<i>Cannabis sativa</i> L. Extract Increases COX-1, COX-2 and TNF-α in the Hippocampus of Rats with Neuropathic Pain
Inflammation is the critical component of neuropathic pain; therefore, this study aimed to assess the potential anti-inflammatory effects of <i>Cannabis sativa</i> L. extracts in a vincristine-induced model of neuropathic pain. The effects of different doses (5.0–40.0 mg/kg) of two <i...
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2025-01-01
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| author | Joanna Bartkowiak-Wieczorek Małgorzata Jamka Radosław Kujawski Marcin Hołysz Agnieszka Bienert Kamila Czora-Poczwardowska Michał Szulc Przemysław Mikołajczak Anna Bogacz Anna-Maria Wizner Karolina Wielgus Ryszard Słomski Edyta Mądry |
| author_facet | Joanna Bartkowiak-Wieczorek Małgorzata Jamka Radosław Kujawski Marcin Hołysz Agnieszka Bienert Kamila Czora-Poczwardowska Michał Szulc Przemysław Mikołajczak Anna Bogacz Anna-Maria Wizner Karolina Wielgus Ryszard Słomski Edyta Mądry |
| author_sort | Joanna Bartkowiak-Wieczorek |
| collection | DOAJ |
| description | Inflammation is the critical component of neuropathic pain; therefore, this study aimed to assess the potential anti-inflammatory effects of <i>Cannabis sativa</i> L. extracts in a vincristine-induced model of neuropathic pain. The effects of different doses (5.0–40.0 mg/kg) of two <i>Cannabis sativa</i> L. extracts (B and D) on COX-1, COX-2, TNF-α, and NF-κB mRNA and protein levels were examined in the rat hippocampus, cerebral cortex, and blood lymphocytes. There were statistically significant differences in COX-1, COX-2, and TNF-α mRNA and protein expression in the hippocampus and cerebral cortex, with significant differences in COX-2 and TNF-α in the lymphocytes. Extract D dose-dependently increased COX-1 mRNA and protein in the hippocampus and cortex. In contrast, Extract B dose-dependently increased COX-1 mRNA and decreased COX-2 mRNA (in a dose of 7.5 mg/kg) and TNF-α protein levels in the cortex. <i>Cannabis sativa</i> L. extracts significantly influenced the expression of inflammatory genes and proteins, with effects varying based on dose and tissue type. The increased expression of COX-1, COX-2, and TNF-α (in comparison to groups receiving NaCl, vincristine, and gabapentin) in the rat hippocampus and COX-1 in the cerebral cortex suggests that <i>Cannabis</i> may have a pro-inflammatory effect. Due to species specificity, the results of our research based on rats require confirmation in humans. However, <i>Cannabis sativa</i> should be recommended with caution for treating pain with an inflammatory component. |
| format | Article |
| id | doaj-art-01c8fd7933074065a0564f69b3561080 |
| institution | Kabale University |
| issn | 1420-3049 |
| language | English |
| publishDate | 2025-01-01 |
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| series | Molecules |
| spelling | doaj-art-01c8fd7933074065a0564f69b35610802025-01-10T13:19:11ZengMDPI AGMolecules1420-30492025-01-0130119410.3390/molecules30010194<i>Cannabis sativa</i> L. Extract Increases COX-1, COX-2 and TNF-α in the Hippocampus of Rats with Neuropathic PainJoanna Bartkowiak-Wieczorek0Małgorzata Jamka1Radosław Kujawski2Marcin Hołysz3Agnieszka Bienert4Kamila Czora-Poczwardowska5Michał Szulc6Przemysław Mikołajczak7Anna Bogacz8Anna-Maria Wizner9Karolina Wielgus10Ryszard Słomski11Edyta Mądry12Physiology Department, Poznan University of Medical Sciences, 6, Święcickiego Street, 60-781 Poznan, PolandDepartment of Paediatric Gastroenterology and Metabolic Diseases, Poznan University of Medical Sciences, Szpitalna Street 27/33, 60-572 Poznan, PolandDepartment of Pharmacology, Poznan University of Medical Sciences, 3, Rokietnicka Street, 60-806 Poznan, PolandDepartment of Biochemistry and Molecular Biology, Poznan University of Medical Sciences, 6, Swiecickiego Steet, 60-781 Poznan, PolandDepartment of Pharmacology, Poznan University of Medical Sciences, 3, Rokietnicka Street, 60-806 Poznan, PolandDepartment