Efficient DNA-Free Protoplast Gene Editing of Elite Winegrape Cultivars for the Generation of Clones With Reduced Downy Mildew Susceptibility

Efficient DNA-Free Protoplast Gene Editing of Elite Winegrape Cultivars for the Generation of Clones With Reduced Downy Mildew Susceptibility

Conclusions: This study has demonstrated that a relatively simple and robust protoplast isolation, DNA-free protoplast transfection and plant regeneration workflow can be used to efficiently produce nontransgenic, diploid, edited clones with desired phenotypes of four elite winegrape cultivars, incl...

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Bibliographic Details
Main Authors: Christine Böttcher, Debra McDavid, Angelica M. Jermakow, Patricia Iocco-Corena, Nayana Arunasiri, Suzanne M. Maffei, Paul K. Boss
Format: Article
Language:English
Published: Wiley 2025-01-01
Series:Australian Journal of Grape and Wine Research
Online Access:http://dx.doi.org/10.1155/ajgw/8867814
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Summary:Conclusions: This study has demonstrated that a relatively simple and robust protoplast isolation, DNA-free protoplast transfection and plant regeneration workflow can be used to efficiently produce nontransgenic, diploid, edited clones with desired phenotypes of four elite winegrape cultivars, including the highly recalcitrant Cabernet Sauvignon.
ISSN:1755-0238

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