Application of RFLP-PCR-Based Identification for Sand Fly Surveillance in an Area Endemic for Kala-Azar in Mymensingh, Bangladesh
Mymensingh is the most endemic district for kala-azar in Bangladesh. Phlebotomus argentipes remains the only known vector although a number of sand fly species are prevalent in this area. Genotyping of sand flies distributed in a VL endemic area was developed by a PCR and restriction-fragment-length...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Journal of Parasitology Research |
| Online Access: | http://dx.doi.org/10.1155/2012/467821 |
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| author | Mohammad Shafiul Alam Hirotomo Kato Mizuho Fukushige Yukiko Wagatsuma Makoto Itoh |
| author_facet | Mohammad Shafiul Alam Hirotomo Kato Mizuho Fukushige Yukiko Wagatsuma Makoto Itoh |
| author_sort | Mohammad Shafiul Alam |
| collection | DOAJ |
| description | Mymensingh is the most endemic district for kala-azar in Bangladesh. Phlebotomus argentipes remains the only known vector although a number of sand fly species are prevalent in this area. Genotyping of sand flies distributed in a VL endemic area was developed by a PCR and restriction-fragment-length polymorphism (RFLP) of 18S rRNA gene of sand fly species. Using the RFLP-PCR analysis with AfaI and HinfI restriction enzymes, P. argentipes, P. papatasi, and Sergentomyia species could be identified. Among 1,055 female sand flies successfully analyzed for the species identification individually, 64.4% flies was classified as Sergentomyia species, whereas 35.6% was identified as P. argentipes and no P. papatasi was found. Although infection of Leishmania within the sand flies was individually examined targeting leishmanial minicircle DNA, none of the 1,055 sand flies examined were positive for Leishmania infection. The RFLP-PCR could be useful tools for taxonomic identification and Leishmania infection monitoring in endemic areas of Bangladesh. |
| format | Article |
| id | doaj-art-024abbc7faba4d5984814c6bce36f99f |
| institution | Kabale University |
| issn | 2090-0023 2090-0031 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Parasitology Research |
| spelling | doaj-art-024abbc7faba4d5984814c6bce36f99f2025-08-20T03:55:32ZengWileyJournal of Parasitology Research2090-00232090-00312012-01-01201210.1155/2012/467821467821Application of RFLP-PCR-Based Identification for Sand Fly Surveillance in an Area Endemic for Kala-Azar in Mymensingh, BangladeshMohammad Shafiul Alam0Hirotomo Kato1Mizuho Fukushige2Yukiko Wagatsuma3Makoto Itoh4Parasitology Laboratory, International Center for Diarrhoeal Disease Research, Bangladesh (ICDDR,B), 68 Shaheed Tajuddin Ahmed Sarani, Mohakhali, Dhaka 1212, BangladeshLaboratory of Parasitology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, JapanDepartment of Epidemiology, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki, Tsukuba 305-8577, JapanDepartment of Epidemiology, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki, Tsukuba 305-8577, JapanDepartment of Parasitology, Aichi Medical University School of Medicine, Nagakute 480-1195, JapanMymensingh is the most endemic district for kala-azar in Bangladesh. Phlebotomus argentipes remains the only known vector although a number of sand fly species are prevalent in this area. Genotyping of sand flies distributed in a VL endemic area was developed by a PCR and restriction-fragment-length polymorphism (RFLP) of 18S rRNA gene of sand fly species. Using the RFLP-PCR analysis with AfaI and HinfI restriction enzymes, P. argentipes, P. papatasi, and Sergentomyia species could be identified. Among 1,055 female sand flies successfully analyzed for the species identification individually, 64.4% flies was classified as Sergentomyia species, whereas 35.6% was identified as P. argentipes and no P. papatasi was found. Although infection of Leishmania within the sand flies was individually examined targeting leishmanial minicircle DNA, none of the 1,055 sand flies examined were positive for Leishmania infection. The RFLP-PCR could be useful tools for taxonomic identification and Leishmania infection monitoring in endemic areas of Bangladesh.http://dx.doi.org/10.1155/2012/467821 |
| spellingShingle | Mohammad Shafiul Alam Hirotomo Kato Mizuho Fukushige Yukiko Wagatsuma Makoto Itoh Application of RFLP-PCR-Based Identification for Sand Fly Surveillance in an Area Endemic for Kala-Azar in Mymensingh, Bangladesh Journal of Parasitology Research |
| title | Application of RFLP-PCR-Based Identification for Sand Fly Surveillance in an Area Endemic for Kala-Azar in Mymensingh, Bangladesh |
| title_full | Application of RFLP-PCR-Based Identification for Sand Fly Surveillance in an Area Endemic for Kala-Azar in Mymensingh, Bangladesh |
| title_fullStr | Application of RFLP-PCR-Based Identification for Sand Fly Surveillance in an Area Endemic for Kala-Azar in Mymensingh, Bangladesh |
| title_full_unstemmed | Application of RFLP-PCR-Based Identification for Sand Fly Surveillance in an Area Endemic for Kala-Azar in Mymensingh, Bangladesh |
| title_short | Application of RFLP-PCR-Based Identification for Sand Fly Surveillance in an Area Endemic for Kala-Azar in Mymensingh, Bangladesh |
| title_sort | application of rflp pcr based identification for sand fly surveillance in an area endemic for kala azar in mymensingh bangladesh |
| url | http://dx.doi.org/10.1155/2012/467821 |
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