Effect of lipoic acid on spermatological and biochemical parameters following equilibration and freeze-thawing of ram semen
<b>Aim:</b> The aim of this study was to investigate the effects of different doses of lipoic acid added into dilution extender, following equilibration and freeze-thawing of ram semen on spermatologic and biochemical parameters.<p> <b>Materials and Methods:</b> The eja...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Selcuk University Press
|
| Series: | Eurasian Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=572 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841561457795268608 |
|---|---|
| author | Nuri Başpınar Kenan Çoyan Mustafa Numan Bucak Ali Doğan Ömür Mehmet Bozkurt Ataman Pınar Peker Akalın Şükrü Güngör Caner Öztürk |
| author_facet | Nuri Başpınar Kenan Çoyan Mustafa Numan Bucak Ali Doğan Ömür Mehmet Bozkurt Ataman Pınar Peker Akalın Şükrü Güngör Caner Öztürk |
| author_sort | Nuri Başpınar |
| collection | DOAJ |
| description | <b>Aim:</b> The aim of this study was to investigate the effects of different doses of lipoic acid added into dilution extender, following equilibration and freeze-thawing of ram semen on spermatologic and biochemical parameters.<p>
<b>Materials and Methods:</b> The ejaculates collected from 6 mature Konya Merino rams were used in the research. Ejaculates containing approximately 400x106 spermatozoa were diluted at 37 ºC with the Tris-based extender containing lipoic acid at the doses of 1, 2, 4 mM and no additive (control), and then, they were frozen.<p>
<b>Results:</b> After equilibration, all doses of lipoic acid did not affect the sperm parameters whereas lipoic acid 1 mM (74.3%) ameliorated motility compared to both control (62.5%) and lipoic acid 4 mM (65.0%) (p<0.001) groups after freeze-thawing. Lipoic acid did not affect HOS test and acrosome integrity of spermatozoa following freeze-thaw-ing. Also, the supplementation of lipoic acid at 1, 2 and 4 mM doses did not provide any significant effect on the levels ofbiochemical parameters (p>0.05).<p>
<b>Conclusion:</b> Addition of 1 mM lipoic acid to ram semen increases the motility after freeze-thawing process. However, dose treatments of lipoic acid in different extenders and animal species-breeds are required to be performed. |
| format | Article |
| id | doaj-art-00b78c7dd0224a64a638a0e4afade2bf |
| institution | Kabale University |
| issn | 1309-6958 2146-1953 |
| language | English |
| publisher | Selcuk University Press |
| record_format | Article |
| series | Eurasian Journal of Veterinary Sciences |
| spelling | doaj-art-00b78c7dd0224a64a638a0e4afade2bf2025-01-03T01:36:14ZengSelcuk University PressEurasian Journal of Veterinary Sciences1309-69582146-19532728792572Effect of lipoic acid on spermatological and biochemical parameters following equilibration and freeze-thawing of ram semenNuri Başpınar0Kenan Çoyan1Mustafa Numan Bucak2Ali Doğan Ömür3Mehmet Bozkurt Ataman4Pınar Peker Akalın5Şükrü Güngör6Caner Öztürk7Selçuk Üniversitesi, Veteriner Fakültesi, Biyokimya Anabilim Dalı, Kampüs, Konya, TürkiyeSelçuk Üniversitesi, Veteriner Fakültesi, Dölerme ve Suni Tohumlama Anabilim Dalı, Kampüs, Konya, TürkiyeSelçuk Üniversitesi, Veteriner Fakültesi, Dölerme ve Suni Tohumlama Anabilim Dalı, Kampüs, Konya, TürkiyeSelçuk Üniversitesi, Veteriner Fakültesi, Dölerme ve Suni Tohumlama Anabilim Dalı, Kampüs, Konya, TürkiyeSelçuk Üniversitesi, Veteriner Fakültesi, Dölerme ve Suni Tohumlama Anabilim Dalı, Kampüs, Konya, TürkiyeTarım ve Köyişleri Bakanlığı, Merkez Veteriner Kontrol ve Araştırma Enstitüsü, Ankara, TürkiyeSelçuk Üniversitesi, Veteriner Fakültesi, Dölerme ve Suni Tohumlama Anabilim Dalı, Kampüs, Konya, TürkiyeSelçuk Üniversitesi, Veteriner Fakültesi, Dölerme ve Suni Tohumlama Anabilim Dalı, Kampüs, Konya, Türkiye<b>Aim:</b> The aim of this study was to investigate the effects of different doses of lipoic acid added into dilution extender, following equilibration and freeze-thawing of ram semen on spermatologic and biochemical parameters.