Protocol for the simultaneous isolation of DNA, RNA, and miRNA from a single archived Kaposi sarcoma biopsy

Protocol for the simultaneous isolation of DNA, RNA, and miRNA from a single archived Kaposi sarcoma biopsy

Summary: Kaposi sarcoma (KS) punch biopsies present unique challenges for extracting nucleic acids, which can be exacerbated by their long-term stabilization in RNAlater. Here, we present a protocol for simultaneously isolating DNA, RNA, and miRNA from a single KS punch biopsy. We detail the steps f...

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Bibliographic Details
Main Authors: Larissa L.S. Scholte, Justin Browne, David J. Nolan, Peyton St. John, Katherine Tracy, Rafaela S. Thur, Ghangzhao Li, Susanna L. Lamers, Paige Bracci, Michael S. McGrath, Jeffrey M. Bethony
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:STAR Protocols
Subjects:
Cancer
Sequencing
Molecular Biology
Gene Expression
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724005306
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