Bacterial contamination of sterile angiographic work environments during animal studies.
Bacterial contamination of angiographic materials and fluids has been shown to occur during human angiographic procedures. Angiographic examinations and experiments must be performed under sterile conditions to avoid complications due to contamination and possible subsequent infections. However, dat...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2024-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0311112 |
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| author | Christiane Franz Lara Bender Thorsten Sichtermann Jan Minkenberg Andrea Stockero Christoph Dorn Farzaneh Yousefi Dimah Hasan Manuela Schmiech Rebecca May Sophia Honecker Sebastian Lemmen Omid Nikoubashman Martin Wiesmann Hani Ridwan |
| author_facet | Christiane Franz Lara Bender Thorsten Sichtermann Jan Minkenberg Andrea Stockero Christoph Dorn Farzaneh Yousefi Dimah Hasan Manuela Schmiech Rebecca May Sophia Honecker Sebastian Lemmen Omid Nikoubashman Martin Wiesmann Hani Ridwan |
| author_sort | Christiane Franz |
| collection | DOAJ |
| description | Bacterial contamination of angiographic materials and fluids has been shown to occur during human angiographic procedures. Angiographic examinations and experiments must be performed under sterile conditions to avoid complications due to contamination and possible subsequent infections. However, data regarding the frequency and the clinical consequences are limited. Our aim was to investigate the incidence of bacterial contamination during experimental angiographies. We tested angiographic fluids, syringes and endovascular materials from the angiographic supply tables for bacterial contamination, collecting 252 samples during 18 experimental angiographies in pigs. After sterile filtration, samples were cultured on media, and individual colony-forming units were identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry. Contamination was detected in the majority of samples (60%). There was no angiography in which all samples remained sterile. The highest contamination rates (94%) were found in fluids from the working bowls and on the outer surface of syringes (85%) at the end of angiography. At this time, working bowls were significantly more frequently and extensively contaminated than the control bowls. Among the samples, the frequency and extent of contamination increased with the duration of the experimental angiographic procedures. Our findings show that bacterial contamination during angiography is common and the manipulation of endovascular working materials as well as the duration of angiographic procedures both increase bacterial contamination. While the clinical impact on the laboratory animal remains unclear, the quality of biomedical research mandates that efforts to minimize bacterial contamination should be taken as far as possible. |
| format | Article |
| id | doaj-art-024044dde8e445a3b7e485d6e5fe1c77 |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-024044dde8e445a3b7e485d6e5fe1c772024-11-25T05:31:17ZengPublic Library of Science (PLoS)PLoS ONE1932-62032024-01-011911e031111210.1371/journal.pone.0311112Bacterial contamination of sterile angiographic work environments during animal studies.Christiane FranzLara BenderThorsten SichtermannJan MinkenbergAndrea StockeroChristoph DornFarzaneh YousefiDimah HasanManuela SchmiechRebecca MaySophia HoneckerSebastian LemmenOmid NikoubashmanMartin WiesmannHani RidwanBacterial contamination of angiographic materials and fluids has been shown to occur during human angiographic procedures. Angiographic examinations and experiments must be performed under sterile conditions to avoid complications due to contamination and possible subsequent infections. However, data regarding the frequency and the clinical consequences are limited. Our aim was to investigate the incidence of bacterial contamination during experimental angiographies. We tested angiographic fluids, syringes and endovascular materials from the angiographic supply tables for bacterial contamination, collecting 252 samples during 18 experimental angiographies in pigs. After sterile filtration, samples were cultured on media, and individual colony-forming units were identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry. Contamination was detected in the majority of samples (60%). There was no angiography in which all samples remained sterile. The highest contamination rates (94%) were found in fluids from the working bowls and on the outer surface of syringes (85%) at the end of angiography. At this time, working bowls were significantly more frequently and extensively contaminated than the control bowls. Among the samples, the frequency and extent of contamination increased with the duration of the experimental angiographic procedures. Our findings show that bacterial contamination during angiography is common and the manipulation of endovascular working materials as well as the duration of angiographic procedures both increase bacterial contamination. While the clinical impact on the laboratory animal remains unclear, the quality of biomedical research mandates that efforts to minimize bacterial contamination should be taken as far as possible.https://doi.org/10.1371/journal.pone.0311112 |
| spellingShingle | Christiane Franz Lara Bender Thorsten Sichtermann Jan Minkenberg Andrea Stockero Christoph Dorn Farzaneh Yousefi Dimah Hasan Manuela Schmiech Rebecca May Sophia Honecker Sebastian Lemmen Omid Nikoubashman Martin Wiesmann Hani Ridwan Bacterial contamination of sterile angiographic work environments during animal studies. PLoS ONE |
| title | Bacterial contamination of sterile angiographic work environments during animal studies. |
| title_full | Bacterial contamination of sterile angiographic work environments during animal studies. |
| title_fullStr | Bacterial contamination of sterile angiographic work environments during animal studies. |
| title_full_unstemmed | Bacterial contamination of sterile angiographic work environments during animal studies. |
| title_short | Bacterial contamination of sterile angiographic work environments during animal studies. |
| title_sort | bacterial contamination of sterile angiographic work environments during animal studies |
| url | https://doi.org/10.1371/journal.pone.0311112 |
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