Triplicate PCR reactions for 16S rRNA gene amplicon sequencing are unnecessary by Clarisse Marotz, Anukriti Sharma, Greg Humphrey, Neil Gottel, Christopher Daum, Jack A Gilbert, Emiley Eloe-Fadrosh, Rob Knight

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Stool multi-omics for the study of host–microbe interactions in inflammatory bowel disease by Consuelo Sauceda, Charlie Bayne, Khadijeh Sudqi, Antonio Gonzalez, Parambir S. Dulai, Rob Knight, David J. Gonzalez, Carlos G. Gonzalez

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Advances in artificial intelligence and precision nutrition approaches to improve maternal and child health in low resource settings by Saurabh Mehta, Samantha L. Huey, Shah Mohammad Fahim, Srishti Sinha, Kripa Rajagopalan, Tahmeed Ahmed, Rob Knight, Julia L. Finkelstein

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Gut microbiota and derived metabolites mediate obstructive sleep apnea induced atherosclerosis by Jin Xue, Celeste Allaband, Simone Zuffa, Orit Poulsen, Jason Meadows, Dan Zhou, Pieter C. Dorrestein, Rob Knight, Gabriel G. Haddad

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TEMPTED: time-informed dimensionality reduction for longitudinal microbiome studies by Pixu Shi, Cameron Martino, Rungang Han, Stefan Janssen, Gregory Buck, Myrna Serrano, Kouros Owzar, Rob Knight, Liat Shenhav, Anru R. Zhang

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Environmental reservoirs of pathogenic mycobacteria across the Ethiopian biogeographical landscape. by Hayley C King, Tanya Khera-Butler, Phillip James, Brian B Oakley, Girume Erenso, Abraham Aseffa, Rob Knight, Elizabeth M Wellington, Orin Courtenay

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Gut Microbiome as a Risk Factor for Future CKD by Tapio Hellman, Li-Fang Yeo, Joonatan Palmu, Aki Havulinna, Pekka Jousilahti, Ville Laitinen, Katariina Pärnänen, Veikko Salomaa, Leo Lahti, Rob Knight, Teemu Niiranen

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Correction for Tang-Wing et al., “Impact of diet change on the gut microbiome of common marmosets (Callithrix jacchus)” by Cassandra Tang-Wing, Ipsita Mohanty, MacKenzie Bryant, Katherine Makowski, Daira Melendez, Pieter C. Dorrestein, Rob Knight, Andrés Mauricio Caraballo-Rodríguez, Celeste Allaband, Keith Jenné

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Correction for Brennan et al., “Maximizing the potential of high-throughput next-generation sequencing through precise normalization based on read count distribution” by Caitriona Brennan, Rodolfo A. Salido, Pedro Belda-Ferre, MacKenzie Bryant, Charles Cowart, Maria D. Tiu, Antonio Gonzalez, Daniel McDonald, Caitlin Tribelhorn, Amir Zarrinpar, Rob Knight

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Interacting symbionts and immunity in the amphibian skin mucosome predict disease risk and probiotic effectiveness. by Douglas C Woodhams, Hannelore Brandt, Simone Baumgartner, Jos Kielgast, Eliane Küpfer, Ursina Tobler, Leyla R Davis, Benedikt R Schmidt, Christian Bel, Sandro Hodel, Rob Knight, Valerie McKenzie

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Variation and prognostic potential of the gut antibiotic resistome in the FINRISK 2002 cohort by Katariina Pärnänen, Matti O. Ruuskanen, Guilhem Sommeria-Klein, Ville Laitinen, Pyry Kantanen, Guillaume Méric, Camila Gazolla Volpiano, Michael Inouye, Rob Knight, Veikko Salomaa, Aki S. Havulinna, Teemu Niiranen, Leo Lahti

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Chronological age estimation from human microbiomes with transformer-based Robust Principal Component Analysis by Tyler Myers, Se Jin Song, Yang Chen, Britta De Pessemier, Lora Khatib, Daniel McDonald, Shi Huang, Richard Gallo, Chris Callewaert, Aki S. Havulinna, Leo Lahti, Guus Roeselers, Manolo Laiola, Sudarshan A. Shetty, Scott T. Kelley, Rob Knight, Andrew Bartko

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Blood microbial DNA signature differentiates hepatocellular carcinoma from metastatic lesions by Rohit Loomba, Rob Knight, Kit Curtius, Amir Zarrinpar, Sergio Duarte, Caitlin Guccione, Ana Carolina Dantas Machado, Fady Youssef, Isabella Angeli-Pahim, Curtis Warren, Sawyer Farmer, Gregory Humphrey, Roland Alexander Richter, Daniel McDonald, Yuhan Weng, Adam Burgoyne, Ali Zarrinpar

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Correction for Taylor et al., “Depression in Individuals Coinfected with HIV and HCV Is Associated with Systematic Differences in the Gut Microbiome and Metabolome” by Bryn C. Taylor, Kelly C. Weldon, Ronald J. Ellis, Donald Franklin, Tobin Groth, Emily C. Gentry, Anupriya Tripathi, Daniel McDonald, Gregory Humphrey, MacKenzie Bryant, Julia Toronczak, Tara Schwartz, Michelli F. Oliveira, Robert Heaton, Igor Grant, Sara Gianella, Scott Letendre, Austin Swafford, Pieter C. Dorrestein, Rob Knight

