Efficient DNA-Free Protoplast Gene Editing of Elite Winegrape Cultivars for the Generation of Clones With Reduced Downy Mildew Susceptibility
Conclusions: This study has demonstrated that a relatively simple and robust protoplast isolation, DNA-free protoplast transfection and plant regeneration workflow can be used to efficiently produce nontransgenic, diploid, edited clones with desired phenotypes of four elite winegrape cultivars, incl...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2025-01-01
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| Series: | Australian Journal of Grape and Wine Research |
| Online Access: | http://dx.doi.org/10.1155/ajgw/8867814 |
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| _version_ | 1849319219554942976 |
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| author | Christine Böttcher Debra McDavid Angelica M. Jermakow Patricia Iocco-Corena Nayana Arunasiri Suzanne M. Maffei Paul K. Boss |
| author_facet | Christine Böttcher Debra McDavid Angelica M. Jermakow Patricia Iocco-Corena Nayana Arunasiri Suzanne M. Maffei Paul K. Boss |
| author_sort | Christine Böttcher |
| collection | DOAJ |
| description | Conclusions: This study has demonstrated that a relatively simple and robust protoplast isolation, DNA-free protoplast transfection and plant regeneration workflow can be used to efficiently produce nontransgenic, diploid, edited clones with desired phenotypes of four elite winegrape cultivars, including the highly recalcitrant Cabernet Sauvignon. |
| format | Article |
| id | doaj-art-00e8c44f12c240cbb5e195b68b0fcba4 |
| institution | Kabale University |
| issn | 1755-0238 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Australian Journal of Grape and Wine Research |
| spelling | doaj-art-00e8c44f12c240cbb5e195b68b0fcba42025-08-20T03:50:32ZengWileyAustralian Journal of Grape and Wine Research1755-02382025-01-01202510.1155/ajgw/8867814Efficient DNA-Free Protoplast Gene Editing of Elite Winegrape Cultivars for the Generation of Clones With Reduced Downy Mildew SusceptibilityChristine Böttcher0Debra McDavid1Angelica M. Jermakow2Patricia Iocco-Corena3Nayana Arunasiri4Suzanne M. Maffei5Paul K. Boss6Agriculture and FoodAgriculture and FoodAgriculture and FoodAgriculture and FoodAgriculture and FoodAgriculture and FoodAgriculture and FoodConclusions: This study has demonstrated that a relatively simple and robust protoplast isolation, DNA-free protoplast transfection and plant regeneration workflow can be used to efficiently produce nontransgenic, diploid, edited clones with desired phenotypes of four elite winegrape cultivars, including the highly recalcitrant Cabernet Sauvignon.http://dx.doi.org/10.1155/ajgw/8867814 |
| spellingShingle | Christine Böttcher Debra McDavid Angelica M. Jermakow Patricia Iocco-Corena Nayana Arunasiri Suzanne M. Maffei Paul K. Boss Efficient DNA-Free Protoplast Gene Editing of Elite Winegrape Cultivars for the Generation of Clones With Reduced Downy Mildew Susceptibility Australian Journal of Grape and Wine Research |
| title | Efficient DNA-Free Protoplast Gene Editing of Elite Winegrape Cultivars for the Generation of Clones With Reduced Downy Mildew Susceptibility |
| title_full | Efficient DNA-Free Protoplast Gene Editing of Elite Winegrape Cultivars for the Generation of Clones With Reduced Downy Mildew Susceptibility |
| title_fullStr | Efficient DNA-Free Protoplast Gene Editing of Elite Winegrape Cultivars for the Generation of Clones With Reduced Downy Mildew Susceptibility |
| title_full_unstemmed | Efficient DNA-Free Protoplast Gene Editing of Elite Winegrape Cultivars for the Generation of Clones With Reduced Downy Mildew Susceptibility |
| title_short | Efficient DNA-Free Protoplast Gene Editing of Elite Winegrape Cultivars for the Generation of Clones With Reduced Downy Mildew Susceptibility |
| title_sort | efficient dna free protoplast gene editing of elite winegrape cultivars for the generation of clones with reduced downy mildew susceptibility |
| url | http://dx.doi.org/10.1155/ajgw/8867814 |
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