<p> <b>Materials and Methods:</b> The ejaculates collected from 6 mature Konya Merino rams were used in the research. Ejaculates containing approximately 400x106 spermatozoa were diluted at 37 ºC with the Tris-based extender containing lipoic acid at the doses of 1, 2, 4 mM and no additive (control), and then, they were frozen.<p> <b>Results:</b> After equilibration, all doses of lipoic acid did not affect the sperm parameters whereas lipoic acid 1 mM (74.3%) ameliorated motility compared to both control (62.5%) and lipoic acid 4 mM (65.0%) (p<0.001) groups after freeze-thawing. Lipoic acid did not affect HOS test and acrosome integrity of spermatozoa following freeze-thaw-ing. Also, the supplementation of lipoic acid at 1, 2 and 4 mM doses did not provide any significant effect on the levels ofbiochemical parameters (p>0.05).<p> <b>Conclusion:</b> Addition of 1 mM lipoic acid to ram semen increases the motility after freeze-thawing process. However, dose treatments of lipoic acid in different extenders and animal species-breeds are required to be performed.http://eurasianjvetsci.org/pdf.php3?id=572ram semenfreezing-thawinglipoic acid |
| spellingShingle | Nuri Başpınar Kenan Çoyan Mustafa Numan Bucak Ali Doğan Ömür Mehmet Bozkurt Ataman Pınar Peker Akalın Şükrü Güngör Caner Öztürk Effect of lipoic acid on spermatological and biochemical parameters following equilibration and freeze-thawing of ram semen Eurasian Journal of Veterinary Sciences ram semen freezing-thawing lipoic acid |
| title | Effect of lipoic acid on spermatological and biochemical parameters following equilibration and freeze-thawing of ram semen |
| title_full | Effect of lipoic acid on spermatological and biochemical parameters following equilibration and freeze-thawing of ram semen |
| title_fullStr | Effect of lipoic acid on spermatological and biochemical parameters following equilibration and freeze-thawing of ram semen |
| title_full_unstemmed | Effect of lipoic acid on spermatological and biochemical parameters following equilibration and freeze-thawing of ram semen |
| title_short | Effect of lipoic acid on spermatological and biochemical parameters following equilibration and freeze-thawing of ram semen |
| title_sort | effect of lipoic acid on spermatological and biochemical parameters following equilibration and freeze thawing of ram semen |
| topic | ram semen freezing-thawing lipoic acid |
| url | http://eurasianjvetsci.org/pdf.php3?id=572 |
| work_keys_str_mv | AT nuribaspınar effectoflipoicacidonspermatologicalandbiochemicalparametersfollowingequilibrationandfreezethawingoframsemen AT kenancoyan effectoflipoicacidonspermatologicalandbiochemicalparametersfollowingequilibrationandfreezethawingoframsemen AT mustafanumanbucak effectoflipoicacidonspermatologicalandbiochemicalparametersfollowingequilibrationandfreezethawingoframsemen AT alidoganomur effectoflipoicacidonspermatologicalandbiochemicalparametersfollowingequilibrationandfreezethawingoframsemen AT mehmetbozkurtataman effectoflipoicacidonspermatologicalandbiochemicalparametersfollowingequilibrationandfreezethawingoframsemen AT pınarpekerakalın effectoflipoicacidonspermatologicalandbiochemicalparametersfollowingequilibrationandfreezethawingoframsemen AT sukrugungor effectoflipoicacidonspermatologicalandbiochemicalparametersfollowingequilibrationandfreezethawingoframsemen AT canerozturk effectoflipoicacidonspermatologicalandbiochemicalparametersfollowingequilibrationandfreezethawingoframsemen |