Biological Sciences, University of Manitoba, Winnipeg, CanadaDepartment of Biological Sciences, University of Manitoba, Winnipeg, CanadaDepartment of Biological Sciences, University of Manitoba, Winnipeg, CanadaDepartment of Biological Sciences, University of Manitoba, Winnipeg, CanadaDepartment of Biological Sciences, University of Manitoba, Winnipeg, CanadaDepartment of Biological Sciences, University of Manitoba, Winnipeg, CanadaDepartment of Biological Sciences, University of Manitoba, Winnipeg, CanadaDepartment of Biological Sciences, University of Manitoba, Winnipeg, CanadaDepartment of Biological Sciences, University of Manitoba, Winnipeg, CanadaDepartment of Biological Sciences, University of Manitoba, Winnipeg, CanadaDepartment of Biological Sciences, University of Manitoba, Winnipeg, CanadaDepartment of Biological Sciences, University of Manitoba, Winnipeg, CanadaDepartment of Biological Sciences, University of Manitoba, Winnipeg, CanadaDepartment of Biological Sciences, University of Manitoba, Winnipeg, CanadaDepartment of Biological Sciences, University of Manitoba, Winnipeg, CanadaDepartment of Biological Sciences, University of Manitoba, Winnipeg, CanadaDepartment of Biological Sciences, University of Manitoba, Winnipeg, CanadaDepartment of Biological Sciences, University of Manitoba, Winnipeg, CanadaDepartment of Biological Sciences, University of Manitoba, Winnipeg, CanadaDepartment of Biological Sciences, University of Manitoba, Winnipeg, CanadaDepartment of Biological Sciences, University of Manitoba, Winnipeg, CanadaDepartment of Biological Sciences, University of Manitoba, Winnipeg, CanadaDepartment of Biological Sciences, University of Manitoba, Winnipeg, CanadaDepartment of Biological Sciences, University of Manitoba, Winnipeg, CanadaDepartment of Biological Sciences, University of Manitoba, Winnipeg, CanadaDepartment of Biological Sciences, University of Manitoba, Winnipeg, CanadaDepartment of Biological Sciences, University of Manitoba, Winnipeg, CanadaDepartment of Biological Sciences, University of Manitoba, Winnipeg, CanadaDepartment of Biological Sciences, University of Manitoba, Winnipeg, CanadaDepartment of Biological Sciences, University of Manitoba, Winnipeg, CanadaDepartment of Biological Sciences, University of Manitoba, Winnipeg, CanadaDepartment of Biological Sciences, University of Manitoba, Winnipeg, CanadaDepartment of Biological Sciences, University of Manitoba, Winnipeg, CanadaDepartment of Biological Sciences, University of Manitoba, Winnipeg, CanadaDepartment of Biological Sciences, University of Manitoba, Winnipeg, CanadaDepartment of Biological Sciences, University of Manitoba, Winnipeg, CanadaDercas nina Mell 1913 (Pieridae) is a little-studied butterfly species endemic to China that flies primarily in the forest canopy. Genome skimming by Illumina sequencing allowed assembly of 146,702 reads for complete 1471.3-fold mean coverage of the circular 15,264 bp mitogenome from D. nina consisting of 82.1% AT nucleotides. A gene order typical of butterflies was recovered consisting of 13 protein-coding genes, 22 tRNAs, two rRNAs, and a predicted control region. The Dercas nina COX1 open reading frame begins with atypical start codon CGA. Six protein-coding genes (COX1, COX2, ND2, ND3, ND4, ND5) with single-nucleotide (T) stop codons, and two protein-coding genes (ATP6, ATP8) with two-nucleotide (TA) stop codons encoded in the DNA were inferred to be completed by adenine nucleotides from the Poly-A tail of the mRNA. Bayesian’s phylogenetic reconstruction places the D. nina and D. lycorias mitogenomes as sister clades. Dercas mitogenomes were sister to those from genus Colias in the monophyletic subfamily Coliadinae. The mitogenome phylogeny is consistent with previous molecular phylogenetic hypotheses based on other markers, but differs somewhat from a morphology-based hypothesis that suggested that Dercas was more closely related to genus Gonepteryx. This may falsify the hypothesis or may instead reflect mitochondrial-nuclear phylogenetic discordance.https://www.tandfonline.com/doi/10.1080/23802359.2024.2427109Illumina sequencingmitogenomicsgenome skimming |
| spellingShingle | Arlene M. Agcaoili Nabiha Ameena Dexter Andres Rhey Caners Manishvinder K. Chahal Nicole J. Croitor Gabrielle M. David Kaesy L. Enns Oleksandra Fedorova Hannah A. Garber Sarah D. Gregoire Tenley E. Ilnisky Annie Jiang Anthony Kozak Feryal Ladha Alexandria Martin Mary A. McAuley Liam R. McEachern Cassidy McNeill Senudi D. Nanayakkara Ngoc Thao Vy Nguyen Gaeun Park Deanna K. Peters Madison N. Poitras Jolene Potts Dhruvi V. Prajapati Camille D. Prefontaine Ravindu V. Rajapaksha Pratyaksh Singhal Cedey Souriyavong Colby Stoker Kayla R. Talabis Yantong Tan Jasmin L. Tang Kailey W. Tkach Ashley J. Tohms Cameron G. Tramley Josh Treftlin Diya Ukani Ethan A. Vallelly Patrick V. Wiens Carissa Yee Ke Yu Jeffrey M. Marcus Phylogenetic analysis of the complete mitochondrial genome of the orange-winged sulphur butterfly Dercas nina Mell 1913 (Insecta: Lepidoptera: Pieridae: Coliadinae) Mitochondrial DNA. Part B. Resources Illumina sequencing mitogenomics genome skimming |
| title | Phylogenetic analysis of the complete mitochondrial genome of the orange-winged sulphur butterfly Dercas nina Mell 1913 (Insecta: Lepidoptera: Pieridae: Coliadinae) |
| title_full | Phylogenetic analysis of the complete mitochondrial genome of the orange-winged sulphur butterfly Dercas nina Mell 1913 (Insecta: Lepidoptera: Pieridae: Coliadinae) |
| title_fullStr | Phylogenetic analysis of the complete mitochondrial genome of the orange-winged sulphur butterfly Dercas nina Mell 1913 (Insecta: Lepidoptera: Pieridae: Coliadinae) |
| title_full_unstemmed | Phylogenetic analysis of the complete mitochondrial genome of the orange-winged sulphur butterfly Dercas nina Mell 1913 (Insecta: Lepidoptera: Pieridae: Coliadinae) |
| title_short | Phylogenetic analysis of the complete mitochondrial genome of the orange-winged sulphur butterfly Dercas nina Mell 1913 (Insecta: Lepidoptera: Pieridae: Coliadinae) |
| title_sort | phylogenetic analysis of the complete mitochondrial genome of the orange winged sulphur butterfly dercas nina mell 1913 insecta lepidoptera pieridae coliadinae |
| topic | Illumina sequencing mitogenomics genome skimming |
| url | https://www.tandfonline.com/doi/10.1080/23802359.2024.2427109 |
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