Combining 2′-fucosyllactose and galacto-oligosaccharides exerts anti-inflammatory effects and promotes gut health
ABSTRACT: This study investigated the potential of 2′-fucosyllactose (2′-FL) and galacto-oligosaccharides (GOS) combinations as a novel and cost-effective substitute for human milk oligosaccharides (HMO) in promoting gut health and reducing inflammation. In vitro studies using caco-2 cells showed th...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-12-01
|
| Series: | Journal of Dairy Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022030224011172 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846150216379531264 |
|---|---|
| author | Sewon Park Yoonhee Park Yu-Jin Jeong Jun Gu Oh Hee Joon Yoo Jiyeon Yang Jung-il Kwon Kwang-Won Lee |
| author_facet | Sewon Park Yoonhee Park Yu-Jin Jeong Jun Gu Oh Hee Joon Yoo Jiyeon Yang Jung-il Kwon Kwang-Won Lee |
| author_sort | Sewon Park |
| collection | DOAJ |
| description | ABSTRACT: This study investigated the potential of 2′-fucosyllactose (2′-FL) and galacto-oligosaccharides (GOS) combinations as a novel and cost-effective substitute for human milk oligosaccharides (HMO) in promoting gut health and reducing inflammation. In vitro studies using caco-2 cells showed that 2′-FL and GOS combinations (H1 = GOS:2′-FL ratio of 1.8:1; H2 = GOS:2′-FL ratio of 3.6:1) reduced LPS-induced inflammation by decreasing pro-inflammatory markers, whereas individual treatments had no significant effects. In a mouse model of dextran sulfate sodium (DSS)-induced colitis, combined 2′-FL and GOS supplementation alleviated symptoms, improved gut permeability, and enhanced intestinal structure, with the GH1 group (H1 combo with DSS) being the most effective. 2′-Fucosyllactose and GOS combinations also enhanced short-chain fatty acid production in infant fecal batch fermentation and mouse fecal analysis, with GH1 showing the most promising results. The GH1 supplementation altered gut microbiota in mice with DSS-induced colitis, promoting microbial diversity and a more balanced Firmicutes to Bacteroidota ratio. Infant formula products (IFP) containing 2′-FL and GOS combinations (IFP2 = 174 mg of GOS and 95 mg of 2′-FL per 14 g serving, 1.8:1 ratio; IFP3 = 174 mg of GOS and 48 mg of 2′-FL per 14 g serving, 3.6:1 ratio) demonstrated gastrointestinal protective and anti-inflammatory properties in a co-culture model of caco-2 and THP-1 cells. These findings suggest that 2′-FL and GOS combinations have potential applications in advanced infant formulas and supplements to promote gut health and reduce inflammation. |
| format | Article |
| id | doaj-art-00f732b8b813423a9bbe4ba8ee92669d |
| institution | Kabale University |
| issn | 0022-0302 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Dairy Science |
| spelling | doaj-art-00f732b8b813423a9bbe4ba8ee92669d2024-11-29T06:22:28ZengElsevierJournal of Dairy Science0022-03022024-12-01107121020310220Combining 2′-fucosyllactose and galacto-oligosaccharides exerts anti-inflammatory effects and promotes gut healthSewon Park0Yoonhee Park1Yu-Jin Jeong2Jun Gu Oh3Hee Joon Yoo4Jiyeon Yang5Jung-il Kwon6Kwang-Won Lee7Department of Biotechnology, College of Life Sciences and Biotechnology, 02841, Korea University, Seoul, Republic of KoreaDepartment of Biotechnology, College of Life Sciences and Biotechnology, 02841, Korea University, Seoul, Republic of KoreaDepartment of Biotechnology, College of Life Sciences and Biotechnology, 02841, Korea University, Seoul, Republic of KoreaDepartment of Biotechnology, College of Life Sciences and Biotechnology, 02841, Korea University, Seoul, Republic of KoreaDepartment of Biotechnology, College of Life Sciences and Biotechnology, 02841, Korea University, Seoul, Republic of KoreaDepartment of Integrated Biomedical and Life Science, Graduate School, 02841, Korea University, Seoul, Republic of KoreaDepartment of Integrated Biomedical and Life Science, Graduate School, 02841, Korea University, Seoul, Republic of KoreaDepartment of Biotechnology, College of Life Sciences and Biotechnology, 