Generation of an induced pluripotent stem cell line from a type 1 neurofibromatosis patient with NF1 mutation
A human induced pluripotent stem cell (iPSC) line was generated from patient with type 1 neurofibromatosis (NF-1), carrying heterozygous mutation in NF1 gene. Peripheral blood mononuclear cells (PBMCs) were reprogrammed using non-integrating delivery of KFL4, OCT4, SOX2, BCL-XL and c-MYC. The iPSC l...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-12-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506124002666 |
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| author | Hongmei Xin Yixiao Li Kaihui Zhang Aihua Ji Meili Fan |
| author_facet | Hongmei Xin Yixiao Li Kaihui Zhang Aihua Ji Meili Fan |
| author_sort | Hongmei Xin |
| collection | DOAJ |
| description | A human induced pluripotent stem cell (iPSC) line was generated from patient with type 1 neurofibromatosis (NF-1), carrying heterozygous mutation in NF1 gene. Peripheral blood mononuclear cells (PBMCs) were reprogrammed using non-integrating delivery of KFL4, OCT4, SOX2, BCL-XL and c-MYC. The iPSC line expresses pluripotency markers, displays a normal karyotype, and is able to differentiate into three germ layers in vitro. This iPSC line represents a valuable cell model for NF1 in humans. |
| format | Article |
| id | doaj-art-013e47ee313d4fd2b40ec1c3e3ea1a3c |
| institution | Kabale University |
| issn | 1873-5061 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj-art-013e47ee313d4fd2b40ec1c3e3ea1a3c2024-12-13T10:56:50ZengElsevierStem Cell Research1873-50612024-12-0181103568Generation of an induced pluripotent stem cell line from a type 1 neurofibromatosis patient with NF1 mutationHongmei Xin0Yixiao Li1Kaihui Zhang2Aihua Ji3Meili Fan4Pediatric Research Institute, Children’s Hospital Affiliated to Shandong University, Jinan 250022, ChinaPediatric Research Institute, Children’s Hospital Affiliated to Shandong University, Jinan 250022, China; Department of Ophthalmology, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250001, ChinaPediatric Research Institute, Children’s Hospital Affiliated to Shandong University, Jinan 250022, ChinaDepartment of Dermatology, Children’s Hospital Affiliated to Shandong University (Jinan Children’s Hospital), Jinan, Shandong 250022, China; Corresponding authors.Tuina Department, Children’s Hospital Affiliated to Shandong University (Jinan Children’s Hospital), Jinan, Shandong 250022, China; Corresponding authors.A human induced pluripotent stem cell (iPSC) line was generated from patient with type 1 neurofibromatosis (NF-1), carrying heterozygous mutation in NF1 gene. Peripheral blood mononuclear cells (PBMCs) were reprogrammed using non-integrating delivery of KFL4, OCT4, SOX2, BCL-XL and c-MYC. The iPSC line expresses pluripotency markers, displays a normal karyotype, and is able to differentiate into three germ layers in vitro. This iPSC line represents a valuable cell model for NF1 in humans.http://www.sciencedirect.com/science/article/pii/S1873506124002666 |
| spellingShingle | Hongmei Xin Yixiao Li Kaihui Zhang Aihua Ji Meili Fan Generation of an induced pluripotent stem cell line from a type 1 neurofibromatosis patient with NF1 mutation Stem Cell Research |
| title | Generation of an induced pluripotent stem cell line from a type 1 neurofibromatosis patient with NF1 mutation |
| title_full | Generation of an induced pluripotent stem cell line from a type 1 neurofibromatosis patient with NF1 mutation |
| title_fullStr | Generation of an induced pluripotent stem cell line from a type 1 neurofibromatosis patient with NF1 mutation |
| title_full_unstemmed | Generation of an induced pluripotent stem cell line from a type 1 neurofibromatosis patient with NF1 mutation |
| title_short | Generation of an induced pluripotent stem cell line from a type 1 neurofibromatosis patient with NF1 mutation |
| title_sort | generation of an induced pluripotent stem cell line from a type 1 neurofibromatosis patient with nf1 mutation |
| url | http://www.sciencedirect.com/science/article/pii/S1873506124002666 |
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