An Evaluation of Organic Biostimulants as a Tool for the Sustainable Management of Viral Infections in Zucchini Plants

In agriculture, new and sustainable strategies are increasingly demanded to integrate the traditional management of viral diseases based on the use of virus-free propagation materials and resistant or tolerant cultivars and on the control of insect vectors. Among the possible Integrated Pest Managem...

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Main Authors: Carla Libia Corrado, Livia Donati, Anna Taglienti, Luca Ferretti, Francesco Faggioli, Massimo Reverberi, Sabrina Bertin
Format: Article
Language:English
Published: MDPI AG 2024-11-01
Series:Horticulturae
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Online Access:https://www.mdpi.com/2311-7524/10/11/1176
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author Carla Libia Corrado
Livia Donati
Anna Taglienti
Luca Ferretti
Francesco Faggioli
Massimo Reverberi
Sabrina Bertin
author_facet Carla Libia Corrado
Livia Donati
Anna Taglienti
Luca Ferretti
Francesco Faggioli
Massimo Reverberi
Sabrina Bertin
author_sort Carla Libia Corrado
collection DOAJ
description In agriculture, new and sustainable strategies are increasingly demanded to integrate the traditional management of viral diseases based on the use of virus-free propagation materials and resistant or tolerant cultivars and on the control of insect vectors. Among the possible Integrated Pest Management (IPM) approaches, organic biostimulants have shown promising results in enhancing plant tolerance to virus infections by improving plant fitness and productivity and modulating metabolic functions. In this study, the combination of two organic biostimulants, Alert D-Max and Resil EVO Q, composed of seaweed and alfalfa extracts, enzymatic hydrolysates, and micronized zeolite, was applied on the leaves and roots of zucchini squashes, both healthy and infected by zucchini yellow mosaic virus (ZYMV). Four applications were scheduled based on ZYMV inoculation timing, and plant vegetative and reproductive parameters were recorded along with the virus titre and symptom severity. The modulation of the expression of specific genes potentially involved in pattern-triggered immunity (PTI), systemic acquired resistance (SAR), and oxidative stress defence pathways was also investigated. Besides increasing the general fitness of the healthy plants, the biostimulants significantly improved the production of flowers and fruits of the infected plants, with a potential positive impact on their productivity. The repeated biostimulant applications also led to a one-tenth reduction in ZYMV titre over time and induced a progressive slowdown of symptom severity. Genes associated with SAR and PTI were up-regulated after biostimulant applications, suggesting the biostimulant-based priming of plant defence mechanisms. Due to the observed beneficial effects, the tested biostimulant mix can be an effective component of the IPM of cucurbit crops, acting as a sustainable practice for enhancing plant fitness and tolerance to potyviruses.
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spelling doaj-art-00d67f7a6cdf4671958ced688867bb902024-11-26T18:06:01ZengMDPI AGHorticulturae2311-75242024-11-011011117610.3390/horticulturae10111176An Evaluation of Organic Biostimulants as a Tool for the Sustainable Management of Viral Infections in Zucchini PlantsCarla Libia Corrado0Livia Donati1Anna Taglienti2Luca Ferretti3Francesco Faggioli4Massimo Reverberi5Sabrina Bertin6Department of Environmental Biology, Sapienza University of Rome, Piazzale Aldo Moro, 5, 00185 Rome, ItalyCREA Research Centre for Plant Protection and Certification, Via C.G. Bertero 22, 00156 Rome, ItalyCREA Research Centre for Plant Protection and Certification, Via C.G. Bertero 22, 00156 Rome, ItalyCREA Research Centre for Plant Protection and Certification, Via C.G. Bertero 22, 00156 Rome, ItalyCREA Research Centre for Plant Protection and Certification, Via C.G. Bertero 22, 00156 Rome, ItalyDepartment of Environmental Biology, Sapienza University of Rome, Piazzale Aldo Moro, 5, 00185 Rome, ItalyCREA Research Centre for Plant Protection and Certification, Via C.G. Bertero 22, 00156 Rome, ItalyIn agriculture, new and sustainable strategies are increasingly demanded to integrate the traditional management of viral diseases based on the use of virus-free propagation materials and resistant or tolerant cultivars and on the control of insect vectors. Among the possible Integrated Pest Management (IPM) approaches, organic biostimulants have shown promising results in enhancing plant tolerance to virus infections by improving plant fitness and productivity and modulating metabolic functions. In this study, the combination of two organic biostimulants, Alert D-Max and Resil EVO Q, composed of seaweed and alfalfa extracts, enzymatic hydrolysates, and micronized zeolite, was applied on the leaves and roots of zucchini squashes, both healthy and infected by zucchini yellow mosaic virus (ZYMV). Four applications were scheduled based on ZYMV inoculation timing, and plant vegetative and reproductive parameters were recorded along with the virus titre and symptom severity. The modulation of the expression of specific genes potentially involved in pattern-triggered immunity (PTI), systemic acquired resistance (SAR), and oxidative stress defence pathways was also investigated. Besides increasing the general fitness of the healthy plants, the biostimulants significantly improved the production of flowers and fruits of the infected plants, with a potential positive impact on their productivity. The repeated biostimulant applications also led to a one-tenth reduction in ZYMV titre over time and induced a progressive slowdown of symptom severity. Genes associated with SAR and PTI were up-regulated after biostimulant applications, suggesting the biostimulant-based priming of plant defence mechanisms. Due to the observed beneficial effects, the tested biostimulant mix can be an effective component of the IPM of cucurbit crops, acting as a sustainable practice for enhancing plant fitness and tolerance to potyviruses.https://www.mdpi.com/2311-7524/10/11/1176zucchini yellow mosaic viruspotyvirusCucurbitaceaeplant fitnessplant defence pathways
spellingShingle Carla Libia Corrado
Livia Donati
Anna Taglienti
Luca Ferretti
Francesco Faggioli
Massimo Reverberi
Sabrina Bertin
An Evaluation of Organic Biostimulants as a Tool for the Sustainable Management of Viral Infections in Zucchini Plants
Horticulturae
zucchini yellow mosaic virus
potyvirus
Cucurbitaceae
plant fitness
plant defence pathways
title An Evaluation of Organic Biostimulants as a Tool for the Sustainable Management of Viral Infections in Zucchini Plants
title_full An Evaluation of Organic Biostimulants as a Tool for the Sustainable Management of Viral Infections in Zucchini Plants
title_fullStr An Evaluation of Organic Biostimulants as a Tool for the Sustainable Management of Viral Infections in Zucchini Plants
title_full_unstemmed An Evaluation of Organic Biostimulants as a Tool for the Sustainable Management of Viral Infections in Zucchini Plants
title_short An Evaluation of Organic Biostimulants as a Tool for the Sustainable Management of Viral Infections in Zucchini Plants
title_sort evaluation of organic biostimulants as a tool for the sustainable management of viral infections in zucchini plants
topic zucchini yellow mosaic virus
potyvirus
Cucurbitaceae
plant fitness
plant defence pathways
url https://www.mdpi.com/2311-7524/10/11/1176
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