02841, Korea University, Seoul, Republic of Korea; Corresponding authorABSTRACT: This study investigated the potential of 2′-fucosyllactose (2′-FL) and galacto-oligosaccharides (GOS) combinations as a novel and cost-effective substitute for human milk oligosaccharides (HMO) in promoting gut health and reducing inflammation. In vitro studies using caco-2 cells showed that 2′-FL and GOS combinations (H1 = GOS:2′-FL ratio of 1.8:1; H2 = GOS:2′-FL ratio of 3.6:1) reduced LPS-induced inflammation by decreasing pro-inflammatory markers, whereas individual treatments had no significant effects. In a mouse model of dextran sulfate sodium (DSS)-induced colitis, combined 2′-FL and GOS supplementation alleviated symptoms, improved gut permeability, and enhanced intestinal structure, with the GH1 group (H1 combo with DSS) being the most effective. 2′-Fucosyllactose and GOS combinations also enhanced short-chain fatty acid production in infant fecal batch fermentation and mouse fecal analysis, with GH1 showing the most promising results. The GH1 supplementation altered gut microbiota in mice with DSS-induced colitis, promoting microbial diversity and a more balanced Firmicutes to Bacteroidota ratio. Infant formula products (IFP) containing 2′-FL and GOS combinations (IFP2 = 174 mg of GOS and 95 mg of 2′-FL per 14 g serving, 1.8:1 ratio; IFP3 = 174 mg of GOS and 48 mg of 2′-FL per 14 g serving, 3.6:1 ratio) demonstrated gastrointestinal protective and anti-inflammatory properties in a co-culture model of caco-2 and THP-1 cells. These findings suggest that 2′-FL and GOS combinations have potential applications in advanced infant formulas and supplements to promote gut health and reduce inflammation.http://www.sciencedirect.com/science/article/pii/S00220302240111722′-fucosyllactosegut healthinfant formulahuman milk oligosaccharides |
| spellingShingle | Sewon Park Yoonhee Park Yu-Jin Jeong Jun Gu Oh Hee Joon Yoo Jiyeon Yang Jung-il Kwon Kwang-Won Lee Combining 2′-fucosyllactose and galacto-oligosaccharides exerts anti-inflammatory effects and promotes gut health Journal of Dairy Science 2′-fucosyllactose gut health infant formula human milk oligosaccharides |
| title | Combining 2′-fucosyllactose and galacto-oligosaccharides exerts anti-inflammatory effects and promotes gut health |
| title_full | Combining 2′-fucosyllactose and galacto-oligosaccharides exerts anti-inflammatory effects and promotes gut health |
| title_fullStr | Combining 2′-fucosyllactose and galacto-oligosaccharides exerts anti-inflammatory effects and promotes gut health |
| title_full_unstemmed | Combining 2′-fucosyllactose and galacto-oligosaccharides exerts anti-inflammatory effects and promotes gut health |
| title_short | Combining 2′-fucosyllactose and galacto-oligosaccharides exerts anti-inflammatory effects and promotes gut health |
| title_sort | combining 2 fucosyllactose and galacto oligosaccharides exerts anti inflammatory effects and promotes gut health |
| topic | 2′-fucosyllactose gut health infant formula human milk oligosaccharides |
| url | http://www.sciencedirect.com/science/article/pii/S0022030224011172 |
| work_keys_str_mv | AT sewonpark combining2fucosyllactoseandgalactooligosaccharidesexertsantiinflammatoryeffectsandpromotesguthealth AT yoonheepark combining2fucosyllactoseandgalactooligosaccharidesexertsantiinflammatoryeffectsandpromotesguthealth AT yujinjeong combining2fucosyllactoseandgalactooligosaccharidesexertsantiinflammatoryeffectsandpromotesguthealth AT junguoh combining2fucosyllactoseandgalactooligosaccharidesexertsantiinflammatoryeffectsandpromotesguthealth AT heejoonyoo combining2fucosyllactoseandgalactooligosaccharidesexertsantiinflammatoryeffectsandpromotesguthealth AT jiyeonyang combining2fucosyllactoseandgalactooligosaccharidesexertsantiinflammatoryeffectsandpromotesguthealth AT jungilkwon combining2fucosyllactoseandgalactooligosaccharidesexertsantiinflammatoryeffectsandpromotesguthealth AT kwangwonlee combining2fucosyllactoseandgalactooligosaccharidesexertsantiinflammatoryeffectsandpromotesguthealth |