of Pharmacology, Poznan University of Medical Sciences, 3, Rokietnicka Street, 60-806 Poznan, PolandDepartment of Pharmacology, Poznan University of Medical Sciences, 3, Rokietnicka Street, 60-806 Poznan, PolandDepartment of Pharmacology, Poznan University of Medical Sciences, 3, Rokietnicka Street, 60-806 Poznan, PolandPhysiology Department, Poznan University of Medical Sciences, 6, Święcickiego Street, 60-781 Poznan, PolandDepartment of Clinical Pharmacy and Biopharmacy, Poznan University of Medical Sciences, 3, Rokietnicka Street, 60-806 Poznan, PolandDepartment of Paediatric Gastroenterology and Metabolic Diseases, Poznan University of Medical Sciences, Szpitalna Street 27/33, 60-572 Poznan, PolandDepartment of Biotechnology, Institute of Natural Fibres and Medicinal Plants—National Research Institute, Wojska Polskiego 71B, 60-630 Poznan, PolandPhysiology Department, Poznan University of Medical Sciences, 6, Święcickiego Street, 60-781 Poznan, PolandInflammation is the critical component of neuropathic pain; therefore, this study aimed to assess the potential anti-inflammatory effects of <i>Cannabis sativa</i> L. extracts in a vincristine-induced model of neuropathic pain. The effects of different doses (5.0–40.0 mg/kg) of two <i>Cannabis sativa</i> L. extracts (B and D) on COX-1, COX-2, TNF-α, and NF-κB mRNA and protein levels were examined in the rat hippocampus, cerebral cortex, and blood lymphocytes. There were statistically significant differences in COX-1, COX-2, and TNF-α mRNA and protein expression in the hippocampus and cerebral cortex, with significant differences in COX-2 and TNF-α in the lymphocytes. Extract D dose-dependently increased COX-1 mRNA and protein in the hippocampus and cortex. In contrast, Extract B dose-dependently increased COX-1 mRNA and decreased COX-2 mRNA (in a dose of 7.5 mg/kg) and TNF-α protein levels in the cortex. <i>Cannabis sativa</i> L. extracts significantly influenced the expression of inflammatory genes and proteins, with effects varying based on dose and tissue type. The increased expression of COX-1, COX-2, and TNF-α (in comparison to groups receiving NaCl, vincristine, and gabapentin) in the rat hippocampus and COX-1 in the cerebral cortex suggests that <i>Cannabis</i> may have a pro-inflammatory effect. Due to species specificity, the results of our research based on rats require confirmation in humans. However, <i>Cannabis sativa</i> should be recommended with caution for treating pain with an inflammatory component.https://www.mdpi.com/1420-3049/30/1/194CBDTHCvincristinelymphocytescerebral cortexNFκB |
| spellingShingle | Joanna Bartkowiak-Wieczorek Małgorzata Jamka Radosław Kujawski Marcin Hołysz Agnieszka Bienert Kamila Czora-Poczwardowska Michał Szulc Przemysław Mikołajczak Anna Bogacz Anna-Maria Wizner Karolina Wielgus Ryszard Słomski Edyta Mądry <i>Cannabis sativa</i> L. Extract Increases COX-1, COX-2 and TNF-α in the Hippocampus of Rats with Neuropathic Pain Molecules CBD THC vincristine lymphocytes cerebral cortex NFκB |
| title | <i>Cannabis sativa</i> L. Extract Increases COX-1, COX-2 and TNF-α in the Hippocampus of Rats with Neuropathic Pain |
| title_full | <i>Cannabis sativa</i> L. Extract Increases COX-1, COX-2 and TNF-α in the Hippocampus of Rats with Neuropathic Pain |
| title_fullStr | <i>Cannabis sativa</i> L. Extract Increases COX-1, COX-2 and TNF-α in the Hippocampus of Rats with Neuropathic Pain |
| title_full_unstemmed | <i>Cannabis sativa</i> L. Extract Increases COX-1, COX-2 and TNF-α in the Hippocampus of Rats with Neuropathic Pain |
| title_short | <i>Cannabis sativa</i> L. Extract Increases COX-1, COX-2 and TNF-α in the Hippocampus of Rats with Neuropathic Pain |
| title_sort | i cannabis sativa i l extract increases cox 1 cox 2 and tnf α in the hippocampus of rats with neuropathic pain |
| topic | CBD THC vincristine lymphocytes cerebral cortex NFκB |
| url | https://www.mdpi.com/1420-3049/30/1/194 |
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