Rumen fluid from donor cows fed different additives can affect the in vitro fermentation parameters
This study assessed the impact of rumen ‘fluid not adapted’ (FNA) and of rumen ‘fluid adapted’ (FA) to three additives on in vitro kinetics of gas production and end products of fermentation. The experiment was performed according to a Latin Square Design of 4 cows and 4 experimental periods. The dr...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Italian Journal of Animal Science |
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| Online Access: | http://dx.doi.org/10.1080/1828051X.2024.2442044 |
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| author | Giulia Rossi Selene Massaro Sheyla Arango Sarah Currò Mauro Spanghero Diana Giannuzzi Stefano Schiavon Lucia Bailoni Franco Tagliapietra |
| author_facet | Giulia Rossi Selene Massaro Sheyla Arango Sarah Currò Mauro Spanghero Diana Giannuzzi Stefano Schiavon Lucia Bailoni Franco Tagliapietra |
| author_sort | Giulia Rossi |
| collection | DOAJ |
| description | This study assessed the impact of rumen ‘fluid not adapted’ (FNA) and of rumen ‘fluid adapted’ (FA) to three additives on in vitro kinetics of gas production and end products of fermentation. The experiment was performed according to a Latin Square Design of 4 cows and 4 experimental periods. The dry cows received a total mixed ration designed for lactating cows without (control) or with 1 g/d of one of three additives (allyl-sulphide, cinnamaldehyde, limonene) that were selected for their known effects on rumen fermentation. The collected rumen fluids (FNA and FA, respectively) were used as inoculum of 4 consecutive in vitro incubations (1 for each experimental period) adding or not adding 30 mg of one of the three pure compounds. The incubations were performed using a commercial equipment to evaluate the kinetics of gas production and collect the end products of fermentation. The results indicated that FA did not influence any fermentation parameters compared to FNA. However, when allyl-sulphide was added in vitro, the effects of this compound tended to be more pronounced with FA than with FNA. This experiment highlights that the three tested pure additives, which show activity on in vitro fermentations, can alter the in vitro activity of rumen fluid collected from cows fed with these compounds. Therefore, the administration of pure additives directly to the cows can influence the rumen microbial activity and the response of in vitro experiments. |
| format | Article |
| id | doaj-art-0263dde503b24f77ad6e62fe3d7ca203 |
| institution | Kabale University |
| issn | 1594-4077 1828-051X |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Italian Journal of Animal Science |
| spelling | doaj-art-0263dde503b24f77ad6e62fe3d7ca2032025-01-02T11:34:22ZengTaylor & Francis GroupItalian Journal of Animal Science1594-40771828-051X2025-12-01241536010.1080/1828051X.2024.24420442442044Rumen fluid from donor cows fed different additives can affect the in vitro fermentation parametersGiulia Rossi0Selene Massaro1Sheyla Arango2Sarah Currò3Mauro Spanghero4Diana Giannuzzi5Stefano Schiavon6Lucia Bailoni7Franco Tagliapietra8Dipartimento di Agronomia, Alimenti, Risorse naturali, Animali e Ambiente (DAFNAE), University of PadovaDipartimento di Agronomia, Alimenti, Risorse naturali, Animali e Ambiente (DAFNAE), University of PadovaDipartimento di Biomedicina Comparata e Alimentazione (BCA), University of PadovaDipartimento di Biomedicina Comparata e Alimentazione (BCA), University of PadovaDipartimento di Scienze Agroalimentari, Ambientali e Animali, University of UdineDipartimento di Agronomia, Alimenti, Risorse naturali, Animali e Ambiente (DAFNAE), University of PadovaDipartimento di Agronomia, Alimenti, Risorse naturali, Animali e Ambiente (DAFNAE), University of PadovaDipartimento di Biomedicina Comparata e Alimentazione (BCA), University of PadovaDipartimento di Agronomia, Alimenti, Risorse naturali, Animali e Ambiente (DAFNAE), University of PadovaThis study assessed the impact of rumen ‘fluid not adapted’ (FNA) and of rumen ‘fluid adapted’ (FA) to three additives on in vitro kinetics of gas production and end products of fermentation. The experiment was performed according to a Latin Square Design of 4 cows and 4 experimental periods. The dry cows received a total mixed ration designed for lactating cows without (control) or with 1 g/d of one of three additives (allyl-sulphide, cinnamaldehyde, limonene) that were selected for their known effects on rumen fermentation. The collected rumen fluids (FNA and FA, respectively) were used as inoculum of 4 consecutive in vitro incubations (1 for each experimental period) adding or not adding 30 mg of one of the three pure compounds. The incubations were performed using a commercial equipment to evaluate the kinetics of gas production and collect the end products of fermentation. The results indicated that FA did not influence any fermentation parameters compared to FNA. However, when allyl-sulphide was added in vitro, the effects of this compound tended to be more pronounced with FA than with FNA. This experiment highlights that the three tested pure additives, which show activity on in vitro fermentations, can alter the in vitro activity of rumen fluid collected from cows fed with these compounds. Therefore, the administration of pure additives directly to the cows can influence the rumen microbial activity and the response of in vitro experiments.http://dx.doi.org/10.1080/1828051X.2024.2442044pure extractgas production kineticmethane productionrumen fluid |
| spellingShingle | Giulia Rossi Selene Massaro Sheyla Arango Sarah Currò Mauro Spanghero Diana Giannuzzi Stefano Schiavon Lucia Bailoni Franco Tagliapietra Rumen fluid from donor cows fed different additives can affect the in vitro fermentation parameters Italian Journal of Animal Science pure extract gas production kinetic methane production rumen fluid |
| title | Rumen fluid from donor cows fed different additives can affect the in vitro fermentation parameters |
| title_full | Rumen fluid from donor cows fed different additives can affect the in vitro fermentation parameters |
| title_fullStr | Rumen fluid from donor cows fed different additives can affect the in vitro fermentation parameters |
| title_full_unstemmed | Rumen fluid from donor cows fed different additives can affect the in vitro fermentation parameters |
| title_short | Rumen fluid from donor cows fed different additives can affect the in vitro fermentation parameters |
| title_sort | rumen fluid from donor cows fed different additives can affect the in vitro fermentation parameters |
| topic | pure extract gas production kinetic methane production rumen fluid |
| url | http://dx.doi.org/10.1080/1828051X.2024.2442044 |
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