Construction of a high-sensitivity Cherenkov luminescence endoscopy system for the detection of gastrointestinal cancers
Abstract Background The diagnostic yield of conventional gastrointestinal (GI) endoscopy for early cancers is low because it is mainly based on morphological changes of tumors. Molecular functional changes in tumors precede morphological changes. Cherenkov luminescence endoscopy (CLE) system can per...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2025-04-01
|
| Series: | EJNMMI Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13550-025-01223-9 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849734713909968896 |
|---|---|
| author | Ze Yang Tian-Tian Pang Zhuo-Jun Wu Tian-Yu Yan Jing-Min Yu Xin-Yu Wang Dan Liu Xiao-Jian Lu Xiao-Yu Kang Gui-Yu Li Cheng Bai Xiao-Juan Xi Zu-Hong Tian Yu Qi Ming-Ru Zhang Fei Kang Jing Wang Xue-Li Chen Kai-Chun Wu |
| author_facet | Ze Yang Tian-Tian Pang Zhuo-Jun Wu Tian-Yu Yan Jing-Min Yu Xin-Yu Wang Dan Liu Xiao-Jian Lu Xiao-Yu Kang Gui-Yu Li Cheng Bai Xiao-Juan Xi Zu-Hong Tian Yu Qi Ming-Ru Zhang Fei Kang Jing Wang Xue-Li Chen Kai-Chun Wu |
| author_sort | Ze Yang |
| collection | DOAJ |
| description | Abstract Background The diagnostic yield of conventional gastrointestinal (GI) endoscopy for early cancers is low because it is mainly based on morphological changes of tumors. Molecular functional changes in tumors precede morphological changes. Cherenkov luminescence endoscopy (CLE) system can perform molecular imaging of GI cancers, achieving early diagnosis of cancers. However, previous CLE systems had only been able to detect Cherenkov luminescence (CL) from about one μCi nuclide at a minimum (in vivo), but the nuclide probe absorbed by the tumor of a patient was often much less than one μCi at a routinely administered dose. This study aims to construct a clinically usable high-sensitivity CLE for molecular imaging of GI cancers. Results The minimum resolvable radioactivity of the CLE reached 0.020 μCi within 300 s (in vivo), with a sensitivity at the nanocurie for the first time. The detection sensitivity of the CLE increased by up to nearly twenty-two times over the previous system. In tumor-bearing nude mice, CLE could effectively identify all tumors with 100% concordance with both histopathology and PET/CT, and the CL signals of tumors were much stronger than those of the surrounding normal tissues (P < 0.05). The quality of CLE imaging at 60 s was comparable to that at 300 s (signal-to-background ratio, 2.70 ± 0.48 versus 2.98 ± 0.69, P = 0.56). Conclusions We constructed a high-sensitivity CLE that could detect radionuclides at the nanocurie radioactivity. The CLE could detect cancers accurately through rapid molecular imaging and had the potential for early diagnosis of GI cancers in clinical practice. |
| format | Article |
| id | doaj-art-008e7ceefebe4fda9967b78f3c764cab |
| institution | DOAJ |
| issn | 2191-219X |
| language | English |
| publishDate | 2025-04-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | EJNMMI Research |
| spelling | doaj-art-008e7ceefebe4fda9967b78f3c764cab2025-08-20T03:07:44ZengSpringerOpenEJNMMI Research2191-219X2025-04-0115111510.1186/s13550-025-01223-9Construction of a high-sensitivity Cherenkov luminescence endoscopy system for the detection of gastrointestinal cancersZe Yang0Tian-Tian Pang1Zhuo-Jun Wu2Tian-Yu Yan3Jing-Min Yu4Xin-Yu Wang5Dan Liu6Xiao-Jian Lu7Xiao-Yu Kang8Gui-Yu Li9Cheng Bai10Xiao-Juan Xi11Zu-Hong Tian12Yu Qi13Ming-Ru Zhang14Fei Kang15Jing Wang16Xue-Li Chen17Kai-Chun Wu18State Key Laboratory of Holistic Integrative Management of Gastrointestinal Cancers and National Clinical Research Center for Digestive Diseases, Xijing Hospital of Digestive Diseases, Fourth Military Medical UniversityNorthwestern Polytechnical UniversityState Key Laboratory of Holistic Integrative Management of Gastrointestinal Cancers and National Clinical Research Center for Digestive Diseases, Xijing Hospital of Digestive Diseases, Fourth Military Medical UniversityEngineering