Development of a transportable High-Performance Liquid Chromatograph with Ultraviolet detection and a method for the rapid analysis of 13 carbonyl compounds hydrazones
In environments with elevated air pollution, highly reactive aldehydes pose significant health concerns. This study proposes a simultaneous analysis of 13 carbonyl compounds, according to the ISO 16,000–3 reference method, adapted for a novel transportable High-Performance Liquid Chromatograph (HPLC...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-05-01
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| Series: | Journal of Chromatography Open |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772391724000884 |
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| author | Audrey Grandjean Anaïs Becker Mathilde Mascles Franck Amiet Jean-Philippe Amiet Damien Bazin Stéphane Le Calvé |
| author_facet | Audrey Grandjean Anaïs Becker Mathilde Mascles Franck Amiet Jean-Philippe Amiet Damien Bazin Stéphane Le Calvé |
| author_sort | Audrey Grandjean |
| collection | DOAJ |
| description | In environments with elevated air pollution, highly reactive aldehydes pose significant health concerns. This study proposes a simultaneous analysis of 13 carbonyl compounds, according to the ISO 16,000–3 reference method, adapted for a novel transportable High-Performance Liquid Chromatograph (HPLC) system. The transportable HPLC system consists of a reinforced analytical suitcase and a computer suitcase, each equipped with spring systems to fortify them against impact. The system features a straightforward isocratic pump, ensuring reliability and ease of operation, while sample delivery is managed by a piston pump coupled to a multiplexing valve. Derived carbonyl compounds with 2,4-dinitrophenylhydrazine were separated in isocratic mode using water and acetonitrile with a reverse-phase column in less than 20 min. Out of the 13 hydrazones, only 2-butanone-2,4-DNPH (BO-DNPH) and butanal-2,4-DNPH (BA-DNPH) were fully coeluted, and separation of the other critical pairs containing 3 and 4 carbons was achieved. The method was applied to the transportable HPLC equipped with a UV or light-emitting diode (LED) detector. These results were compared with the ones obtained on two benchtop laboratory HPLCs equipped with a diode array detector (DAD). The limit of detection (LOD) for the hydrazones of the carbonyl compounds ranged from 0.12 to 0.38 mg L–1 with the UV detector and 0.45 to 1.04 mg L–1 with the LED detector. Precision was determined from consecutive injections of the analytes to yield RSD<11.5% (UV) and RSD<14.1% (LED). Performances were close to that of the laboratory HPLCs, either in sensitivity, resolution, or repeatability with overall more accurate and robust results with the UV than with the LED detector. Despite the LED system not fully meeting the standard method requirements with linearities correlation coefficients lower than 0.999, both systems and methods were validated for the isocratic analysis of hydrazones for aldehydes and ketones quantification. An additional comparison was made with the literature regarding both the isocratic method developed and the transportable system. |
| format | Article |
| id | doaj-art-009e915b53f5479888901dba5bbbfe0b |
| institution | Kabale University |
| issn | 2772-3917 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Chromatography Open |
| spelling | doaj-art-009e915b53f5479888901dba5bbbfe0b2025-01-11T06:42:16ZengElsevierJournal of Chromatography Open2772-39172025-05-017100201Development of a transportable High-Performance Liquid Chromatograph with Ultraviolet detection and a method for the rapid analysis of 13 carbonyl compounds hydrazonesAudrey Grandjean0Anaïs Becker1Mathilde Mascles2Franck Amiet3Jean-Philippe Amiet4Damien Bazin5Stéphane Le Calvé6ICPEES – CNRS/University of Strasbourg, 25 rue Becquerel, Strasbourg, France; Chromatotec, 15 Rue d'Artiguelongue, Saint-Antoine, FranceICPEES – CNRS/University of Strasbourg, 25 rue Becquerel, Strasbourg, FranceChromatotec, 15 Rue d'Artiguelongue, Saint-Antoine, FranceChromatotec, 15 Rue d'Artiguelongue, Saint-Antoine, FranceChromatotec, 15 Rue d'Artiguelongue, Saint-Antoine, FranceChromatotec, 15 Rue d'Artiguelongue, Saint-Antoine, FranceICPEES – CNRS/University of Strasbourg, 25 rue Becquerel, Strasbourg, France; Corresponding authorIn environments with elevated air pollution, highly reactive aldehydes pose significant health concerns. This study proposes a simultaneous analysis of 13 carbonyl compounds, according to the ISO 16,000–3 reference method, adapted for a novel transportable High-Performance Liquid Chromatograph (HPLC) system. The transportable HPLC system consists of a reinforced analytical suitcase and a computer suitcase, each equipped with spring systems to fortify them against impact. The system features a straightforward isocratic pump, ensuring reliability and ease of operation, while sample delivery is managed by a piston pump coupled to a multiplexing valve. Derived carbonyl compounds with 2,4-dinitrophenylhydrazine were separated in isocratic mode using water and acetonitrile with a reverse-phase column in less than 20 min. Out of the 13 hydrazones, only 2-butanone-2,4-DNPH (BO-DNPH) and butanal-2,4-DNPH (BA-DNPH) were fully coeluted, and separation of the other critical pairs containing 3 and 4 carbons was achieved. The method was applied to the transportable HPLC equipped with a UV or light-emitting diode (LED) detector. These results were compared with the ones obtained on two benchtop laboratory HPLCs equipped with a diode array detector (DAD). The limit of detection (LOD) for the hydrazones of the carbonyl compounds ranged from 0.12 to 0.38 mg L–1 with the UV detector and 0.45 to 1.04 mg L–1 with the LED detector. Precision was determined from consecutive injections of the analytes to yield RSD<11.5% (UV) and RSD<14.1% (LED). Performances were close to that of the laboratory HPLCs, either in sensitivity, resolution, or repeatability with overall more accurate and robust results with the UV than with the LED detector. Despite the LED system not fully meeting the standard method requirements with linearities correlation coefficients lower than 0.999, both systems and methods were validated for the isocratic analysis of hydrazones for aldehydes and ketones quantification. An additional comparison was made with the literature regarding both the isocratic method developed and the transportable system.http://www.sciencedirect.com/science/article/pii/S2772391724000884Carbonyl compounds2,4-Dinitrophenylhydrazinetransportable HPLC-UVIsocratic analysisISO 16000–3 standard analysisHydrazones |
| spellingShingle | Audrey Grandjean Anaïs Becker Mathilde Mascles Franck Amiet Jean-Philippe Amiet Damien Bazin Stéphane Le Calvé Development of a transportable High-Performance Liquid Chromatograph with Ultraviolet detection and a method for the rapid analysis of 13 carbonyl compounds hydrazones Journal of Chromatography Open Carbonyl compounds 2,4-Dinitrophenylhydrazine transportable HPLC-UV Isocratic analysis ISO 16000–3 standard analysis Hydrazones |
| title | Development of a transportable High-Performance Liquid Chromatograph with Ultraviolet detection and a method for the rapid analysis of 13 carbonyl compounds hydrazones |
| title_full | Development of a transportable High-Performance Liquid Chromatograph with Ultraviolet detection and a method for the rapid analysis of 13 carbonyl compounds hydrazones |
| title_fullStr | Development of a transportable High-Performance Liquid Chromatograph with Ultraviolet detection and a method for the rapid analysis of 13 carbonyl compounds hydrazones |
| title_full_unstemmed | Development of a transportable High-Performance Liquid Chromatograph with Ultraviolet detection and a method for the rapid analysis of 13 carbonyl compounds hydrazones |
| title_short | Development of a transportable High-Performance Liquid Chromatograph with Ultraviolet detection and a method for the rapid analysis of 13 carbonyl compounds hydrazones |
| title_sort | development of a transportable high performance liquid chromatograph with ultraviolet detection and a method for the rapid analysis of 13 carbonyl compounds hydrazones |
| topic | Carbonyl compounds 2,4-Dinitrophenylhydrazine transportable HPLC-UV Isocratic analysis ISO 16000–3 standard analysis Hydrazones |
| url | http://www.sciencedirect.com/science/article/pii/S2772391724000884 |
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