Expression of dna methyltransferases (dnmts) at mrna level in ovine endometrium during estrus cycle and early pregnancy
<b>Aim: </b>To elucidate mRNA expression of DNA methyltransferase enzyme genes (DNMT1, DNMT3A, and DNMT3B) in ovine endometrium whether they are modulated during the estrus cycle and early pregnancy.<p> <b>Materials and Methods:</b> The endometrial samples including int...
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Selcuk University Press
2022-09-01
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| Series: | Eurasian Journal of Veterinary Sciences |
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| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1397 |
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| author | Mustafa Hitit Mehmet Kose |
| author_facet | Mustafa Hitit Mehmet Kose |
| author_sort | Mustafa Hitit |
| collection | DOAJ |
| description | <b>Aim: </b>To elucidate mRNA expression of DNA methyltransferase enzyme genes
(DNMT1, DNMT3A, and DNMT3B) in ovine endometrium whether they are
modulated during the estrus cycle and early pregnancy.<p>
<b>Materials and Methods:</b> The endometrial samples including intercaruncular
sites was obtained from a total of 24 ewes on days of 12 (P12, n = 4), 16 (P16,
n = 4) and 22 (P22, n = 4) of pregnancy following mating and cyclic days of
12 (C12, n = 4), 16 (C16, n = 4) and 22 (C22, n = 4) of the estrous cycle. The
relative mRNA level of DNMTs were evaluated through real-time quantitative
RT-qPCR.<p>
<b>Results: </b>Abundances of DNMTs including DNMT1, DNMT3A, and DNMT3B
were detected during the estrous cycle and early pregnancy in the ovine
endometrium by this study. DNMT1 mRNA steady-state level was greater in
P16 than in C16 whereas they did not change within the rest of the groups. The
level of DNMT3A mRNA, during early pregnancy, had the highest expression
levels on P12 compared to P22 and P16 (p<0.01). However, the level of
DNMT3A mRNA was lower in P16 than in C16 (p<0.01) and had a similar
decrease in P22 compared to C22 (p<0.01). DNMT3B mRNA level did not
differ (p>0.01) during the estrus cycle and early pregnancy.<p>
<b>Conclusion: </b>DNA methylation related DNMTs may be required to control of
gene expression in the ovine endometrium during the estrus cycle and early
pregnancy. |
| format | Article |
| id | doaj-art-00979e9a51264c36b8044c9749760fe0 |
| institution | Kabale University |
| issn | 1309-6958 2146-1953 |
| language | English |
| publishDate | 2022-09-01 |
| publisher | Selcuk University Press |
| record_format | Article |
| series | Eurasian Journal of Veterinary Sciences |
| spelling | doaj-art-00979e9a51264c36b8044c9749760fe02025-01-02T22:36:19ZengSelcuk University PressEurasian Journal of Veterinary Sciences1309-69582146-19532022-09-013831431491397Expression of dna methyltransferases (dnmts) at mrna level in ovine endometrium during estrus cycle and early pregnancyMustafa Hitit0Mehmet Kose1Kastamonu University, Veterinary Faculty, Department of Genetic, Kastamonu, TurkeyDicle University, Veterinary Faculty, Department of Obstetrics and Gynaecology, Dicle, Turkey<b>Aim: </b>To elucidate mRNA expression of DNA methyltransferase enzyme genes (DNMT1, DNMT3A, and DNMT3B) in ovine endometrium whether they are modulated during the estrus cycle and early pregnancy.<p> <b>Materials and Methods:</b> The endometrial samples including intercaruncular sites was obtained from a total of 24 ewes on days of 12 (P12, n = 4), 16 (P16, n = 4) and 22 (P22, n = 4) of pregnancy following mating and cyclic days of 12 (C12, n = 4), 16 (C16, n = 4) and 22 (C22, n = 4) of the estrous cycle. The relative mRNA level of DNMTs were evaluated through real-time quantitative RT-qPCR.<p> <b>Results: </b>Abundances of DNMTs including DNMT1, DNMT3A, and DNMT3B were detected during the estrous cycle and early pregnancy in the ovine endometrium by this study. DNMT1 mRNA steady-state level was greater in P16 than in C16 whereas they did not change within the rest of the groups. The level of DNMT3A mRNA, during early pregnancy, had the highest expression levels on P12 compared to P22 and P16 (p<0.01). However, the level of DNMT3A mRNA was lower in P16 than in C16 (p<0.01) and had a similar decrease in P22 compared to C22 (p<0.01). DNMT3B mRNA level did not differ (p>0.01) during the estrus cycle and early pregnancy.<p> <b>Conclusion: </b>DNA methylation related DNMTs may be required to control of gene expression in the ovine endometrium during the estrus cycle and early pregnancy.http://eurasianjvetsci.org/pdf.php3?id=1397dnmt1dnmt3adnmt3bovine endometriumgene expression |
| spellingShingle | Mustafa Hitit Mehmet Kose Expression of dna methyltransferases (dnmts) at mrna level in ovine endometrium during estrus cycle and early pregnancy Eurasian Journal of Veterinary Sciences dnmt1 dnmt3a dnmt3b ovine endometrium gene expression |
| title | Expression of dna methyltransferases (dnmts) at mrna level in ovine endometrium during estrus cycle and early pregnancy |
| title_full | Expression of dna methyltransferases (dnmts) at mrna level in ovine endometrium during estrus cycle and early pregnancy |
| title_fullStr | Expression of dna methyltransferases (dnmts) at mrna level in ovine endometrium during estrus cycle and early pregnancy |
| title_full_unstemmed | Expression of dna methyltransferases (dnmts) at mrna level in ovine endometrium during estrus cycle and early pregnancy |
| title_short | Expression of dna methyltransferases (dnmts) at mrna level in ovine endometrium during estrus cycle and early pregnancy |
| title_sort | expression of dna methyltransferases dnmts at mrna level in ovine endometrium during estrus cycle and early pregnancy |
| topic | dnmt1 dnmt3a dnmt3b ovine endometrium gene expression |
| url | http://eurasianjvetsci.org/pdf.php3?id=1397 |
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