Research Center of Molecular and Neuro Imaging of Ministry of Education & School of Life Science and Technology, Xidian UniversityState Key Laboratory of Holistic Integrative Management of Gastrointestinal Cancers and National Clinical Research Center for Digestive Diseases, Xijing Hospital of Digestive Diseases, Fourth Military Medical UniversityEngineering Research Center of Molecular and Neuro Imaging of Ministry of Education & School of Life Science and Technology, Xidian UniversityState Key Laboratory of Holistic Integrative Management of Gastrointestinal Cancers and National Clinical Research Center for Digestive Diseases, Xijing Hospital of Digestive Diseases, Fourth Military Medical UniversityNanjing Chunhui Science and Technology Industrial Co. LtdState Key Laboratory of Holistic Integrative Management of Gastrointestinal Cancers and National Clinical Research Center for Digestive Diseases, Xijing Hospital of Digestive Diseases, Fourth Military Medical UniversityDepartment of Nuclear Medicine, Xijing Hospital, Fourth Military Medical UniversityDepartment of Gastroenterology, 967th Hospital of the PLA Joint Logistic Support ForceState Key Laboratory of Holistic Integrative Management of Gastrointestinal Cancers and National Clinical Research Center for Digestive Diseases, Xijing Hospital of Digestive Diseases, Fourth Military Medical UniversityState Key Laboratory of Holistic Integrative Management of Gastrointestinal Cancers and National Clinical Research Center for Digestive Diseases, Xijing Hospital of Digestive Diseases, Fourth Military Medical UniversityState Key Laboratory of Holistic Integrative Management of Gastrointestinal Cancers and National Clinical Research Center for Digestive Diseases, Xijing Hospital of Digestive Diseases, Fourth Military Medical UniversityDepartment of Nuclear Medicine, Xijing Hospital, Fourth Military Medical UniversityDepartment of Nuclear Medicine, Xijing Hospital, Fourth Military Medical UniversityDepartment of Nuclear Medicine, Xijing Hospital, Fourth Military Medical UniversityEngineering Research Center of Molecular and Neuro Imaging of Ministry of Education & School of Life Science and Technology, Xidian UniversityState Key Laboratory of Holistic Integrative Management of Gastrointestinal Cancers and National Clinical Research Center for Digestive Diseases, Xijing Hospital of Digestive Diseases, Fourth Military Medical UniversityAbstract Background The diagnostic yield of conventional gastrointestinal (GI) endoscopy for early cancers is low because it is mainly based on morphological changes of tumors. Molecular functional changes in tumors precede morphological changes. Cherenkov luminescence endoscopy (CLE) system can perform molecular imaging of GI cancers, achieving early diagnosis of cancers. However, previous CLE systems had only been able to detect Cherenkov luminescence (CL) from about one μCi nuclide at a minimum (in vivo), but the nuclide probe absorbed by the tumor of a patient was often much less than one μCi at a routinely administered dose. This study aims to construct a clinically usable high-sensitivity CLE for molecular imaging of GI cancers. Results The minimum resolvable radioactivity of the CLE reached 0.020 μCi within 300 s (in vivo), with a sensitivity at the nanocurie for the first time. The detection sensitivity of the CLE increased by up to nearly twenty-two times over the previous system. In tumor-bearing nude mice, CLE could effectively identify all tumors with 100% concordance with both histopathology and PET/CT, and the CL signals of tumors were much stronger than those of the surrounding normal tissues (P < 0.05). The quality of CLE imaging at 60 s was comparable to that at 300 s (signal-to-background ratio, 2.70 ± 0.48 versus 2.98 ± 0.69, P = 0.56). Conclusions We constructed a high-sensitivity CLE that could detect radionuclides at the nanocurie radioactivity. The CLE could detect cancers accurately through rapid molecular imaging and had the potential for early diagnosis of GI cancers in clinical practice.https://doi.org/10.1186/s13550-025-01223-9Cherenkov luminescence endoscopyCherenkov luminescence imagingGastrointestinal endoscopyMolecular imagingDiagnosis of gastrointestinal cancers |