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Consuming a modified Mediterranean ketogenic diet reverses the peripheral lipid signature of Alzheimer’s disease in humans by Bryan J. Neth, Kevin Huynh, Corey Giles, Tingting Wang, Natalie A. Mellett, Thy Duong, Colette Blach, Leyla Schimmel, Thomas C. Register, Kaj Blennow, Henrik Zetterberg, Richa Batra, Annalise Schweickart, Amanda Hazel Dilmore, Cameron Martino, Matthias Arnold, Jan Krumsiek, Xianlin Han, Pieter C. Dorrestein, Rob Knight, Peter J. Meikle, Suzanne Craft, Rima Kaddurah-Daouk

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The microbiota vault initiative: safeguarding Earth’s microbial heritage for future generations by Maria G. Dominguez-Bello, Dominik Steiger, Manuel Fankhauser, Adrian Egli, Pascale Vonaesch, Nicholas A. Bokulich, Anton Lavrinienko, Christian Hoffmann, Petra Zimmermann, Abdifatah Muhummed, Somphou Sayasone, Amma Larbi, Segla Wilfrid Padonou, Jakob Zinsstag, Rea Tschopp, Chalat Santivarangkna, Diana Albertos Torres, Yuen Yi Li, Vanni Benvenga, Youzheng Teo, Marcel Houngbédji, Alex Kwarteng, Rob Knight, Jack A. Gilbert, Martin J. Blaser

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Correction for Zhu et al., “Phylogeny-Aware Analysis of Metagenome Community Ecology Based on Matched Reference Genomes while Bypassing Taxonomy” by Qiyun Zhu, Shi Huang, Antonio Gonzalez, Imran McGrath, Daniel McDonald, Niina Haiminen, George Armstrong, Yoshiki Vázquez-Baeza, Julian Yu, Justin Kuczynski, Gregory D. Sepich-Poore, Austin D. Swafford, Promi Das, Justin P. Shaffer, Franck Lejzerowicz, Pedro Belda-Ferre, Aki S. Havulinna, Guillaume Méric, Teemu Niiranen, Leo Lahti, Veikko Salomaa, Ho-Cheol Kim, Mohit Jain, Michael Inouye, Jack A. Gilbert, Rob Knight

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Experiences and lessons learned from two virtual, hands-on microbiome bioinformatics workshops. by Matthew R Dillon, Evan Bolyen, Anja Adamov, Aeriel Belk, Emily Borsom, Zachary Burcham, Justine W Debelius, Heather Deel, Alex Emmons, Mehrbod Estaki, Chloe Herman, Christopher R Keefe, Jamie T Morton, Renato R M Oliveira, Andrew Sanchez, Anthony Simard, Yoshiki Vázquez-Baeza, Michal Ziemski, Hazuki E Miwa, Terry A Kerere, Carline Coote, Richard Bonneau, Rob Knight, Guilherme Oliveira, Piraveen Gopalasingam, Benjamin D Kaehler, Emily K Cope, Jessica L Metcalf, Michael S Robeson Ii, Nicholas A Bokulich, J Gregory Caporaso

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A three-country analysis of the gut microbiome indicates taxon associations with diet vary by taxon resolution and population by Lora Khatib, Se Jin Song, Amanda H. Dilmore, Jon G. Sanders, Caitriona Brennan, Alejandra Rios Hernandez, Tyler Myers, Renee Oles, Sawyer Farmer, Charles Cowart, Amanda Birmingham, Edgar A. Diaz, Oliver Nizet, Kat Gilbert, Nicole Litwin, Promi Das, Brent Nowinski, Mackenzie Bryant, Caitlin Tribelhorn, Karenina Sanders-Bodai, Soline Chaumont, Jan Knol, Guus Roeselers, Manolo Laiola, Sudarshan A. Shetty, Patrick Veiga, Julien Tap, Muriel Derrien, Hana Koutnikova, Aurélie Cotillard, Christophe Lay, Armando R. Tovar, Nimbe Torres, Liliana Arteaga, Antonio González, Daniel McDonald, Andrew Bartko, Rob Knight

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Phylogeographic and genetic network assessment of COVID-19 mitigation protocols on SARS-CoV-2 transmission in university campus residencesResearch in context by Joel O. Wertheim, Tetyana I. Vasylyeva, Robert J. Wood, Kalen Cantrell, Soraya Piña Contreras, Aryeh Feldheim, Ravi Goyal, Jennifer L. Havens, Rob Knight, Louise C. Laurent, Niema Moshiri, Robert Neuhard, Shashank Sathe, Alysson Satterlund, Angela Scioscia, Angela Y. Song, Robert T. Schooley, Cheryl M. Anderson, Natasha K. Martin, Stefan Aigner, Kristian G. Andersen, Nathan A. Baer, Maryann Betty, Amanda Birmingham, Anelizze Castro-Martinez, Willi Cheung, Peter De Hoff, Kathleen M. Fisch, Alison J. King, Karthik Gangavarapu, Abbas Hakim, Benjamin Henson, Kristen Jepsen, Christina H. Mac, Toan T. Ngo, Kelly N. Nguyen, Tyler R. Ostrander, Sarah Perkins, Ashley Plascencia, Andrea Rivera, Ariana Rivera, Rodolfo A. Salido, Kieran C. Saucedo, Madison Schwab, Allison L. Steedman, Anthony Veder, Alana Weiss, Gene W. Yeo, Mark Zeller

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