| spellingShingle | Ze Yang Tian-Tian Pang Zhuo-Jun Wu Tian-Yu Yan Jing-Min Yu Xin-Yu Wang Dan Liu Xiao-Jian Lu Xiao-Yu Kang Gui-Yu Li Cheng Bai Xiao-Juan Xi Zu-Hong Tian Yu Qi Ming-Ru Zhang Fei Kang Jing Wang Xue-Li Chen Kai-Chun Wu Construction of a high-sensitivity Cherenkov luminescence endoscopy system for the detection of gastrointestinal cancers EJNMMI Research Cherenkov luminescence endoscopy Cherenkov luminescence imaging Gastrointestinal endoscopy Molecular imaging Diagnosis of gastrointestinal cancers |
| title | Construction of a high-sensitivity Cherenkov luminescence endoscopy system for the detection of gastrointestinal cancers |
| title_full | Construction of a high-sensitivity Cherenkov luminescence endoscopy system for the detection of gastrointestinal cancers |
| title_fullStr | Construction of a high-sensitivity Cherenkov luminescence endoscopy system for the detection of gastrointestinal cancers |
| title_full_unstemmed | Construction of a high-sensitivity Cherenkov luminescence endoscopy system for the detection of gastrointestinal cancers |
| title_short | Construction of a high-sensitivity Cherenkov luminescence endoscopy system for the detection of gastrointestinal cancers |
| title_sort | construction of a high sensitivity cherenkov luminescence endoscopy system for the detection of gastrointestinal cancers |
| topic | Cherenkov luminescence endoscopy Cherenkov luminescence imaging Gastrointestinal endoscopy Molecular imaging Diagnosis of gastrointestinal cancers |
| url | https://doi.org/10.1186/s13550-025-01223-9 |
| work_keys_str_mv | AT zeyang constructionofahighsensitivitycherenkovluminescenceendoscopysystemforthedetectionofgastrointestinalcancers AT tiantianpang constructionofahighsensitivitycherenkovluminescenceendoscopysystemforthedetectionofgastrointestinalcancers AT zhuojunwu constructionofahighsensitivitycherenkovluminescenceendoscopysystemforthedetectionofgastrointestinalcancers AT tianyuyan constructionofahighsensitivitycherenkovluminescenceendoscopysystemforthedetectionofgastrointestinalcancers AT jingminyu constructionofahighsensitivitycherenkovluminescenceendoscopysystemforthedetectionofgastrointestinalcancers AT xinyuwang constructionofahighsensitivitycherenkovluminescenceendoscopysystemforthedetectionofgastrointestinalcancers AT danliu constructionofahighsensitivitycherenkovluminescenceendoscopysystemforthedetectionofgastrointestinalcancers AT xiaojianlu constructionofahighsensitivitycherenkovluminescenceendoscopysystemforthedetectionofgastrointestinalcancers AT xiaoyukang constructionofahighsensitivitycherenkovluminescenceendoscopysystemforthedetectionofgastrointestinalcancers AT guiyuli constructionofahighsensitivitycherenkovluminescenceendoscopysystemforthedetectionofgastrointestinalcancers AT chengbai constructionofahighsensitivitycherenkovluminescenceendoscopysystemforthedetectionofgastrointestinalcancers AT xiaojuanxi constructionofahighsensitivitycherenkovluminescenceendoscopysystemforthedetectionofgastrointestinalcancers AT zuhongtian constructionofahighsensitivitycherenkovluminescenceendoscopysystemforthedetectionofgastrointestinalcancers AT yuqi constructionofahighsensitivitycherenkovluminescenceendoscopysystemforthedetectionofgastrointestinalcancers AT mingruzhang constructionofahighsensitivitycherenkovluminescenceendoscopysystemforthedetectionofgastrointestinalcancers AT feikang constructionofahighsensitivitycherenkovluminescenceendoscopysystemforthedetectionofgastrointestinalcancers AT jingwang constructionofahighsensitivitycherenkovluminescenceendoscopysystemforthedetectionofgastrointestinalcancers AT xuelichen constructionofahighsensitivitycherenkovluminescenceendoscopysystemforthedetectionofgastrointestinalcancers AT kaichunwu constructionofahighsensitivitycherenkovluminescenceendoscopysystemforthedetectionofgastrointestinalcancers |